Kajian Predasi Catfish terhadap Larva Nyamuk Aedes aegypti Sebagai Upaya Pencegahan Penyebaran Demam Berdarah Dengue

Aedes aegypti acts as a primary vector of the dengue virus which causes Indonesia to become a dengue-endemic country. Chemical and physical vector control methods to prevent the spread of DHF are considered less effective so that additional biological vector control is needed. The purpose of this paper is to examine the potential ability of catfish predation as Ae. aegypti vector controller. This paper was prepared by reviewing eight Indonesian research journals, eight international research journals, reports and regulations of the Ministry of Health of the Republic of Indonesia, the website of the World Health Organization (WHO), the Centers for Disease Control and Prevention (CDC), the University of Florida Entomology and Nematology, and one textbook. The results showed that the predation ability of African sharptooth catfish (Clarias garipenus) was 80.3% greater on average than Cyprinus carpio, Ctenopharyngodon idella, and Oreochromis niloticus as well as the predation ability of Walking catfish (Clarias batrachus) with an average of 79.6%. larger than Anabas testudineus, Oreochromis niloticus, and Poecilia reticulata. Further experimental research to deepen scientific information on the predation ability of various catfish species against Ae. aegypti mosquito larvae are still needed. Cooperation between fields and communities is also needed to prevent the spread of dengue disease properly. Mosquito larvae eating fish belonging to catfish species, namely African sharptooth catfish (Clarias garipenus) and walking catfish (Clarias batrachus) have the potential to be a new biological vector controller because they have very high predation power compared to other mosquito larvae eating fish.

Chronic Effects of Diazinon® Exposures Using Integrated Biomarker Responses in Freshwater Walking Catfish, Clarias batrachus

Diazinon exposures have been linked to the onset of toxic pathways and adverse outcomes in aquatic species, but the ecological implications on model species are not widely emphasized. The objective of this study was to determine how the organophosphate pesticide diazinon affected hematological (hemoglobin, total red blood count, total white blood count, and mean corpuscular hemoglobin), growth (condition factor, hepatosomatic index, specific growth rate), biochemical (total serum glucose, total serum protein), and endocrine (growth hormone, tri-iodothyronine, and thyroxine) parameters in Clarias batrachus after chronic exposure. Diazinon was administered at predefined exposure doses (0.64 and 1.28 mg/L) and monitored at 15, 30, and 45 days into the investigation. Observation for most biomarkers revealed patterns of decreasing values with increasing toxicant concentration and exposure duration. Correlation analysis highlighted a significant inverse relationship between variables (mean corpuscular hemoglobin, condition factor, specific growth rate, tri-iodothyronine, thyroxine, and total serum protein) and elevated chronic diazinon exposure concentrations. The integrated indices (IBR and BRI) indexes were used to provide visual and understandable depictions of toxicity effects and emphasized the relativity of biomarkers in terms of sensitivity and magnitude or severity of responses under graded toxicant exposures. The significant damage reflected by evaluated parameters in diazinon exposure groups compared to control portends risks to the health of local fish populations, including Clarias batrachus in aquatic systems adjacent to agrarian landscapes.

The Effectiveness of Turmeric Enriched Pellets in Curing the Gill of Clarias batrachus Infected with Aeromonas hydrophila

Curcumin is an antibiotic agent extracted from Curcuma longa. To understand the effectiveness of curcumin in curing the gill of Clarias batrachus infected with Aeromonas hydrophila, a study was conducted from August to November 2020. There were 3 treatments applied, namely turmeric enriched pellets 0.7g/kg (P1); 0.9g/kg (P2) and 1.1g/kg (P3). Prior to the treatment, the fish was infected with A. hydrophila (0.1 m of A. hydrophila culture, 1.0x108cells/ml). The infected fish was kept for 6 days until the clinical signs of MAS disease occurred. By the 7th day, the fish was feed with turmeric enriched pellets. For negative control (Cn) the fish was not infected with A. hydrophila nor treated with turmeric, while the positive control (Cp) was infected, but no turmeric treatment. The fish was reared for 30 days and by the end of the experiment, the gill was removed and processed for histological study (5L sliced and HE stained). Result indicated that the gill of the negative control fish was normal, while that of the positive control shown abnormalities such as hemorrhages, necrotic cells, fused lamellae and enlarged cartilaginous cells. The turmeric treated fish shown less damage in the gill. The best results were obtained in C3, as the gill structure was almost normal with slightly hemorrhage. In the C1 and C2 treated fishes, the gill shown light abnormality such as fused lamella and hemorrhage. Data obtained shown that the consumption of turmeric enriched pellet was effective to cure the A. hydrophyla infected gill.

