scholarly journals A Yellow Mosaic Disease of Soybean in Northern India is Caused by Cotton leaf curl Kokhran virus

Plant Disease ◽  
2006 ◽  
Vol 90 (7) ◽  
pp. 975-975 ◽  
Author(s):  
S. K. Raj ◽  
M. S. Khan ◽  
S. K. Snehi ◽  
S. Srivastava ◽  
H. B. Singh
Keyword(s):  
Disease Incidence ◽  
Mosaic Disease ◽  
Cotton Leaf ◽  
Yellow Mosaic Disease ◽  
New Host ◽  
Northern India ◽  
National Botanical Research Institute ◽  
Soybean Plants ◽  
Botanical Research ◽  
Leaf Curl

Soybean, Glycine max (L.) Merr., is a protein- and oil-rich crop cultivated in India and abroad. A yellow mosaic disease was observed on soybean with 80 to 90% disease incidence during August 2005 at fields of the National Botanical Research Institute, Lucknow, in northern India. Soybean plants were found to be infested with whiteflies (Bemisia tabaci) suggesting begomovirus etiology. The disease agent was transmitted experimentally by whiteflies, and symptoms developed after 23 days. Total DNA was isolated from 51 leaf samples collected from 42 symptomatic and 9 asymptomatic plants. Polymerase chain reaction was performed using begomovirus coat protein-specific primers 5′-ATGGCGAA GCGACCAG-3′ and 5′-TTAATTTGTGACCGAATCAT-3′ (AM180920/ AM180921). An amplicon of the expected size (~800 bp) was obtained in all 42 symptomatic leaves but not from any of the nine asymptomatic leaf samples. The amplicon was cloned, and the identical sequence of three clones was submitted to GenBank (Accession No. DQ343283). BLAST search of nucleotide sequences revealed 95% identity with Cotton leaf curl Kokhran virus (CLCKV) (GenBank Accession Nos. AJ002449, AJ002448, AJ496286, and AY456683) and 57% identity with Mungbean yellow mosaic India virus (MYMIV-Sb, GenBank Accession No. AY049772). Results indicated that the virus associated with yellow mosaic disease of soybean is an isolate of CLCKV rather than MYMIV-Sb (1) reported earlier on soybean from northern India. To our knowledge, this is the first report of soybean as a new host of Cotton leaf curl Kokhran virus. Reference: (1) K. S. Usharani et al. Curr. Sci. 86:845, 2004.

Survey for Yellow Mosaic Disease Occurring on Blackgram [Vigna mungo (L.) Hepper] and Weed Hosts in Four Major Blackgram Growing Districts of Andhra Pradesh and Characterization of Associated Viruses using Coat Protein Gene

2021 ◽  
Author(s):  
Vallabhaneni Tilak Chowdary ◽  
V. Manoj Kumar ◽  
P. Kishore Varma ◽  
B. Sreekanth ◽  
V. Srinivasa Rao
Keyword(s):  
Coat Protein ◽  
Andhra Pradesh ◽  
Disease Incidence ◽  
Pcr Amplification ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Ageratum Conyzoides ◽  
Yellow Mosaic Disease ◽  
Weed Plants ◽  
Weed Hosts

Background: Yellow mosaic disease (YMD) caused by Yellow mosaic virus is one of the major constraints in the pulse production in Andhra Pradesh (A.P.) due to fast evolution of strains, like Mungbean yellow mosaic India virus (MYMIV). Keeping this in view, a survey was undertaken in the major blackgram growing districts of A.P. to know the YMD incidence in blackgram and weed hosts and were characterized based on genetic features by comparing with other YMV isolates from different hosts and locations across the world. Methods: Roving survey was conducted during rabi 2019-20 in major blackgram growing districts of A.P. viz., Krishna, Guntur, West Godavari and Prakasam districts for YMD incidence. Blackgram plants showing characteristic symptoms were collected as representative samples from each mandal along with the suspected weed plants and were subjected to amplification using coat protein (CP) specific primers followed by molecular characterization. Phylogenetic tree for coat protein (CP) gene was constructed using aligned sequences with 1000 bootstrap replicates following neighbor-joining phylogeny. Result: Out of the four districts surveyed, the highest disease incidence was recorded at Machavaram village of Prakasam district (43.22%), whereas least disease incidence was recorded at Chinaganjam village of Praksam district (2.4%). Six weeds viz., Ageratum conyzoides, Amaranthus viridis, Parthenium hysterophporus, Vigna trilobata, Abelmoscus moschatus, Desmodium laxiflorum have showed positive result in PCR amplification with MYMIV specific coat protein primers. Four isolates from blackgram samples and two from weed plants shared 94.85 to 99.58% nucleotide identity among themselves.

