scholarly journals Under an integrative taxonomic approach: the description of a new species of the genus Loxosceles (Araneae, Sicariidae) from Mexico City

ZooKeys ◽  
2019 ◽  
Vol 892 ◽  
pp. 93-133 ◽  
Author(s):  
Alejandro Valdez-Mondragón ◽  
Claudia I. Navarro-Rodríguez ◽  
Karen P. Solís-Catalán ◽  
Mayra R. Cortez-Roldán ◽  
Alma R. Juárez-Sánchez
Keyword(s):  
New Species ◽  
Mexico City ◽  
Molecular Species ◽  
Species Delimitation ◽  
Morphological Characters ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Taxonomic Approach ◽  
Unique Species ◽  
A New Species

A new species of the spider genus Loxosceles Heineken & Lowe, 1832, Loxosceles tenochtitlan Valdez-Mondragón & Navarro-Rodríguez, sp. nov., is described based on adult male and female specimens from the states of Mexico City, Estado de Mexico and Tlaxcala. Integrative taxonomy including traditional morphology, geometric and lineal morphology, and molecules (DNA barcodes of cytochrome c oxidase subunit 1 (CO1) and internal transcribed spacer 2 (ITS2)), were used as evidence to delimit the new species. Four methods were used for molecular analyses and species delimitation: 1) corrected p-distances under neighbor joining (NJ), 2) automatic barcode gap discovery (ABGD), 3) general mixed yule coalescent model (GMYC), and 4) poisson tree processes (bPTP). All molecular methods, traditional, geometric and lineal morphology were consistent in delimiting and recognizing the new species. Loxosceles tenochtitlansp. nov. is closely related to L. misteca based on molecular data. Although both species are morphologically similar, the average p-distance from CO1 data was 13.8% and 4.2% for ITS2 data. The molecular species delimitation methods recovered well-supported monophyletic clusters for samples of L. tenochtitlansp. nov. from Mexico City + Tlaxcala and for samples of L. misteca from Guerrero. Loxosceles tenochtitlansp. nov. is considered a unique species for three reasons: (1) it can be distinguished by morphological characters (genitalic and somatic); (2) the four different molecular species delimitation methods were congruent to separate both species; and (3) there is variation in leg I length of males between both species, with the males of L. misteca having longer legs than males of L. tenochtitlansp. nov., also morphometrically, the shape of tibiae of the palp between males of both species is different.

scholarly journals Description of a new species of Loxosceles Heineken & Lowe (Araneae, Sicariidae) recluse spiders from Hidalgo, Mexico, under integrative taxonomy: morphological and DNA barcoding data (CO1 + ITS2)

2020 ◽  
Author(s):  
Claudia Isabel Navarro-Rodríguez ◽  
Alejandro Valdez-Mondragón
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Species Delimitation ◽  
Sister Group ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Taxonomic Approach ◽  
Morphological Differences ◽  
Unique Species ◽  
A New Species

Based on an integrative taxonomic approach, a new species of the genus Loxosceles Heineken & Lowe, 1832, is described from the state of Hidalgo, Mexico. Loxosceles tolantongo sp. nov. is described based on DNA barcoding using cytochrome c oxidase subunit 1 (CO1) and internal transcribed spacer 2 (ITS2), and morphology. For species delimitation, four molecular methods were implemented: 1) corrected p-distances under neighbor joining (NJ); 2) automatic barcode gap discovery (ABGD); 3) general mixed yule coalescent model (GMYC) and 4) Bayesian Poisson tree processes (bPTP). The new species morphologically resembles L. jaca, another species from Hidalgo, but there are morphological differences mainly in the tibiae of the male palp, the seminal receptacles of the females and also the high genetic p-distances. CO1 was more informative than ITS2 for the genetic separation; however, both concatenated genes (CO1 + ITS2) present robust evidence for species delimitation. Loxosceles tolantongo sp. nov. is considered a unique species for four reasons: 1) it can be diagnosed and distinguished by morphological characters (of the male palps mainly, but also of the seminal receptacles of the females); 2) the genetic p-distances with CO1 were high (>10%); 3) the molecular species delimitation methods were congruent under CO1 and CO1 + ITS2; and 4) under CO1 and CO1 + ITS2, the new species is a putative sister group of L. jaca + L. tenango.

scholarly journals Integrative taxonomy of the genus Pseudostegana (Diptera, Drosophilidae) from China, with descriptions of eleven new species

