In vivo testing of antagonistic fungi against Alternaria brassicicola causing Chinese kale black spot disease

2020 ◽  
Author(s):  
Atima Komhorm ◽  
Suttipong Thongmee ◽  
Todsawat Thammakun ◽  
Thanaprasong Oiuphisittraiwat ◽  
Arom Jantasorn
Keyword(s):  
Black Spot ◽  
Alternaria Brassicicola ◽  
Spot Disease ◽  
Antagonistic Fungi ◽  
Chinese Kale ◽  
In Vivo Testing ◽  
Black Spot Disease

scholarly journals Postharvest Black Spot Disease in Broccoli Caused by Alternaria brassicicola

2009 ◽  
Vol 51 ◽  
pp. 11-15 ◽  
Author(s):  
Yi Liu ◽  
Zhen Xu ◽  
Pinkuan Zhu ◽  
Yan Liu ◽  
Zaibao Zhang ◽  
...  
Keyword(s):  
Black Spot ◽  
Alternaria Brassicicola ◽  
Spot Disease ◽  
Black Spot Disease

scholarly journals Complexity of Brassica Oleracea-Alternaria Brassicicola Susceptible Interaction Reveals Down-regulation of Photosynthesis at Ultrastructural, Transcriptional and Physiological Levels

2020 ◽  
Author(s):  
Violetta Katarzyna Macioszek ◽  
Magdalena Gapińska ◽  
Agnieszka Zmienko ◽  
Mirosław Sobczak ◽  
Andrzej Skoczowski ◽  
...  
Keyword(s):  
Host Cell ◽  
Black Spot ◽  
Epidermal Cells ◽  
Alternaria Brassicicola ◽  
Chloroplast Envelope ◽  
Carotenoid Content ◽  
Down Regulation ◽  
Spot Disease ◽  
Regulation Of Photosynthesis ◽  
Black Spot Disease

Abstract BackgroundBlack spot disease, caused by Alternaria brassicicola in Brassica species, is one of the most devastating diseases all over the world, especially since there is no known fully resistant Brassica cultivar. In this study, the visualization of black spot disease development on B. oleracea var. capitata f. alba (white cabbage) leaves and subsequent ultrastructural, molecular and physiological investigations were conducted. ResultsFollowing germination on B. oleracea leaves, A. brassicicola invaded the host epidermal cells through appressoria, stomata or directly. The fungal attempts to penetrate epidermal cells usually evoked host cell death, and defense reactions manifested by the bright 'halo' around penetration site and host cell wall fortification were also observed. Inter- and intracellular hyphae growth within leaf tissues led to the loss of host cell integrity and various levels of organelle disintegration. Severe symptoms of chloroplast damage included the degeneration of chloroplast envelope and grana, and the loss of electron denseness by stroma at the advanced stage of infection. Transcriptional profiling of infected leaves revealed that photosynthesis was the most negatively regulated biological process. The down-regulation of 6 photosynthesis-related genes involved in light reactions and the Calvin cycle began as early as 12 hours post-inoculation (hpi) and extended to 44 genes at 48 hpi. Although in infected leaves, chlorophyll and carotenoid content did not decrease until 48 hpi, several chlorophyll a fluorescence parameters, such as photosystem II quantum yield (Fv/Fm), non-photochemical quenching (NPQ) or plant vitality parameter (Rdf) decreased significantly at 24 and 48 hpi compared to control leaves. ConclusionsOur results indicate that the initial stages of interaction between B. oleracea and A. brassicicola are not uniform within an inoculation site and show a complexity of host responses and fungal attempts to overcome host cell defense mechanisms. The down-regulation of photosynthesis at the early stage of this susceptible interaction suggests that it may be a part of a host defense strategy, or alternatively, that chloroplasts are targets for the unknown virulence factor(s) of A. brassicicola. However, the observed decrease of photosynthetic efficiency at the later stages of infection is a result of the fungus-induced necrotic lesion expansion.

scholarly journals Complexity of Brassica oleracea–Alternaria brassicicola Susceptible Interaction Reveals Downregulation of Photosynthesis at Ultrastructural, Transcriptional, and Physiological Levels

Cells ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 2329
Author(s):  
Violetta Katarzyna Macioszek ◽  
Magdalena Gapińska ◽  
Agnieszka Zmienko ◽  
Mirosław Sobczak ◽  
Andrzej Skoczowski ◽  
...  
Keyword(s):  
Host Cell ◽  
Brassica Oleracea ◽  
Black Spot ◽  
Alternaria Brassicicola ◽  
Chloroplast Envelope ◽  
Carotenoid Content ◽  
Photochemical Quenching ◽  
Spot Disease ◽  
Cell Defense ◽  
Black Spot Disease

