Multiplex PCR for simultaneous identification of turkey, ostrich, chicken, and duck

Differentiation between Bursaphelenchus xylophilus and other related, non-pathogenic species can be ambiguous when based exclusively on morphological characters. The morphology of B. mucronatus and B. fraudulentus most closely resembles that of B. xylophilus. Moreover, all of these nematodes are found in both Asia and Europe and can colonise various species of pine. Therefore, for phytosanitary purposes it is necessary to identify the three species precisely and rapidly. We report the results of a multiplex PCR that utilises four primers to identify and discriminate the three Bursaphelenchus species simultaneously. The multiplex PCR yielded DNA fragments of 767, 305 and 132 bp, for B. xylophilus, B. mucronatus and B. fraudulentus, respectively. This primer combination has produced reliable results in multiplex PCR assays with a number of different populations of the listed species, and no cross-reactions were observed with other Bursaphelenchus species. The described approach is simple, reliable and cheaper than other molecular methods presently used for simultaneous identification of the above three species within the xylophilus group.

Download Full-text

Novel Molecular Assay for Simultaneous Identification of Neglected Lungworms and Heartworms Affecting Cats

Journal of Clinical Microbiology ◽

10.1128/jcm.00901-15 ◽

2015 ◽

Vol 53 (9) ◽

pp. 3009-3013 ◽

Cited By ~ 23

Author(s):

Angela Di Cesare ◽

Fabrizia Veronesi ◽

Antonio Frangipane di Regalbono ◽

Raffaella Iorio ◽

Donato Traversa

Keyword(s):

Multiplex Pcr ◽

Scientific Community ◽

Clinical Impact ◽

Molecular Assay ◽

Aelurostrongylus Abstrusus ◽

Troglostrongylus Brevior ◽

Geographical Expansion ◽

Angiostrongylus Chabaudi ◽

Simultaneous Identification

Feline lungworms and heartworms are stimulating the interest of the scientific community due to their clinical impact and apparent geographical expansion. Diagnosis of the infections caused by these nematodes is indeed challenging. This report describes a novel multiplex PCR able to identify simultaneously three species of lungworms (Aelurostrongylus abstrususandTroglostrongylus brevior) and heartworms (Angiostrongylus chabaudi) affecting felids. Epidemiological and clinical perspectives are discussed.

Download Full-text

Rapid and Simultaneous Identification of Body Parts from the Morphologically Similar Sharks Carcharhinus obscurus and Carcharhinus plumbeus (Carcharhinidae) Using Multiplex PCR

Marine Biotechnology ◽

10.1007/s101260000071 ◽

2001 ◽

Vol 3 (3) ◽

pp. 231-240 ◽

Cited By ~ 53

Author(s):

Melissa Pank ◽

Michael Stanhope ◽

Lisa Natanson ◽

Nancy Kohler ◽

Mahmood Shivji

Keyword(s):

Multiplex Pcr ◽

Body Parts ◽

Carcharhinus Plumbeus ◽

Carcharhinus Obscurus ◽

Simultaneous Identification

Download Full-text

cpn60 Gene-Based Multiplex-PCR Assay for Simultaneous Identification of Streptococcal Species

Acta Veterinaria Brno ◽

10.2754/avb200675020235 ◽

2006 ◽

Vol 75 (2) ◽

pp. 235-240 ◽

Cited By ~ 7

Author(s):

A. Dmitriev ◽

M. Bhide ◽

I. Mikula

Keyword(s):

Multiplex Pcr ◽

Streptococcus Agalactiae ◽

Streptococcus Dysgalactiae ◽

Pcr Assay ◽

Streptococcus Uberis ◽

Multiplex Pcr Assay ◽

Streptococcal Species ◽

Species Specific ◽

Simultaneous Identification

The cpn60 genes of Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis were sequenced and a certain polymorphism of cpn60 genes was revealed. Presumable species-specific pairs of primers were designed and their specificity was confirmed by PCR. Based on these data, the cpn60 gene-based multiplex-PCR assay was developed. It was found to be effective for simultaneous identification of S. agalactiae, S. dysgalactiae and S. uberis strains.

Download Full-text

Identification of Powdery Mildew Blumeria graminis f. sp. tritici Resistance Genes in Selected Wheat Varieties and Development of Multiplex PCR

Open Chemistry ◽

10.1515/chem-2019-0024 ◽

2019 ◽

Vol 17 (1) ◽

pp. 157-165 ◽

Cited By ~ 3

Author(s):

Agnieszka Tomkowiak ◽

Roksana Skowrońska ◽

Dorota Weigt ◽

Michał Kwiatek ◽

Jerzy Nawracała ◽

...

Keyword(s):

Multiplex Pcr ◽

Plant Material ◽

Block Design ◽

Life Sciences ◽

Gene Identification ◽

Reaction Conditions ◽

Wheat Varieties ◽

Randomized Complete Block Design ◽

Simultaneous Identification ◽

Pcr Conditions

AbstractThe aim of the study was to identify the Pm2, Pm3a, Pm4b and Pm6 genes and to develop multiplex PCR reaction conditions to reduce time and limit analysis costs. The following molecular markers were used for gene identification: Xcfd81, Whs350 and Xgwm205 (for Pm2), Pm3a (for Pm3a), STS_241 and Xgwm382 (for Pm4b), NAU/BCDSTS 135-2 (for Pm6). Plant material consisted of 7 popular European wheat varieties from the wheat collection at the Department of Genetics and Plant Breeding of the Poznań University of Life Sciences. The field experiment was established in 2017 and 2018 on 10 m2 plots in a randomized complete block design in three replicates in the Dłoń Agricultural Experimental Farm of the Poznań University of Life Sciences (51°41’23.835”N 017°4’1.414”E). The analyses demonstrated that the accumulation of all identified Pm genes was found in the Assosan variety. The accumulation of the Pm2, Pm4b and Pm6 genes was found in Atomic, Bussard, Lear, Sparta, Tonacja and Ulka varieties. The work also involved developing multiplex PCR conditions for Xcfd81 and STS_241 and Xcfd81 and Xgwm382 primer pairs, allowing the simultaneous identification of the Pm2 and Pm4b genes.

Download Full-text