High frequency regeneration in soybean [Glycine max (L.) Merrill.] through direct somatic embryogenesis from immature cotyledons

2015 ◽  
Vol 20 (3) ◽  
pp. 232-239 ◽  
Author(s):  
H. N. Huynh ◽  
S. K. Lal ◽  
S. K. Singh ◽  
K. V. Prabhu ◽  
A. Talukdar
Keyword(s):  
Somatic Embryogenesis ◽  
Glycine Max ◽  
High Frequency ◽  
Direct Somatic Embryogenesis ◽  
Immature Cotyledons ◽  
Glycine Max L

Regeneration of soybean (Glycine max L. Merrill) through direct somatic embryogenesis from the immature embryonic shoot tip

2010 ◽  
Vol 46 (3) ◽  
pp. 265-273 ◽  
Author(s):  
Murugan Loganathan ◽  
Subbiyan Maruthasalam ◽  
Ling Yin Shiu ◽  
Wei Ching Lien ◽  
Wen Hwei Hsu ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Glycine Max ◽  
Shoot Tip ◽  
Direct Somatic Embryogenesis ◽  
Glycine Max L

scholarly journals Somatic embryogenesis from immature cotyledons of soybean (Glycine max (L.) Merr.): ontogeny of somatic embryos

2006 ◽  
Vol 49 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Karla Galvão Bezerra dos Santos ◽  
Jorge Ernesto de Araújo Mariath ◽  
Maria Cecília C. Moço ◽  
Maria Helena Bodanese-Zanettini
Keyword(s):  
Somatic Embryogenesis ◽  
Glycine Max ◽  
Somatic Embryos ◽  
Zygotic Embryos ◽  
Immature Zygotic Embryos ◽  
Immature Cotyledons ◽  
Glycine Max L

Soybean somatic embryos obtained from immature zygotic embryos were histologically analyzed in order to determine possible ontogenetic routes followed by these embryos. The proembryo stage and globular, heart-shaped, torpedo and cotyledonary embryo stages were found, closely resembling the ontogeny of zygotic embryos. However, the absence of a characteristic suspensor as well as the delay in the establishment of inner organization were the main differences between these two embryogenic processes.

High-frequency direct somatic embryogenesis in thin layer cultures of hybrid seed geranium (Pelargonium ×hortorum)

1993 ◽  
Vol 71 (3) ◽  
pp. 408-413 ◽  
Author(s):  
Ravinder Gill ◽  
Jean M. Gerrath ◽  
Praveen K. Saxena
Keyword(s):  
Somatic Embryogenesis ◽  
Thin Layer ◽  
High Frequency ◽  
Somatic Embryos ◽  
Indoleacetic Acid ◽  
Thin Layers ◽  
Direct Somatic Embryogenesis ◽  
Induction Medium ◽  
Culture Initiation ◽  
Regeneration Potential

Thin layer explants from the hypocotyls of young seedlings of geranium (Pelargonium ×hortorum Bailey) produced somatic embryos in response to thidiazuron (TDZ) or a combination of indoleacetic acid and N6-benzylaminopurine, but the number of embryos was much less in the latter than with TDZ. The development of somatic embryos in cultures of thin layers was rapid and the number of embryos was about eightfold higher than in cultures of whole hypocotyl explants. Thin layer explants of 1-week-old seedlings produced a high number of somatic embryos than those obtained from older seedlings. The cultivar Scarlet Orbit Improved showed better regeneration potential than 'Sprinter Scarlet'. There were no visible differences in total number of somatic embryos produced in the medium containing sucrose or glucose. The addition of glutamine to the induction medium further improved the number of somatic embryos that developed. Regenerated somatic embryos developed into complete plantlets within 6 to 8 weeks of culture initiation. Key words: Pelargonium ×hortorum, geranium, somatic embryogenesis, thin layers, thidiazuron.

In vitro Culture of Immature Cotyledons of Soya Bean (Glycine max L. Merr.)

1977 ◽  
Vol 41 (1) ◽  
pp. 29-39 ◽  
Author(s):  
J. F. THOMPSON ◽  
J. T. MADISON ◽  
ANNA-MARIA E. MUENSTER
Keyword(s):  
Glycine Max ◽  
In Vitro Culture ◽  
Soya Bean ◽  
Immature Cotyledons ◽  
Glycine Max L
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