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5160 ◽  
Author(s):  
Jinming Lu ◽  
Yuan Zhang ◽  
Hongwei Chen
Keyword(s):  
New Species ◽  
Species Delimitation ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Southwestern China ◽  
28S Rrna ◽  
Nuclear Locus ◽  
Barcode Gap

The genus Pseudostegana (Okada, 1978) currently contains thirty-nine described species. A number of Pseudostegana were collected from the fieldwork in southwestern China from 2010 to 2017. Eleven new species were discovered and are described from southwestern China: Pseudostegana alpina Zhang & Chen, sp. nov.; Pseudostegana amnicola Zhang & Chen, sp. nov.; Pseudostegana amoena Zhang & Chen, sp. nov.; Pseudostegana mailangang Zhang & Chen, sp. nov.; Pseudostegana meiduo Zhang & Chen, sp. nov.; Pseudostegana meiji Zhang & Chen, sp. nov.; Pseudostegana mystica Zhang & Chen, sp. nov.; Pseudostegana stictiptrata Zhang & Chen, sp. nov.; Pseudostegana stigmatptera Zhang & Chen, sp. nov.; Pseudostegana ximalaya Zhang & Chen, sp. nov. and Pseudostegana zhuoma Zhang & Chen, sp. nov. A key to all Chinese Pseudostegana species based on morphological characters is provided. Two mitochondrial loci (COI and ND2) and one nuclear locus (28S rRNA) were sequenced for the Pseudostegana specimens, and Bayesian and RAxML concatenated analyses were run. Molecular species delimitation is performed using the distance-based automatic barcode gap discovery (ABGD) method. Molecular data support the morphological characteristics observed among these Chinese species and confirm the new species as being distinctly different.

scholarly journals Integrative taxonomy of the rock-dwelling gecko Cnemaspis siamensis complex (Squamata, Gekkonidae) reveals a new species from Nakhon Si Thammarat Province, southern Thailand

ZooKeys ◽  
2020 ◽  
Vol 932 ◽  
pp. 129-159
Author(s):  
Natee Ampai ◽  
Perry L. Wood Jr ◽  
Bryan L. Stuart ◽  
Anchalee Aowphol
Keyword(s):  
New Species ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Morphological Data ◽  
Mountain Range ◽  
Adult Males ◽  
Nadh Dehydrogenase Subunit 2 ◽  
Southern Thailand ◽  
Taxonomic Approach ◽  
A New Species

The rock-dwelling gecko genus Cnemaspis is one of the most species-diverse genera of gekkonid in Thailand. Earlier studies relied on morphological data to identify species, but cryptic morphology often obscured species diversity in Cnemaspis. In this study, an integrative taxonomic approach based on morphological characters and sequences of the mitochondrial NADH dehydrogenase subunit 2 (ND2) gene were used to clarify current taxonomy of the Cnemaspis siamensis complex and delimit a new species from Lan Saka District, Nakhon Si Thammarat Province, southern Thailand. Cnemaspis lineatubercularissp. nov. is distinguished from other congeneric species by the combination of morphological characters: (1) maximum snout-vent length (SVL) of 40.6 mm (mean 38.8 ± SD 1.4, N = 12) in adult males and maximum SVL of 41.8 mm (mean 39.5 ± SD 1.9, N = 7) in adult females; (2) 8–9 supralabial and infralabial scales; (3) gular, pectoral, abdominal, and subcaudal scales keeled; (4) rostral, interorbitals, supercilium, palmar scales, and ventral scales of brachia smooth; (5) 5–6 small, subconical spine-like tubercles present on flanks; (6) 19–21 paravertebral tubercles linearly arranged; (7) 27–29 subdigital lamellae under the fourth toe; (8) 4–7 pore-bearing precloacal scales, pores rounded arranged in chevron shape and separated only in males; (9) one postcloacal tubercles each side in males; (10) ventrolateral caudal tubercles present anteriorly; (11) caudal tubercles restricted to a single paravertebral row on each side; (12) single median row of subcaudal scales keeled and lacking enlarged median row; and (13) gular region, abdomen, limbs and subcaudal region yellowish only in males. Genetically, the uncorrected pairwise divergences between the new species and their congeners in the C. siamensis group were between 15.53–28.09%. The new species is currently known only from granitic rocky streams at Wang Mai Pak Waterfall in the Nakhon Si Thammarat mountain range. Its discovery suggests that additional unrecognized species of Cnemaspis may still occur in unexplored areas of southern Thailand.

