Effects of Nitrogen Rates on the Productivity and Nutritive Value of Forage Grass Grown under Extreme Climatic Conditions

This vegetative experiment was carried out at the greenhouse of Vytautas Magnus University Agriculture Academy Open Access Joint Research Centre of Agriculture and Forestry (Lithuania) in 2020–2021. The aim of these studies was to determine the effect of different nitrogen rates on the productivity and nutritional quality of forage grasses (a mixture of red clover and timothy) under the most common extremes of climate change, i.e., soil moisture deficiency and surplus. Under drought and waterlogging stresses, fertilization of the red clover and timothy mixture with high N rates was ineffective. The clover and timothy mixture recovery after drought took 21 days. The aboveground dry biomass of the clover and timothy mixture grown under drought conditions was significantly lower by 36.3 to 47.2% compared to that formed under optimum soil moisture and waterlogging conditions. The root biomass of forage grass mixtures was lowest under drought conditions when fertilized at the highest N rate (N25+120). The aboveground biomass of clover grown under different soil moisture conditions depended on the number of plants (r2 = 0.78, p < 0.01) and assimilating leaf area (r2 = 0.83, p < 0.01), and that of timothy on the number of vegetative tillers (r2 = 0.46, p < 0.05). Under drought simulation conditions, increasing the N rate increased the crude protein and crude fibre contents in the aboveground biomass of the clover and timothy mixture, while the crude ash content decreased.

Complex polyploid and hybrid species in an apomictic and sexual tropical forage grass group: genomic composition and evolution in Urochloa (Brachiaria) species

Background and Aims Diploid and polyploid Urochloa (including Brachiaria, Panicum and Megathyrsus species) C4 tropical forage grasses originating from Africa are important for food security and the environment​, often being planted in marginal lands worldwide. We aimed to characterize the nature of their genomes, the repetitive DNA, and the genome composition of polyploids, leading to a model of the evolutionary pathways within the group including many apomictic species. Methods Some 362 forage grass accessions from international germplasm collections were studied, and ploidy determined using an optimized flow cytometry method. Whole-genome survey sequencing and molecular cytogenetic analysis were used to identify chromosomes and genomes in Urochloa accessions belonging to the 'brizantha' and 'humidicola' agamic complexes and U. maxima. Key Results Genome structures are complex and variable, with multiple ploidies and genome compositions within the species, and no clear geographical patterns. Sequence analysis of nine diploid and polyploid accessions enabled identification of abundant genome-specific repetitive DNA motifs​. In situ hybridization with a combination of repetitive DNA and genomic DNA probes, identified evolutionary divergence and allowed us to discriminate the different genomes present in polyploids. Conclusions We suggest a new coherent nomenclature for the genomes present​. We develop a model of evolution at the whole-genome level in diploid and polyploid accessions showing processes of grass evolution. We support the retention of narrow species concepts for U. brizantha, U. decumbens, and U. ruziziensis, and do not consider diploids and polyploids of single species as cytotypes. The results and model will be valuable in making rational choices of parents for new hybrids, assist in use of the germplasm for breeding and selection of Urochloa with improved sustainability and agronomic potential, and will assist in measuring and conserving biodiversity in grasslands.

Contribution of Awns to Seed Yield and Seed Shattering in Siberian Wildrye Grown under Irrigated and Rainfed Environments

The seed yield of grass species is greatly dependent on inflorescence morphological traits, starting with spikelets per inflorescence and seeds per spikelet, to kernel size, and then to awns. Previous studies have attempted to estimate the contribution of these traits on the harvested yield of major cereal crops, but little information can be accessed on the influence of awns on seed yield of forage grass species. Siberian wildrye (Elymus sibiricus L.) is a widely important perennial forage grass used to increase forage production in arid and semi-arid grasslands. The grass has long inflorescences with long awns developed at the tip end of the lemmas in the florets. In order to evaluate the effect of awns on Siberian wildrye seed production, awn excision analyses from 10 accessions were performed at flowering stage under irrigated and rainfed regimes. Overall, awn excision reduced thousand-seed weight and seed size under both irrigated and rainfed regimes, which decreased final seed yield per plant. De-awned plants produced significantly more seeds per inflorescence, but spikelets per inflorescence was not influenced by awn excision in either condition. Moreover, histological analyses showed a high degradation of the abscission layer in the awned plants than de-awned ones, and awn excision evidently improved average seed breaking tensile strength (BTS), and thus decreased the degree of seed shattering. In conclusion, the observed significant impact of awn excision on different yield-related traits mirrored the impact of awns on the performance of Siberian wildrye under diverse growing conditions. These results provide useful information for plant breeders, seed producers, and researchers to efficiently improve seed production in Siberian wildrye.

Application of SCoT markers for accessing of genetic diversity of gramineous forage grass species

Using the SCoT marker system, 8 varieties of cereal grasses belonging to 5 species were analyzed: Festuca pratensis, Lolium perenne, Lolium multiflorum, Festuca rubra, Festulolium. Of the 10 tested SCoT markers, 7 informative markers were selected that reveal interspecies genetic polymorphism. According to the results of the analysis, DNA profiles characteristic of each studied species were obtained, and primers allowing to detect intervarietal differences for subsequent identification and molecular genetic passportization were selected.