Molecular characterization and infectivity analysis of a bipartite begomovirus associated with cotton leaf curl Multan betasatellite naturally infecting Rumex nepalensis in northern India

Soybean, Glycine max (L.) Merr., is a protein- and oil-rich crop cultivated in India and abroad. A yellow mosaic disease was observed on soybean with 80 to 90% disease incidence during August 2005 at fields of the National Botanical Research Institute, Lucknow, in northern India. Soybean plants were found to be infested with whiteflies (Bemisia tabaci) suggesting begomovirus etiology. The disease agent was transmitted experimentally by whiteflies, and symptoms developed after 23 days. Total DNA was isolated from 51 leaf samples collected from 42 symptomatic and 9 asymptomatic plants. Polymerase chain reaction was performed using begomovirus coat protein-specific primers 5′-ATGGCGAA GCGACCAG-3′ and 5′-TTAATTTGTGACCGAATCAT-3′ (AM180920/ AM180921). An amplicon of the expected size (~800 bp) was obtained in all 42 symptomatic leaves but not from any of the nine asymptomatic leaf samples. The amplicon was cloned, and the identical sequence of three clones was submitted to GenBank (Accession No. DQ343283). BLAST search of nucleotide sequences revealed 95% identity with Cotton leaf curl Kokhran virus (CLCKV) (GenBank Accession Nos. AJ002449, AJ002448, AJ496286, and AY456683) and 57% identity with Mungbean yellow mosaic India virus (MYMIV-Sb, GenBank Accession No. AY049772). Results indicated that the virus associated with yellow mosaic disease of soybean is an isolate of CLCKV rather than MYMIV-Sb (1) reported earlier on soybean from northern India. To our knowledge, this is the first report of soybean as a new host of Cotton leaf curl Kokhran virus. Reference: (1) K. S. Usharani et al. Curr. Sci. 86:845, 2004.

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Molecular characterization and phylogenetic analysis of tomato leaf curl Palampur virus, a bipartite begomovirus, associated with Cucumis sativus L. in Pakistan

3 Biotech ◽

10.1007/s13205-019-1727-3 ◽

2019 ◽

Vol 9 (6) ◽

Cited By ~ 2

Author(s):

Muhammad Shafiq ◽

Mukhtar Ahmad ◽

Ayesha Nisar ◽

Muhammad Tariq Manzoor ◽

Arslan Abid ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Molecular Characterization ◽

Cucumis Sativus ◽

Tomato Leaf ◽

Bipartite Begomovirus ◽

Cucumis Sativus L ◽

Tomato Leaf Curl ◽

Leaf Curl

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A monopartite begomovirus-associated DNA β satellite substitutes for the DNA B of a bipartite begomovirus to permit systemic infection

Journal of General Virology ◽

10.1099/vir.0.83049-0 ◽

2007 ◽

Vol 88 (10) ◽

pp. 2881-2889 ◽

Cited By ~ 99

Author(s):

Muhammad Saeed ◽

Yusuf Zafar ◽

John W. Randles ◽

M. Ali Rezaian

Keyword(s):

Systemic Infection ◽

Tomato Leaf ◽

Cauliflower Mosaic Virus ◽

35S Promoter ◽

Cotton Leaf ◽

Cell Periphery ◽

Bipartite Begomovirus ◽

Movement Function ◽

Tomato Leaf Curl ◽

Leaf Curl

DNA β is a circular single-stranded satellite DNA which co-infects with certain monopartite helper begomoviruses to cause economically important diseases, such as cotton leaf curl disease (CLCuD). DNA β encodes a single protein, βC1. Tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite begomovirus in which both DNA A and DNA B are required for systemic infection. Inoculation of tomato plants with ToLCNDV DNA A alone induced local but not systemic infection, whereas co-inoculation with DNA A and the DNA β associated with CLCuD resulted in systemic infection. DNA β containing a disrupted βC1 open reading frame (ORF) did not mobilize DNA A systemically. Co-inoculation of plants with DNA A and a construct of the βC1 ORF, under the control of the cauliflower mosaic virus 35S promoter, resulted in the systemic movement of DNA A. In inoculated tobacco and onion epidermal cells, βC1 fused to GFP was localized at the cell periphery in association with punctate bodies, around and within the cell nucleus and with the endoplasmic reticulum. It is concluded that heterologous βC1 protein can replace the movement function of the DNA B of a bipartite begomovirus. Evidence is also provided that tomato leaf curl virus-encoded C4 protein confers the same movement function to ToLCNDV DNA A. The intracellular distribution of βC1 is consistent with the hypothesis that it has a role in transporting the DNA A from the nuclear site of replication to the plasmodesmatal exit sites of the infected cell.

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Implication of the Whitefly Protein Vps Twenty Associated 1 (Vta1) in the Transmission of Cotton Leaf Curl Multan Virus

Microorganisms ◽

10.3390/microorganisms9020304 ◽

2021 ◽

Vol 9 (2) ◽

pp. 304

Author(s):

Yao Chi ◽

Li-Long Pan ◽

Shu-Sheng Liu ◽

Shahid Mansoor ◽

Xiao-Wei Wang

Keyword(s):

Coat Protein ◽

Molecular Mechanisms ◽

Cotton Leaf ◽

Yeast Two Hybrid ◽

Yeast Two Hybrid System ◽

Vacuolar Protein ◽

Leaf Curl Disease ◽

Two Hybrid ◽

Asia Ii 1 ◽

Leaf Curl

Cotton leaf curl Multan virus (CLCuMuV) is one of the major casual agents of cotton leaf curl disease. Previous studies show that two indigenous whitefly species of the Bemisia tabaci complex, Asia II 1 and Asia II 7, are able to transmit CLCuMuV, but the molecular mechanisms underlying the transmission are poorly known. In this study, we attempted to identify the whitefly proteins involved in CLCuMuV transmission. First, using a yeast two-hybrid system, we identified 54 candidate proteins of Asia II 1 that putatively can interact with the coat protein of CLCuMuV. Second, we examined interactions between the CLCuMuV coat protein and several whitefly proteins, including vacuolar protein sorting-associated protein (Vps) twenty associated 1 (Vta1). Third, using RNA interference, we found that Vta1 positively regulated CLCuMuV acquisition and transmission by the Asia II 1 whitefly. In addition, we showed that the interaction between the CLCuMuV coat protein and Vta1 from the whitefly Middle East-Asia Minor (MEAM1), a poor vector of CLCuMuV, was much weaker than that between Asia II 1 Vta1 and the CLCuMuV coat protein. Silencing of Vta1 in MEAM1 did not affect the quantity of CLCuMuV acquired by the whitefly. Taken together, our results suggest that Vta1 may play an important role in the transmission of CLCuMuV by the whitefly.

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