Mesta yellow vein mosaic virus: application of loop-mediated isothermal amplification method to study efficiency of acquisition, retention and transmission by Bemisia tabaci (Hemiptera: Aleyrodidae) in Kenaf

2020 ◽  
Author(s):  
P. N. Meena ◽  
B. S. Gotyal ◽  
S. Satpathy
Keyword(s):  
Mosaic Virus ◽  
Bemisia Tabaci ◽  
Isothermal Amplification ◽  
Yellow Vein ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Method ◽  
Yellow Vein Mosaic Virus

Detection of Mesta yellow vein mosaic virus (MeYVMV) in field samples by a loop-mediated isothermal amplification reaction

2019 ◽  
Vol 263 ◽  
pp. 81-87 ◽  
Author(s):  
Prabhu Narayan Meena ◽  
Lalit Laxman Kharbikar ◽  
Rajeev Singh Rana ◽  
Subrata Satpathy ◽  
Arti Shanware ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Isothermal Amplification ◽  
Yellow Vein ◽  
Loop Mediated Isothermal Amplification ◽  
Yellow Vein Mosaic Virus ◽  
Field Samples ◽  
Amplification Reaction

scholarly journals Comparative transmission of Bhendi yellow vein mosaic virus by two cryptic species of the whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)

3 Biotech ◽  
2017 ◽  
Vol 7 (5) ◽  
Author(s):  
V. Venkataravanappa ◽  
M. H. Kodandaram ◽  
C. N. Lakshminarayana Reddy ◽  
K. S. Shankarappa ◽  
M. Krishna Reddy
Keyword(s):  
Mosaic Virus ◽  
Cryptic Species ◽  
Bemisia Tabaci ◽  
Yellow Vein ◽  
Yellow Vein Mosaic Virus

scholarly journals P02.06 A Novel Loop-Mediated Isothermal Amplification Method for Robust Detection of EGFR Mutations

2021 ◽  
Vol 16 (3) ◽  
pp. S248-S249
Author(s):  
Y. Saito ◽  
A. Matsui ◽  
S. Michiyuki ◽  
Y. Yamauchi ◽  
N. Takahashi ◽  
...  
Keyword(s):  
Isothermal Amplification ◽  
Egfr Mutations ◽  
Robust Detection ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Method

scholarly journals Sensitive and Rapid Detection of Edwardsiellosis in Fish by a Loop-Mediated Isothermal Amplification Method

2004 ◽  
Vol 70 (1) ◽  
pp. 621-624 ◽  
Author(s):  
Ram Savan ◽  
Arisa Igarashi ◽  
Satoru Matsuoka ◽  
Masahiro Sakai
Keyword(s):  
Rapid Detection ◽  
Japanese Flounder ◽  
Isothermal Amplification ◽  
Edwardsiella Tarda ◽  
Fish Disease ◽  
Fish Pathogen ◽  
Loop Mediated Isothermal Amplification ◽  
Diagnostic Protocol ◽  
Amplification Method ◽  
Hemolysin Gene

ABSTRACT Here we report a rapid and sensitive method (using loop-mediated isothermal amplification [LAMP]) for the diagnosis of edwardsiellosis, a fish disease caused by Edwardsiella tarda, in Japanese flounder. A set of four primers was designed, and conditions for the detection were optimized for the detection of E. tarda in 45 min at 65°C. No amplification of the target hemolysin gene was detected in other related bacteria. When the LAMP primers were used, detection of edwardsiellosis in infected Japanese flounder kidney, and spleen and seawater cultures was possible. We have developed a rapid and sensitive diagnostic protocol for edwardsiellosis detection in fish. This is the first report of the application of LAMP for the diagnosis of a fish pathogen.

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