Characterization of gonadotropin-induced oocyte jelly coat from the catfish Heteropneustes fossilis (Bloch)

2019 ◽  
Vol 17 (1) ◽  
pp. 287-294
Author(s):  
A. Mishra ◽  
K. P. Joy
Keyword(s):  
Heteropneustes Fossilis ◽  
Jelly Coat

scholarly journals O-glycan variability of egg-jelly mucins from Xenopus laevis: characterization of four phenotypes that differ by the terminal glycosylation of their mucins

2000 ◽  
Vol 352 (2) ◽  
pp. 449-463 ◽  
Author(s):  
Yann GUERARDEL ◽  
Ossarath KOL ◽  
Emmanuel MAES ◽  
Tony LEFEBVRE ◽  
Bénoni BOILLY ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Molecular Mass ◽  
Electrophoretic Analysis ◽  
High Molecular Mass ◽  
Methylation Analysis ◽  
Glycosylation Pattern ◽  
Jelly Coat ◽  
New Family ◽  
Egg Jelly

Eggs from Xenopus laevis are surrounded by several layers of jelly that are needed for proper fertilization. Jelly coat is composed of high-molecular-mass glycoconjugates to which are bound many globular proteins. O-glycans released from the jelly coat of X. laevis have been partially described in previous studies. In this study, we compared the glycosylation pattern of the egg jelly coat isolated from six specimens of X. laevis. The O-glycans were released from jelly coats by alkali/borohydride treatment. Structural characterization was performed through a combination of one- and two-dimensional 1H-NMR and methylation analysis. This allowed the description of a new family of sulphated O-glycans present in jelly coats of all X. laevis. However, the jelly O-glycans showed a low extent of polymorphism between specimens. This intra-specific variability was restricted to the terminal substitution of O-linked oligosaccharides. The differential expression of two glycosyltransferase [an α-(1 → 4) galactosyltransferase and an α-(1 → 3) fucosyltransferase] activities resulted in the characterization of four phenotypes of X. laevis. Furthermore, electrophoretic analysis suggested that the high-molecular-mass fraction of jelly coat was mostly composed of mucin-type glycoproteins. Blot analysis with lectins confirmed that the glycan variability was borne by these mucin-type components. However, fertilization assays suggested that the glycan polymorphism had no repercussion on egg fertilizability.

Characterization of Lex , Ley and A Ley antigen determinants in KDN-containing O -linked glycan chains from Pleurodeles waltlii jelly coat eggs

FEBS Letters ◽  
1992 ◽  
Vol 298 (1) ◽  
pp. 39-43 ◽  
Author(s):  
Gérard Strecker ◽  
Jean-Michel Wieruszeski ◽  
Jean-Claude Michalski ◽  
Catherine Alonso ◽  
Benoni Boilly ◽  
...  
Keyword(s):  
Jelly Coat ◽  
Pleurodeles Waltlii ◽  
Ley Antigen

Characterization of MboI satellites in Cirrhina mrigala and Clarias batrachus (Pisces)

Genome ◽  
1998 ◽  
Vol 41 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Bhaja K Padhi ◽  
Sudip K Ghosh ◽  
Radha K Mandal
Keyword(s):  
Parental Species ◽  
Labeo Rohita ◽  
Clarias Gariepinus ◽  
Clarias Batrachus ◽  
Catla Catla ◽  
Genetic Introgression ◽  
Heteropneustes Fossilis ◽  
Reciprocal Hybrids ◽  
Species Specific

We have cloned and characterized two highly reiterated, tandemly repeated, and A + T rich MboI DNA fragments, one in Cirrhina mrigala (Cyprinidae), with a monomer size of 266 bp, and one in Clarias batrachus (Clariidae), with a monomer size of 227 bp. The MboI fragment in C. mrigala is species-specific and absent in other carps, such as Catla catla and Labeo rohita. The MboI fragment in C. batrachus was also present in two other catfishes tested, namely Clarias gariepinus and Heteropneustes fossilis. In C. mrigala xC. catla and C. mrigala x L. rohita hybrids, the C. mrigala specific MboI fragment is inherited uniparentally. In the reciprocal hybrids of C. batrachus xH. fossilis, the satellite ladder contains the bands of both parental species. The MboI satellite of carp may be useful in genetic introgression analysis and that of catfish in distinguishing between gynogenetic progeny and true hybrids.

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