Appearance of Cotton leaf curl disease begomovirus avirulent strains associated with a lower disease incidence in Delhi

2020 ◽  
Vol 28 (1) ◽  
pp. 67
Author(s):  
Manisha Duhan ◽  
Subhankar Gupta ◽  
U.K. Bhattacharyya ◽  
S. Palchoudhury ◽  
K.K. Biswas
Keyword(s):  
Disease Incidence ◽  
Cotton Leaf ◽  
Cotton Leaf Curl Disease ◽  
Leaf Curl Disease ◽  
Leaf Curl

scholarly journals Genetic variability of Cotton leaf curl betasatellite in Northern India

2014 ◽  
Vol 21 (6) ◽  
pp. 626-631 ◽  
Author(s):  
Sayed Sartaj Sohrab ◽  
Esam I. Azhar ◽  
Mohammad A. Kamal ◽  
P.S. Bhattacharya ◽  
D. Rana
Keyword(s):  
Genetic Variability ◽  
Cotton Leaf ◽  
Northern India ◽  
Leaf Curl

scholarly journals Management of Yellow Mosaic Disease of Mungbean Against Mungbean Yellow Mosaic Virus (MYMV) in Bangladesh

2018 ◽  
Vol 1 (2) ◽  
pp. p100
Author(s):  
Md. S. Islam ◽  
Md. B. Hossain ◽  
Saleh A. Shahriar ◽  
Fatema Begum ◽  
Md. N. H. Sani
Keyword(s):  
Mosaic Virus ◽  
Disease Severity ◽  
Agricultural Research ◽  
Disease Incidence ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Economic Damage ◽  
Yellow Mosaic Disease ◽  
Yield Attributes ◽  
Mungbean Yellow Mosaic Virus

The prime aim of the study was to manage of Yellow mosaic disease of mungbean against Mungbean yellow mosaic virus (MYMV) by using one newly release botanical nutrient and through three selected insecticides. BARI (Bangladesh Agricultural Research Institute) released variety BARI mung-5, three insecticides (Imidacloprid, Acmix and Sobicron) and one botanical nutrient PPN (Peak performance nutrients) were used in the experiment. The plants were grown for pulse production and natural inoculums were relied upon for the infection of MYMV. Growth parameters, yield attributes and physiological features were significantly influenced by the application of selected insecticides and PPN combinations. Disease incidence and disease severity of MYMV were significantly varied among the treatments. Application of Imidacloprid with PPN combination gave the lowest disease incidence (3.13, 5.24 and 6.24% per plot and 14.33, 15.49 and 21.87% per plant) at 30, 40 and 50 DAS, respectively while the highest disease incidence (7.77, 13.70 and 19.24% per plot and 39.33, 48.20 and 56.63% per plant) were found in control at 30, 40 and 50 DAS, respectively. Application of Imidacloprid with PPN also gave the lowest disease severity (5.00, 6.00 and 13.33% at 30, 40 and 50 DAS, respectively while the highest disease severity (27.33, 35.00 and 45.00%) at 30, 40 and 50 DAS, respectively were measured in control treatment when no insecticides and PPN was used. If the disease is established once in the field then it is difficult to manage. As the disease is transmitted by vector (whitefly), the growers are suggested to control the vector populations before reaching economic damage and severe disease infection.

scholarly journals The Association of Begomovirus with Bitter Melon in India

Plant Disease ◽  
2002 ◽  
Vol 86 (3) ◽  
pp. 328-328 ◽  
Author(s):  
J. A. Khan ◽  
M. K. Siddiqui ◽  
B. P. Singh
Keyword(s):  
Disease Incidence ◽  
Severe Disease ◽  
Cotton Leaf ◽  
Bitter Melon ◽  
Medicinal Value ◽  
Cotton Leaf Curl Virus ◽  
Pcr Products ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

Bitter melon, Momordica charantia (Cucurbitaceae), is a vegetable of nutritive and medicinal value that is cultivated throughout India and other tropical countries. In September 2001, a severe disease of bitter melon with virus-like symptoms was observed at Lucknow, India. Symptoms consisted of upward curling, shortening, and distortion of leaves. Diseased melon fruits were stunted and deformed. Disease incidence was as high as 100%. Whitefly (Bemicia tabaci) can transmit the associated virus from diseased bitter melon to Nicotiana tabacum cv. White burley. The development of leaf curl symptoms in N. tabacum indicated the pathogen could be a begomovirus. Total nucleic acids were extracted from diseased bitter melon leaves, and polymerase chain reaction (PCR) tests were performed. Three pairs of primers, AV494 and AC1048 (1), CL-CR/F2 and CL-CR/R2, CL/11F and CL10/R (2), specific to DNA-A of begomoviruses were used in PCR. Virus-specific DNA-A fragments of expected sizes were identified (≈0.5, 0.7 and 1.2 kb, respectively). The presence of a begomovirus in all PCR-amplified DNA fragments was confirmed by Southern hybridization. Cloned DNA-A fragments of Tomato leaf curl virus and Cotton leaf curl virus (both begomoviruses) cross-hybridized with the PCR products gave strong signals under high stringency conditions. These data suggest that a begomovirus is associated with this bitter melon disease. Watermelon mosaic 1 virus is the only virus previously reported to naturally infect bitter melon; however, this virus has not been identified in India. Bitter melon is also an experimental host of Ribgrass mosaic virus (genus Tobamovirus) and Trichosanthes mottle virus (genus Potyvirus). To our knowledge, this is the first report of the occurrence of begomovirus infecting bitter melon. References: (1) S. D. Wyatt and J. K. Brown. Phytopathology 86:1288, 1996. (2). X. Zhou et al. J. Gen. Virol. 79:915, 1998.

