Establishment and Characterization of a Stromal Cell Line Derived From a Patient With Thoracic Endometriosis

We have used a biological assay system we developed to biochemically purify a previously uncharacterized murine lymphopoietic growth factor designated lymphopoietin 1 (LP-1). This factor is capable of stimulating the proliferation and extended maintenance of precursor cells of the B lineage. A stromal cell line producing LP-1 was established after transfection of primary stromal cultures with a plasmid encoding the transforming genes of SV40. This factor was purified to a single 25-kD species from the culture supernatant of an adherent stromal cell line. This material acts on immature lymphocytes, it binds to specific receptors on cells, and is distinct from previously described hematopoietic factors. LP-1 has been purified some 10(7)-fold with an overall recovery of 35%. The purified protein exhibits a specific activity of approximately 4 X 10(6) U/micrograms of protein and is active at a half-maximal concentration of 10(-13) M.

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Phenotypic and functional characterization of a marrow-derived stromal cell line, M210B4 and its comparison with primary marrow stromal cells

Biomedical Research Journal ◽

10.4103/2349-3666.240617 ◽

2015 ◽

Vol 2 (1) ◽

pp. 120 ◽

Cited By ~ 2

Author(s):

Vaijayanti Kale ◽

Shweta Singh ◽

Suprita Ghode ◽

MoirangthemRanjita Devi ◽

Lalita Limaye

Keyword(s):

Cell Line ◽

Stromal Cells ◽

Stromal Cell ◽

Functional Characterization ◽

Marrow Stromal Cells ◽

Stromal Cell Line

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Establishment and characterization of a pronephric stromal cell line (TPS) from rainbow trout,Oncorhynchus mykissW.

Fish & Shellfish Immunology ◽

10.1006/fsim.1995.0042 ◽

1995 ◽

Vol 5 (6) ◽

pp. 441-457 ◽

Cited By ~ 12

Author(s):

M.L. Diago ◽

M.P. López-Fierro ◽

B. Razquin ◽

A. Villena

Keyword(s):

Rainbow Trout ◽

Cell Line ◽

Stromal Cell ◽

Stromal Cell Line

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Molecular Cloning and Biological Characterization of a Novel Murine Lymphoid Growth Factor

Journal of Experimental Medicine ◽

10.1084/jem.192.5.671 ◽

2000 ◽

Vol 192 (5) ◽

pp. 671-680 ◽

Cited By ~ 160

Author(s):

John E. Sims ◽

Douglas E. Williams ◽

Philip J. Morrissey ◽

Kirsten Garka ◽

Diane Foxworthe ◽

...

Keyword(s):

B Cell ◽

Cell Line ◽

Stromal Cell ◽

Biological Characterization ◽

Gene Encoding ◽

Interleukin 7 ◽

Stromal Cell Line ◽

Thymic Stromal Cell

Using a bioassay consisting of the proliferation of a murine B cell line, a cDNA of a gene whose product supports the growth of that cell line was isolated from a thymic stromal cell line. This factor, termed thymic stromal lymphopoietin (TSLP), is a protein of 140 amino acids. The gene encoding TSLP was mapped to murine chromosome 18. Purified recombinant TSLP supported the growth of pre-B cell colonies in vitro, but had no myelopoietic activity. TSLP had comitogenic activity for fetal thymocytes, but was not as potent as interleukin 7 in lobe submersion cultures. Injection of TSLP into neonatal mice induced the expansion of B220+BP-1+ pre-B cells.

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