Establishment of adventitious root culture from leaf explants of Plumbago zeylanica: an endangered medicinal plant

2021 ◽  
Author(s):  
Kajal Katoch ◽  
Suphla Gupta ◽  
Romaan Nazir ◽  
Vijay Kumar ◽  
Rupa Sanyal ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Adventitious Root ◽  
Leaf Explants ◽  
Root Culture ◽  
Plumbago Zeylanica ◽  
Endangered Medicinal Plant ◽  
Adventitious Root Culture

scholarly journals Establishment of In Vitro Adventitious Root Cultures and Analysis of Andrographolide in Andrographis Paniculata

2013 ◽  
Vol 8 (8) ◽  
pp. 1934578X1300800
Author(s):  
Shiv Narayan Sharma ◽  
Zenu Jha ◽  
Rakesh Kumar Sinha
Keyword(s):  
Adventitious Root ◽  
Adventitious Roots ◽  
Leaf Explants ◽  
Root Culture ◽  
Andrographis Paniculata ◽  
Ms Medium ◽  
Plant Parts ◽  
Bioactive Component ◽  
Adventitious Root Culture

Andrographolide is the principal bioactive component of the medicinal plant Andrographis paniculata, to which various diverse pharmacological properties are attributed. Traditionally, andrographolide was extracted from the leaves, stems and other parts of the plant. Leaves have the highest andrographolide content (2–3%) in comparison with the other plant parts. Adventitious root culture of leaf explants of A. paniculata was studied using different strength MS medium supplemented by different concentrations of auxins and a combination of NAA + kinetin for growth and andrographolide production. Among the different auxin treatments in adventitious root culture, only NAA was able to induce adventitious roots. Adventitious roots grown in modified strength MS medium showed the highest root growth (26.7±1.52), as well as the highest amount of andrographolide (133.3±1.5 mg/g DW) as compared with roots grown in half-and full-strength MS medium. Growth kinetics showed maximum biomass production after five weeks of culture in different strength MS liquid medium. The produced andrographolide content was 3.5- 5.5 folds higher than that of the natural plant, depending on the medium strength.

scholarly journals Establishment of In vitro Adventitious Root Cultures and Analysis of Flavonoids in Rumex crispus

2015 ◽  
Vol 25 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Majid Mahdieh ◽  
Mitra Noori ◽  
Simin Hoseinkhani
Keyword(s):  
Adventitious Root ◽  
Adventitious Roots ◽  
Culture Media ◽  
Leaf Explants ◽  
Root Culture ◽  
Root Extract ◽  
Rumex Crispus ◽  
Adventitious Root Culture ◽  
Flavonoid Composition

Adventitious root culture of leaf explants of R. crispus was established using different MS supplemented with different concentrations of auxins and a combination of NAA and Kn for growth and flavonoids production. Among the different auxins, NAA was more effective than IAA to induce adventitious roots. Adventitious roots grown on MS containing 5 ?M NAA and 0.5 ?M Kn showed the highest root growth, as well as the highest amount of total flavonoids (= 6) as compared with roots grown in other media. Chromatographic purification of the root extract showed that flavonoid composition also was influenced by hormone combinations in the culture media. The addition of Kn to the medium reduced or suppressed myricetin (M) and naringenin (N) production. Quercetin (Q) was not found in media containing Kn alone similar to the control medium. Isorhamnetin (I), kaempferol (K) and rutin (R) were produced in the roots on media supplemented with all hormone combinations, but were absent in 0.1 ?M Kn supplemented media similar to the control roots.Plant Tissue Cult. & Biotech. 25(1): 63-70, 2015 (June)

Adventitious root culture of Pfaffia glomerata (Spreng.) Pedersen in a roller bottle system: An alternative source of β-ecdysone

2021 ◽  
Vol 43 ◽  
pp. 1-7
Author(s):  
Thaila Fernanda Oliveira da Silva ◽  
Cristina Sayuri Yamaguchi ◽  
Susana Tavares Cotrim Ribeiro ◽  
Alexandre da Silva Avincola ◽  
Eduardo Jorge Pilau ◽  
...  
Keyword(s):  
Adventitious Root ◽  
Root Culture ◽  
Roller Bottle ◽  
Alternative Source ◽  
Pfaffia Glomerata ◽  
Adventitious Root Culture

Factors influencing podophyllotoxin production in adventitious root culture of Podophyllum hexandrum Royle

2014 ◽  
Vol 36 (4) ◽  
pp. 1009-1021 ◽  
Author(s):  
Manoharan Rajesh ◽  
Ganeshan Sivanandhan ◽  
Muthukrishnan Arun ◽  
Venkatachalam Vasudevan ◽  
Jeevaraj Theboral ◽  
...  
Keyword(s):  
Adventitious Root ◽  
Root Culture ◽  
Podophyllum Hexandrum ◽  
Factors Influencing ◽  
Adventitious Root Culture

Production of secoiridoids by adventitious root culture of Swertia japonica

2015 ◽  
Vol 51 (5) ◽  
pp. 524-529 ◽  
Author(s):  
Hiroko Kawakami ◽  
Kojiro Hara ◽  
Masashi Komine ◽  
Yoshikazu Yamamoto
Keyword(s):  
Adventitious Root ◽  
Root Culture ◽  
Adventitious Root Culture ◽  
Swertia Japonica
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