scholarly journals Integrative taxonomy by molecular species delimitation: multi-locus data corroborate a new species of Balkan Drusinae micro-endemics

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Simon Vitecek ◽  
Mladen Kučinić ◽  
Ana Previšić ◽  
Ivana Živić ◽  
Katarina Stojanović ◽  
...  
Keyword(s):  
New Species ◽  
Molecular Species ◽  
Species Delimitation ◽  
Integrative Taxonomy ◽  
A New Species

A new species of Scrophularia (Scrophulariaceae) from Hubei, China

Phytotaxa ◽  
2018 ◽  
Vol 350 (1) ◽  
pp. 1
Author(s):  
RUI-HONG WANG ◽  
MAO-QIN XIA ◽  
JIN-BO TAN ◽  
CHUAN CHEN ◽  
XIN-JIE JIN ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Species Delimitation ◽  
Morphological Characters ◽  
Central China ◽  
Molecular Phylogenetic Analysis ◽  
Leaf Margin ◽  
Close Relationship ◽  
Molecular Phylogenetic ◽  
A New Species

A new species, Scrophularia jinii (Scrophulariaceae), from Central China is described and illustrated. This new species was formerly misidentified as S. fargesii, from which it differs in many morphological characters. Moreover, it is distinct with all known Scrophularia species in its unique deeply double serrate leaf margin with 3–7 big teeth on each side. Molecular phylogenetic analysis further supports its species delimitation and suggests a close relationship with several Japanese and North American species.

scholarly journals Phylogeography and Molecular Species Delimitation of Pratylenchus capsici n. sp., a New Root-Lesion Nematode in Israel on Pepper (Capsicum annuum)

2019 ◽  
Vol 109 (5) ◽  
pp. 847-858 ◽  
Author(s):  
Xue Qing ◽  
Wim Bert ◽  
Abraham Gamliel ◽  
Patricia Bucki ◽  
Svetlana Duvrinin ◽  
...  
Keyword(s):  
New Species ◽  
Capsicum Annuum ◽  
Molecular Species ◽  
Species Delimitation ◽  
Chenopodium Album ◽  
Haplotype Diversity ◽  
Small Scale ◽  
Growing Seasons ◽  
Contemporary Gene Flow ◽  
A New Species

Root-lesion nematodes of the genus Pratylenchus parasitize the roots of numerous plants and can cause severe damage and yield loss. Here, we report on a new species, Pratylenchus capsici n. sp., from the Arava rift, Israel, which was characterized by integrative methods, including detailed morphology, molecular phylogeny, population genetics, and phylogeography. This species is widely spread across the Arava rift, causing significant infection in pepper (Capsicum annuum) roots and inhibiting plant growth. Both morphological and molecular species delimitation support the recovered species as a new species. We found high cytochrome oxidase subunit I haplotype diversity, and phylogeography analysis suggests that contemporary gene flow is prevented among different agricultural farms, while population dispersal from weeds (Chenopodium album and Sonchus oleraceus) to pepper occurs on a relatively small scale. Our results suggest that weeds are an important reservoir for the dispersal of P. capsici n. sp., either as the original nematode source or at least in maintaining the population between growing seasons.

Description of two new species of Rhamphus related to R. oxyacanthae (Curculionidae, Curculioninae, Rhamphini) from Italy based on a morphological study supported by molecular data

Zootaxa ◽  
2021 ◽  
Vol 4995 (1) ◽  
pp. 111-128
Author(s):  
LUCIANO DIOTTI ◽  
ROBERTO CALDARA ◽  
IVO TOŠEVSKI
Keyword(s):  
New Species ◽  
Morphological Study ◽  
Host Plants ◽  
Molecular Study ◽  
Morphological Characters ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
The Other ◽  
Distribution Data ◽  
Two New Species

Two new species of the weevil genus Rhamphus from Italy are herein described: R. bavierai n. sp. (Sicily) and R. hampsicora n. sp. (Sardinia). Both are morphologically and from a molecular perspective close to R. oxyacanthae (Marsham, 1802) and R. monzinii Pesarini & Diotti, 2012. Aside from a diagnostic description and a synoptic key, distribution data and notes on the host plants of the four species are reported. Whereas R. monzinii can be distinguished by several morphological characters, the other three species are morphologically very similar to each other and separable only by the combination of a few subtle characters. On the contrary, a preliminary molecular study revealed substantial divergences of mtCOI from 6.2 to 14.9% between the species, confirming the importance of an integrative taxonomy.  

