In Vitro Multiple Shoot Induction and Plant Regeneration from Male Ephedra foliata: A Potential Medicinal Gymnosperm

2018 ◽  
Vol 42 (3) ◽  
pp. 271-276
Author(s):  
Akshay Joshi ◽  
Subhash Deokule
2010 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M. F. Hasan ◽  
B. Sikdar

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)


2017 ◽  
Vol 23 (1) ◽  
Author(s):  
PUSHPLATA DODAKE ◽  
MALIKA PAL

A competent protocol was developed for in vitro plant regeneration and multiplication through callus culture of Eclipta alba. Perfect medium for callus establishment through nodal tissue was observed in 2,4-D supplemented media. The callus formed was creamish yellow in colour, showed a growth period of three to four weeks. A moderate quality callus was obtained with different gradients of BAP supplemented medium. A white to creamy callus with rapid growth was observed in the medium supplemented with NAA. Callus was subjected for multiple shoot induction.


2011 ◽  
Vol 10 (51) ◽  
pp. 10509-10515 ◽  
Author(s):  
H Zhang Y ◽  
F Gao S ◽  
Du T ◽  
G Chen H ◽  
H Z Wang ◽  
...  

2010 ◽  
Vol 105 (2) ◽  
pp. 211-217 ◽  
Author(s):  
Patricia Corral ◽  
Rubén Mallón ◽  
Juan Rodríguez-Oubiña ◽  
María Luz González

2012 ◽  
Vol 39 (2) ◽  
pp. 114-120 ◽  
Author(s):  
Kee-Hwa Bae ◽  
Myung-Suk Ko ◽  
Nam-Young Kim ◽  
Jae-Mo Song ◽  
Gwan-Pil Song

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