Optimization of Hormone for Artificial Breeding and Seed Production of Clarias batrachus (Linnaeus, 1758) Under Captive Condition

Aims: The study aimed to develop brood stock of Magur (Clarias batrachus), artificial breeding and seed production under captive condition using growth hormones in north-eastern Bangladesh. Study Design: Fifteen pairs of brood Clarias batrachus were used in this experiment. Brood fishes were reared up to maturation for spawning operation in the farm by providing artificial diet for 4-5 months before onset of breeding season. In the present experiment, the brood fishes were induced with three growth hormones viz., Ovupin (100 mg dompridone and 0.2 mg S-GnRHa) at the rate of 1 ml.kg-1 body weight, Flash (20 mg S-GnRHa, 10 mg dompridone IP and propylene glycol) at the rate of 1 ml.kg-1 body weight and Human Chorionic Gonadotropin (HCG) hormone at the rate of 2272 IU.kg-1 body weight; respectively. The broods were injected with single injection in all treatments. Place and Duration of Study: The present study was conducted on induced spawning of Magur (Clarias batrachus) at the Reliance Aqua Farm, Boilor, Trisal of Mymensingh district from March to August, 2016. Methodology: Sexually mature healthy, uninjured male and female fishes were collected from the broodstock ponds for induced breeding. The fishes were transferred in the clean and germ free conditioning tanks for about 6-7 hours. During conditioning, continuous splash of water was given by keeping the male and female separate. The fishes were then weighed individually and injected intramuscularly with Ovupin, Flash and HCG hormone respectively. Only females were injected at 45° angle of the caudal peduncle. About 23-24 hours of post-injection the testes from males were dissected and mixed with stripping eggs for fertilization. Results: The incubation period for fertilized eggs were 23-24 hours for all the treatments. Fertilization rates were 78.20±0.52, 93.09±0.61 and 81.60±0.97 % and hatching rates were 54.93±0.61, 75.77±0.47 and 64.41±0.71 % in Ovupin, Flash and HCG treatments, respectively. Conclusion: This study revealed higher fertilization (93.09 %) and hatching (75.77 %) rate was achieved with Flash hormone. Therefore, Flash hormone could be effective for the artificial breeding and seed production of Magur (Clarias batrachus).

Parasites prevalence which infecting freshwater fishes in Mulur Reservoir of Sukoharjo District, Indonesia

Iriansyah AH, Budiharjo A, Sugiyarto. 2020. Parasites prevalence infecting freshwater fishes in Mulur Reservoir of Sukoharjo District, Indonesia. Bonorowo Wetlands 10: 66-71. Mulur Reservoir is one of the natural fish habitats and consumption fish cultivation in Sukoharjo District, Indonesia. Excessive use of the reservoir area causes the decreasing of water quality which affects fish life sustainability, one of them can cause the fish susceptibility to infection by parasites. The purpose of this research was to identify the types of parasites which infect on consumption of fish in Mulur Reservoir and calculate the prevalence value. Samples were taken by purposive sampling for gourami (Osphronemus gouramy), tilapia (Oreochromis niloticus), betutu fish (Oxyeleotris marmorata), catfish (Clarias batrachus), and jambal (Pangasius djambal) 10 fishes for each type. The parts of fish infected by ectoparasites such as body mucus, fin mucus, head mucus, and gills were taken by scrapping and observed under a microscope with magnification between 100-400x. The results showed that 5 types of ectoparasites were Epistylis sp., Ichthyophthirius multifilis, Trichodina sp., Dactylogyrus sp., and Gyrodactylus sp. with an average prevalence of more than 50%. There are more ectoparasites in domesticated fish than in wild fish.