Field Evaluation and Genetic Stability Assessment of Regenerated Plants Produced Via Direct Shoot Organogenesis from Leaf Explant of an Endangered ‘Asthma Plant’ (Tylophora indica) Along with Their In Vitro Conservation

2013 ◽  
Vol 36 (5) ◽  
pp. 551-562 ◽  
Author(s):  
Sk. Moquammel Haque ◽  
Biswajit Ghosh
Keyword(s):  
Genetic Stability ◽  
Shoot Organogenesis ◽  
Field Evaluation ◽  
Leaf Explant ◽  
Stability Assessment ◽  
Tylophora Indica ◽  
Regenerated Plants ◽  
Direct Shoot Organogenesis ◽  
Direct Shoot

scholarly journals Direct Shoot Organogenesis of Medicago sativa

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 628f-628
Author(s):  
Guochen Yang ◽  
Marihelen Kamp-Glass
Keyword(s):  
Genetic Transformation ◽  
Shoot Regeneration ◽  
Shoot Organogenesis ◽  
Multiple Shoots ◽  
Direct Shoot Regeneration ◽  
Callus Initiation ◽  
Direct Shoot Organogenesis ◽  
Direct Shoot ◽  
In Vitro Shoot Regeneration

An efficient and reliable protocol of in vitro shoot regeneration must be first established to have a successful genetic transformation. As a member of legume family, alfalfa is very difficult for direct shoot regeneration. There is no published information on direct shoot organogenesis, although success has been well documented on embryogenesis, which must go through callus stage. Different plant growth regulators at various concentrations were evaluated for callus initiation, development, and direct shoot regeneration. Multiple shoots were produced directly from each individual explant. This will provide an efficient means for production of transgenic alfalfa plants. Therefore, genetic transformation of Medicago germplasm will be significantly expedited.

scholarly journals Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Catalina Restrepo-Osorio ◽  
Alejandro Gil-Correal ◽  
Lina Chamorro-Gutiérrez ◽  
Viviana Ramírez-Ríos ◽  
Javier C. Álvarez ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Shoot Organogenesis ◽  
Plukenetia Volubilis ◽  
Sacha Inchi ◽  
Direct Shoot Organogenesis ◽  
Direct Shoot

scholarly journals High Efficiency Direct Shoot Organogenesis from Leaf Segments ofAerva lanata(L.) Juss. Ex Schult by Using Thidiazuron

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
K. Varutharaju ◽  
C. Soundar Raju ◽  
C. Thilip ◽  
A. Aslam ◽  
A. Shajahan
Keyword(s):  
Shoot Organogenesis ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Naphthaleneacetic Acid ◽  
Scale Production ◽  
Ms Medium ◽  
Leaf Segments ◽  
Direct Shoot Organogenesis ◽  
Direct Shoot

An efficient protocol for direct shoot organogenesis has been developed for the medicinal plantAerva lanata(L.) Juss. ex Schult. Regeneration was achieved from leaf segments of 20 days oldin vitroplantlets raised on Murashige and Skoog (MS) medium containing 0.25–2.0 mg L−1thiadiazuron (TDZ), 3% sucrose, and 0.8% agar. After 21 days of culture incubation, maximum number of shoot organogenesis (23.6 ± 0.16) was obtained on medium containing 1.0 mg L−1TDZ. The shoots were able to producein vitroflowers on medium containing 1.0 mg L−1TDZ in combination with 0.25–0.5 mg L−1  α-naphthaleneacetic acid (NAA). Histological observation showed that the epidermal cells of the leaf explants exhibited continuous cell division led to formation of numerous dome shaped meristematic protrusions and subsequently developed into adventitious shoots. Upon transfer of shootlets to half strength MS medium containing 1.0 mg L−1indole-3-butyric acid (IBA), around 86% of the regenerated shoots formed roots and plantlets. Rooted plants were hardened and successfully established in the soil at the survival rate of 92%. The regeneration protocol developed in this study provides an important method of micropropagation of this plant. Furthermore, this protocol may be used for a large scale production of its medicinally active compounds and genetic transformations for further improvement.

scholarly journals 081 Genetic Analysis of Direct Shoot Organogenesis on Hypocotyls of Antirrhinum majus L.

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 455B-455
Author(s):  
Monica E. Figueroa-Cabanas ◽  
Dennis P. Stimart
Keyword(s):  
Apical Meristem ◽  
Culture Medium ◽  
Shoot Organogenesis ◽  
Adventitious Shoot ◽  
Antirrhinum Majus ◽  
Optimal Conditions ◽  
Culture Duration ◽  
Direct Shoot Organogenesis ◽  
Direct Shoot

Direct shoot organogenesis (DSO) on Antirrhinum majus L. (snapdragon) was evaluated in vitro to determine the inheritance of genes conditioning this response. One-centimeter-long hypocotyls excised from 2-week-old seedlings started in vitro in the dark on Murashige and Skoog medium served as explants. Optimal conditions for DSO on explants included hypocotyl excision from 10-day-old seedlings, 2.22 μmol BA in the culture medium, and a 21-day culture duration. An adventitious shoot was counted once it developed a stem terminated by at least one leaf appearing to have originated from an apical meristem. Seven populations were evaluated for DSO: parent 1 (P1) with lowest DSO (0.3 shoots); parent 2 (P2) with highest DSO (13.9 shoots); F1 (P1 × P2); F1 (P2 × P1); F2 (self-pollination of F1); P1 × [P1 × P2]; and P2 × [P1 × P2]. P1 and P2 were chosen as parents based on DSO counts being lowest and highest, respectively, of inbreds evaluated. DSO appears to be a trait under nuclear genetic control. High DSO appears to be dominant over low DSO. The trait appears to be simply inherited through one or two genes.

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