scholarly journals First Report of Association of Tomato leaf curl virus-New Delhi with Yellow Mosaic Disease of Luffa cylindrica in India

Plant Disease ◽  
2003 ◽  
Vol 87 (9) ◽  
pp. 1148-1148 ◽  
Author(s):  
S. S. Sohrab ◽  
B. Mandal ◽  
R. P. Pant ◽  
A. Varma
Keyword(s):  
Mosaic Disease ◽  
Tomato Leaf ◽  
New Delhi ◽  
Luffa Cylindrica ◽  
Yellow Mosaic Disease ◽  
Tomato Leaf Curl Virus ◽  
Sponge Gourd ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

Sponge gourd (Luffa cylindrica), an important cucurbitaceous vegetable in India, is affected by a disease (2) causing yellow spots on newly emerged leaves, mosaic, mild leaf curling and distortion, small leaves, and misshapen fruits. Nearly 100% of sponge gourd plants were symptomatic in Delhi. Geminivirus-like particles were observed with electron microscopy of uranyl acetate-stained leaf-dip preparations of the diseased plants collected from experimental fields at the Indian Agricultural Research Institute in New Delhi during May and June of 2002. The virus was transmitted by the whitefly (Bemisia tabaci) to sponge and ridge gourd (L. acutangula) after an acquisition and inoculation access period of 24 h each. Whitefly-inoculated plants produced typical yellow mosaic symptoms and contained geminate particles. Nucleic acid extracted from the field-infected and experimentally infected plants hybridized with 32P-labeled probe to DNA-A of Indian cassava mosaic virus, suggesting association of a begomovirus. The viral DNA, isolated by the alkali denaturation method (1) from the experimentally infected sponge gourd plants, was cloned in pBS SK+ at the EcoRI site. A clone with an insert of 2,658 bp was sequenced (GenBank Accession Nos. AJ557219, AJ555488, and AY309957) which shared 89.6 to 95.1% identity with the DNA-A of different strains of Tomato leaf curl virus-New Delhi (ToLCV-NDe). The highest sequence identity (95.1%) was with the severe strain of ToLCV-NDe (GenBank Accession No. U15015). The data suggest that the begomovirus associated with the yellow mosaic disease of L. cylindrica in India is a putative strain of ToLCV-NDe. Reference: (1) K. M. Srivastava et al. J. Virol. Methods 51:297, 1995. (2) A. Varma and B. K. Giri. Virus diseases. Pages 225–245 in: Cucurbits. N. M. Nayar and T. A More, eds. Oxford and IBH Publishing House Private Ltd., New Delhi, India, 1998.

Occurrence of Begomovirus Associated with Yellow Vein Mosaic Disease of Kenaf (Hibiscus cannabinus) in Northern India

2007 ◽  
Vol 8 (1) ◽  
pp. 21 ◽  
Author(s):  
Raju Ghosh ◽  
Sujay Paul ◽  
Anirban Roy ◽  
Javid Iqbal Mir ◽  
S. K. Ghosh ◽  
...  
Keyword(s):  
Southern Hybridization ◽  
Disease Incidence ◽  
Pcr Amplification ◽  
Uttar Pradesh ◽  
Mosaic Disease ◽  
Hibiscus Cannabinus ◽  
Yellow Vein ◽  
Specific Probe ◽  
Northern India ◽  
Yellow Vein Mosaic Disease

The occurrence, distribution, and disease incidence of yellow vein mosaic (YVM) disease of kenaf (Hibiscus cannabinus) in northern India are reported. The disease was found in both commercial and experimental farms in the Bahraich district, Uttar Pradesh, India and caused significant reduction in plant height and crop yield. Southern hybridization with begomovirus-specific probe and PCR amplification with DNA β and coat protein primers confirmed the association of begomovirus with the disease. Accepted for publication 25 January 2007. Published 8 May 2007.

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