Cortical Reaction | ScienceGate

The aim of this study was to investigate the process of the cortical reaction in eggs of pikeperch, Sander lucioperca (L.), as well as the application of microscopic assessment of this process in egg quality evaluation. The analysis was carried out with eggs obtained from 10 females by artificial reproduction, in which hormonal stimulation with hCG was applied. Subsequently, each sample of eggs (separately from each female fish) was analysed. The analysis included observation of the cortical reaction and the process of egg swelling, and determination of the effect of temperature (12, 14 and 16°C) and the presence of spermatozoa on the cortical reaction. The results indicate that the cortical reaction in pikeperch eggs is quite violent, resulting in visible deformation of eggs between 3 and 5 min after activation. No effect of temperature or the presence of spermatozoa on the cortical reaction was observed. A strong correlation was recorded for the percentage of egg deformations observed and embryo survival rate. The described method of determination of pikeperch egg quality (based on egg deformation rate between 3 and 5 min after activation) may be highly useful, both in scientific research (where high-quality eggs are required) and in hatchery practice.

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The cortical reaction and development of activation competence in mammalian oocytes

Human Reproduction Update ◽

10.1093/humupd/2.1.29 ◽

1996 ◽

Vol 2 (1) ◽

pp. 29-42 ◽

Cited By ~ 82

Author(s):

T Ducibella

Keyword(s):

Cortical Reaction

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Early development of the horseshoe crab, Limulus polyphemus, L.: 1) ultrastructure of the cortical reaction and amino acid incorporation during egg activation, and 2) ultrastructure and protein synthesis of the extra-embryonic shell

10.31274/rtd-180816-4650 ◽

1981 ◽

Author(s):

Gary Anthony Bannon

Keyword(s):

Protein Synthesis ◽

Amino Acid ◽

Early Development ◽

Horseshoe Crab ◽

Limulus Polyphemus ◽

Egg Activation ◽

Amino Acid Incorporation ◽

Cortical Reaction ◽

Acid Incorporation ◽

Embryonic Shell

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OOCYTE DIFFERENTIATION IN THE SEA URCHIN, ARBACIA PUNCTULATA, WITH PARTICULAR REFERENCE TO THE ORIGIN OF CORTICAL GRANULES AND THEIR PARTICIPATION IN THE CORTICAL REACTION

The Journal of Cell Biology ◽

10.1083/jcb.37.2.514 ◽

1968 ◽

Vol 37 (2) ◽

pp. 514-539 ◽

Cited By ~ 198

Author(s):

Everett Anderson

Keyword(s):

Sea Urchin ◽

Golgi Complex ◽

Morphological Evidence ◽

Cortical Granule ◽

Perivitelline Space ◽

Unit Membrane ◽

Cortical Granules ◽

Arbacia Punctulata ◽

Cortical Reaction ◽

Oocyte Differentiation

This paper presents morphological evidence on the origin of cortical granules in the oocytes of Arbacia punctulata and other echinoderms. During oocyte differentiation, those Golgi complexes associated with the production of cortical granules are composed of numerous saccules with companion vesicles. Each element of the Golgi complex contains a rather dense homogeneous substance. The vesicular component of the Golgi complex is thought to be derived from the saccular member by a pinching-off process. The pinched-off vesicles are viewed as containers of the precursor(s) of the cortical granules. In time, they coalesce and form a mature cortical granule whose content is bounded by a unit membrane. Thus, it is asserted that the Golgi complex is involved in both the synthesis and concentration of precursors utilized in the construction of the cortical granule. Immediately after the egg is activated by the sperm the primary envelope becomes detached from the oolemma, thereby forming what we have called the activation calyx (see Discussion). Subsequent to the elaboration of the activation calyx, the contents of cortical granules are released (cortical reaction) into the perivitelline space. The discharge of the constituents of a cortical granule is accomplished by the union of its encompassing unit membrane, in several places, with the oolemma.

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