Black spot disease, caused by Alternaria brassicicola in Brassica species, is one of the most devastating diseases all over the world, especially since there is no known fully resistant Brassica cultivar. In this study, the visualization of black spot disease development on Brassica oleracea var. capitata f. alba (white cabbage) leaves and subsequent ultrastructural, molecular and physiological investigations were conducted. Inter- and intracellular hyphae growth within leaf tissues led to the loss of host cell integrity and various levels of organelle disintegration. Severe symptoms of chloroplast damage included the degeneration of chloroplast envelope and grana, and the loss of electron denseness by stroma at the advanced stage of infection. Transcriptional profiling of infected leaves revealed that photosynthesis was the most negatively regulated biological process. However, in infected leaves, chlorophyll and carotenoid content did not decrease until 48 hpi, and several chlorophyll a fluorescence parameters, such as photosystem II quantum yield (Fv/Fm), non-photochemical quenching (NPQ), or plant vitality parameter (Rdf) decreased significantly at 24 and 48 hpi compared to control leaves. Our results indicate that the initial stages of interaction between B. oleracea and A. brassicicola are not uniform within an inoculation site and show a complexity of host responses and fungal attempts to overcome host cell defense mechanisms. The downregulation of photosynthesis at the early stage of this susceptible interaction suggests that it may be a part of a host defense strategy, or, alternatively, that chloroplasts are targets for the unknown virulence factor(s) of A. brassicicola. However, the observed decrease of photosynthetic efficiency at the later stages of infection is a result of the fungus-induced necrotic lesion expansion.

scholarly journals Genome Sequence of the Necrotrophic Plant Pathogen Alternaria brassicicola Abra43

2018 ◽  
Vol 6 (6) ◽  
pp. e01559-17 ◽  
Author(s):  
Elodie Belmas ◽  
Martial Briand ◽  
Anthony Kwasiborski ◽  
Justine Colou ◽  
Guillaume N’Guyen ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Plant Pathogen ◽  
Draft Genome ◽  
Black Spot ◽  
Fungal Diseases ◽  
Alternaria Brassicicola ◽  
Draft Genome Sequence ◽  
Dark Spot ◽  
Spot Disease ◽  
Black Spot Disease

ABSTRACT Alternaria brassicicola causes dark spot (or black spot) disease, which is one of the most common and destructive fungal diseases of Brassicaceae spp. worldwide. Here, we report the draft genome sequence of strain Abra43. The assembly comprises 29 scaffolds, with an N50 value of 2.1 Mb. The assembled genome was 31,036,461 bp in length, with a G+C content of 50.85%.

scholarly journals Survey and Pathogenicity of Black Spot Disease of Rose in Pakistan

2016 ◽  
Vol 03 (04) ◽  
Author(s):  
Yasin NA ◽  
Ahmed S ◽  
Khan WU ◽  
Ashraf Y
Keyword(s):  
Black Spot ◽  
Spot Disease ◽  
Black Spot Disease

scholarly journals Selection of Mutant Japanese Pears Resistant to Black Spot Disease by Acute Irradiation of Gamma-rays

1994 ◽  
Vol 62 (4) ◽  
pp. 701-706 ◽  
Author(s):  
Kenji Murata ◽  
Kenichi Kitagawa ◽  
Testuo Masuda ◽  
Kosuke Inoue ◽  
Kazuo Kotobuki ◽  
...  
Keyword(s):  
Gamma Rays ◽  
Black Spot ◽  
Spot Disease ◽  
Acute Irradiation ◽  
Selection Of ◽  
Black Spot Disease

Fungicidal activity of Thai medicinal plant extracts against Alternaria brassicicola causing black spot of Chinese kale

2018 ◽  
Vol 152 (1) ◽  
pp. 157-167 ◽  
Author(s):  
Tida Dethoup ◽  
Patcharavipha Songkumarn ◽  
Soraya Rueangrit ◽  
Supaporn Suesa-ard ◽  
Chutima Kaewkrajay
Keyword(s):  
Medicinal Plant ◽  
Plant Extracts ◽  
Fungicidal Activity ◽  
Black Spot ◽  
Alternaria Brassicicola ◽  
Chinese Kale ◽  
Medicinal Plant Extracts

Black spot disease ofLentinula edodescaused by theHyphozymasynanamorph ofEleutheromyces subulatus

Mycologia ◽  
1997 ◽  
Vol 89 (6) ◽  
pp. 867-875 ◽  
Author(s):  
Akihiko Tsuneda ◽  
Shigeyuki Murakami ◽  
Warwick M. Gill ◽  
Nitaro Maekawa
Keyword(s):  
Black Spot ◽  
Spot Disease ◽  
Black Spot Disease
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