The molecular detection of Fusarium oxysporum f. sp. dianthi races in protected carnation growing areas in western Turkey

2021 ◽  
Author(s):  
Ceren Cer ◽  
Seher Benlioglu ◽  
Yesim Egerci
Keyword(s):  
Fusarium Oxysporum ◽  
Molecular Detection ◽  
Western Turkey

scholarly journals Comparative analysis uncovers the limitations of current molecular detection methods for Fusarium oxysporum f. sp. cubense race 4 strains

PLoS ONE ◽  
2019 ◽  
Vol 14 (9) ◽  
pp. e0222727 ◽  
Author(s):  
Freddy Magdama ◽  
Lorena Monserrate-Maggi ◽  
Lizette Serrano ◽  
Daynet Sosa ◽  
David M. Geiser ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Fusarium Oxysporum ◽  
Molecular Detection ◽  
Detection Methods ◽  
Race 4

Molecular Detection of Antimicrobial Peptide Genes and Identification of Antifungal Compounds of Bacillus sp. against Fusarium oxysporum f. sp cubense Causing Wilt in Banana

2018 ◽  
Vol 28 (2) ◽  
pp. 1-8
Author(s):  
Rajesh Ponnusamy ◽  
Paranitharan Vaikuntavasan ◽  
Rajesh Subramanian ◽  
Kavino Mathiyazhagan ◽  
Muthulakshmi Palanisamy ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Fusarium Oxysporum ◽  
Molecular Detection ◽  
Antifungal Compounds ◽  
Bacillus Sp

Molecular detection and genotyping of Fusarium oxysporum f. sp. psidii isolates from different agro-ecological regions of India

2013 ◽  
Vol 51 (4) ◽  
pp. 405-412 ◽  
Author(s):  
Rupesh Kumar Mishra ◽  
Brajesh Kumar Pandey ◽  
Vijai Singh ◽  
Amita John Mathew ◽  
Neelam Pathak ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Molecular Detection ◽  
Ecological Regions

scholarly journals Pre-treatment and Suitable Reagent Enabled a Reliable and Consistent for Molecular Detection of Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc4)

2019 ◽  
Vol 26 (4) ◽  
pp. 196
Author(s):  
Listya Utami Karmawan ◽  
Fenny Martha Dwivany ◽  
Rizkita Rachmi Esyanti ◽  
I Nyoman Pugeg Aryantha
Keyword(s):  
Fusarium Oxysporum ◽  
Molecular Detection ◽  
Previous Method ◽  
Fungal Genome ◽  
Pcr Analysis ◽  
Banana Plant ◽  
Fungal Dna ◽  
Pre Treatment ◽  
Race 4 ◽  
Treatment Step

Fusarium wilt which is caused by the soil-borne fungus, Fusarium oxysporum f.sp. cubense (Foc), is one of important diseases in banana plant. Foc tropical race 4 (Foc4) is the most pathogenic race which could infect various banana cultivars including Cavendish cultivar which was previously considered as resistant cultivar. Molecular detection of Foc using PCR analysis is indispensable to determine the race of Foc. We demonstrate a faster DNA isolation procedure described in previous method by substituting sodium acetate precipitation with ammonium acetate precipitation without affecting the result. Based on our experience, some fungal genomes were troublesome to be amplified. We suggested pre-treatment step prior to amplification procedure by incubating fungal DNA in 65°C for 10 minutes for any samples of fungal genome, including stubborn samples, before mixing into PCR mix reagent. PCR reagents should be tested for stubborn samples since some of the reagents were unable to amplify the desired DNA fragment. Pre-treatment and the choice of robust PCR reagent should be taken into consideration for a reliable and consistent Foc4 molecular detection result.

scholarly journals Molecular Detection of Fusarium oxysporum f. sp. Cubense Race TR4 Infecting Banana Groups in Indo-Gangetic Plains of India

2020 ◽  
pp. 136-148
Author(s):  
Debashis Rana ◽  
Ankit Kumar Ghorai ◽  
Krishnendu Pramanik ◽  
Arpita Das ◽  
Joydeep Banerjee ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Molecular Detection ◽  
Disease Incidence ◽  
Intergenic Spacer ◽  
Asia Pacific ◽  
Economic Losses ◽  
Pathogenicity Test ◽  
Banana Production ◽  
Close Relationship ◽  
The Impact

Panama wilt or Fusarium wilt incited by Fusarium oxysporum f. sp. cubense (Foc) is a major threat for banana cultivation and impacting symbolic economic losses worldwide. Previously Foc race 1 and race 2 was detected in India which eventually spread monoculture of ‘Cavendish’ bananas resistant against these two races. Later on, another new race TR4 of Foc was detected to be incited in ‘Cavendish’ group of banana raised an alarming situation to the global banana industry. In the present study detailed survey was conducted in popular banana growing districts of West Bengal, India for visualizing the impact of disease incidence as well as collection of isolates from infected suckers and rhizomes of ‘Cavendish’ banana with an aim to develop a rapid detection technique for apprehending the presence of Foc TR4 race at early infection stages. Methods comprised of field survey followed by isolation of pathogen, test of pathogenicity and further molecular detection through Foc TR4 specific primers. Results revealed that the colonies formed on the potato dextrose agar (PDA) medium exhibited typical characteristic of Foc. Pathogenicity test conducted by considering healthy plantlet of ‘Grand Naine’ also detected characteristic symptom of Foc. Additionally, PCR assay using specific markers followed by sequencing of 28S-18S ribosomal RNA intergenic spacer spacer (IGS) confirmed the presence of this isolate in the infected samples. Phylogenetic evolution assays revealed a very close relationship of the Foc TR4 strains of India with Asia-Pacific isolates of Foc TR4. This study advocated the urgency of prevention of introduction of Foc TR4 into disease-free areas in the banana growing districts of India for maintaining sustainable banana production.

scholarly journals Pathogenic and molecular detection of Fusarium oxysporum f. sp. albedinis isolates from different areas in southwest Algeria

2018 ◽  
Vol 17 (8) ◽  
pp. 1629
Author(s):  
Larbi Benlarbi ◽  
Miloud Bellahcene ◽  
Lakhdar Mebarki ◽  
Corinne Vander Wauven ◽  
Bertrand Cornu ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Molecular Detection
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