Integrative taxonomy of the Asian skinks Sphenomorphus stellatus (Boulenger, 1900) and S. praesignis (Boulenger, 1900) with the resurrection of S. annamiticus (Boettger, 1901) and the description of a new species from Cambodia

Zootaxa ◽  
2019 ◽  
Vol 4683 (3) ◽  
pp. 381-411 ◽  
Author(s):  
L. LEE GRISMER ◽  
PERRY L., JR. WOOD ◽  
EVAN S. H. QUAH ◽  
SHAHRUL ANUAR ◽  
NIKOLAY A. POYARKOV ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Color Pattern ◽  
Integrative Taxonomy ◽  
Peninsular Malaysia ◽  
Wildlife Sanctuary ◽  
Molecular Phylogenetic ◽  
Univariate Analyses ◽  
A New Species

Molecular phylogenetic analyses of the sister species Sphenomorphus stellatus and S. praesignis based on the mitochondrial genes 12S and 16S rRNA recover the former as paraphyletic with respect to the latter in that a specimen of S. stellatus from the type locality in Peninsular Malaysia is more closely related to S. praesignis than to Indochinese populations of S. stellatus. Furthermore, the phylogeny indicates that the Indochinese populations represent two species, thus resulting in four major lineages within this clade. These relationships are consistent with multivariate and univariate analyses of morphological and discrete color pattern data which statistically define and diagnose the four lineages and together with the molecular data, provide the foundation for robust, testable, species-level hypotheses. As such, S. stellatus is herein restricted to Peninsular Malaysia; S. annamiticus is resurrected for the circum-continental populations ranging through southeastern Thailand, southern Cambodia, and southern Vietnam; a new species—S. preylangensis sp. nov.—is described from an isolated mountain, Phnom Chi, from the Prey Lang Wildlife Sanctuary in central Cambodia; and the taxonomy of S. praesignis remains unchanged. The description of S. preylangensis sp. nov. underscores the necessity to conserve this remnant of lowland evergreen rainforest in the Prey Lang Wildlife Sanctuary. 

A new species of Heimioporus (Boletaceae) from southern China

Phytotaxa ◽  
2019 ◽  
Vol 415 (4) ◽  
pp. 179-188
Author(s):  
XIANG-NYU CHEN ◽  
MING ZHANG ◽  
TAI-HUI LI ◽  
NIAN-KAI ZENG
Keyword(s):  
New Species ◽  
Southern China ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Morphological Characters ◽  
Molecular Data ◽  
Translation Elongation ◽  
Distinct Member ◽  
Elongation Factor 1 ◽  
A New Species

Heimioporus sinensis, collected from tropical and subtropical areas of China, is introduced as a new species based on both morphological characters and molecular data. The species is characterized by the purplish red to deep magenta pileus, the reticulated stipe, the irregularly reticulate to reticulate-alveolate basidiospores 11.5–13.5 × 8–9.5 μm, and a trichodermal to intricately trichodermal pileipellis. Phylogenetic analyses based on the nuc 28S rDNA D1-D2 domains (28S) and the translation elongation factor 1-α gene (tef1-α) showed that H. sinensis is a distinct member of the genus Heimioporus in the subfamily Xerocomoideae.

Paris lihengiana (Melanthiaceae: Parideae), a new species from Yunnan, China

Phytotaxa ◽  
2019 ◽  
Vol 392 (1) ◽  
pp. 45 ◽  
Author(s):  
ZHUN XU ◽  
NENG WEI ◽  
YING TAN ◽  
SHUAI PENG ◽  
VERONICAH MUTELE NGUMBAU ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Yunnan Province ◽  
Leaf Surface ◽  
Morphological Characters ◽  
Molecular Data ◽  
Abaxial Leaf Surface ◽  
Molecular Phylogenetic ◽  
Plastid Markers ◽  
A New Species

Paris lihengiana (Melanthiaceae), a new species from Yunnan Province, China, is described and illustrated based on evidence from morphological characters and molecular phylogeny. It differs from other species of Paris in its pubescent stem, pedicel and abaxial leaf surface, as well as other characters. Molecular phylogenetic analysis of 33 taxa in Paris was conducted based on nuclear ribosomal ITS and six plastid markers. Paris lihengiana is supported as a new species by both morphological characters and molecular data.

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