Healing of Endothelium and Descemet's Membrane of Rabbit Cornea*

1958 ◽  
Vol 46 (1) ◽  
pp. 62-67 ◽  
Author(s):  
P.L. Morton ◽  
H.L. Ormsby ◽  
P.K. Basu
Keyword(s):  
Rabbit Cornea ◽  
Descemet's Membrane ◽  
Descemet’S Membrane

STUDIES ON PROTEIN METABOLISM OF THE CORNEA: 1. THE DISTRIBUTION OF NITROGENOUS SOLIDS IN RABBIT CORNEAS

1963 ◽  
Vol 41 (4) ◽  
pp. 1005-1011 ◽  
Author(s):  
R. C. French ◽  
Z. Duma
Keyword(s):  
Nitrogen Content ◽  
Protein Metabolism ◽  
Physiological Saline ◽  
Dry Weight ◽  
Weight Basis ◽  
Rabbit Cornea ◽  
Corneal Layer ◽  
Descemet's Membrane ◽  
Descemet’S Membrane ◽  
Cornea Endothelium

The normal rabbit cornea was found to contain an average of 22.7% solids (77.3% moisture), nitrogen comprising 14.7% of the dry weight. On a dry weight basis the cornea was composed of 10% as epithelium, 87% as stroma, and 3% as endothelium plus Descemet's membrane. The solid and nitrogen content of the stroma was nearly identical with that of the whole cornea. Endothelium plus Descemet's membrane and epithelium layers were relatively low in nitrogen content (10 and 12% of the dry weight, respectively) while the epithelium was relatively high in solids (30%). The partition of nitrogen, as protein and nonprotein, soluble or insoluble in physiological saline, was estimated for each corneal layer.

STUDIES ON PROTEIN METABOLISM OF THE CORNEA: 1. THE DISTRIBUTION OF NITROGENOUS SOLIDS IN RABBIT CORNEAS

1963 ◽  
Vol 41 (1) ◽  
pp. 1005-1011
Author(s):  
R. C. French ◽  
Z. Duma
Keyword(s):  
Nitrogen Content ◽  
Protein Metabolism ◽  
Physiological Saline ◽  
Dry Weight ◽  
Weight Basis ◽  
Rabbit Cornea ◽  
Corneal Layer ◽  
Descemet's Membrane ◽  
Descemet’S Membrane ◽  
Cornea Endothelium

The normal rabbit cornea was found to contain an average of 22.7% solids (77.3% moisture), nitrogen comprising 14.7% of the dry weight. On a dry weight basis the cornea was composed of 10% as epithelium, 87% as stroma, and 3% as endothelium plus Descemet's membrane. The solid and nitrogen content of the stroma was nearly identical with that of the whole cornea. Endothelium plus Descemet's membrane and epithelium layers were relatively low in nitrogen content (10 and 12% of the dry weight, respectively) while the epithelium was relatively high in solids (30%). The partition of nitrogen, as protein and nonprotein, soluble or insoluble in physiological saline, was estimated for each corneal layer.

Cleavage plane after liquid‐bubble preparation of Descemet’s membrane

2020 ◽  
Author(s):  
Annekatrin Rickmann ◽  
Silke Wahl ◽  
Alisa Katsen‐Globa ◽  
André Schulz ◽  
Norbert Pütz ◽  
...  
Keyword(s):  
Cleavage Plane ◽  
Descemet's Membrane ◽  
Liquid Bubble ◽  
Descemet’S Membrane

scholarly journals Corneal pachymetry by AS-OCT after Descemet’s membrane endothelial keratoplasty

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Friso G. Heslinga ◽  
Ruben T. Lucassen ◽  
Myrthe A. van den Berg ◽  
Luuk van der Hoek ◽  
Josien P. W. Pluim ◽  
...  
Keyword(s):  
Deep Learning ◽  
Endothelial Function ◽  
Corneal Thickness ◽  
Anterior Segment ◽  
Anatomical Landmarks ◽  
Endothelial Keratoplasty ◽  
Descemet’S Membrane Endothelial Keratoplasty ◽  
Descemet's Membrane ◽  
Descemet’S Membrane ◽  
Thickness Measurements

AbstractCorneal thickness (pachymetry) maps can be used to monitor restoration of corneal endothelial function, for example after Descemet’s membrane endothelial keratoplasty (DMEK). Automated delineation of the corneal interfaces in anterior segment optical coherence tomography (AS-OCT) can be challenging for corneas that are irregularly shaped due to pathology, or as a consequence of surgery, leading to incorrect thickness measurements. In this research, deep learning is used to automatically delineate the corneal interfaces and measure corneal thickness with high accuracy in post-DMEK AS-OCT B-scans. Three different deep learning strategies were developed based on 960 B-scans from 50 patients. On an independent test set of 320 B-scans, corneal thickness could be measured with an error of 13.98 to 15.50 μm for the central 9 mm range, which is less than 3% of the average corneal thickness. The accurate thickness measurements were used to construct detailed pachymetry maps. Moreover, follow-up scans could be registered based on anatomical landmarks to obtain differential pachymetry maps. These maps may enable a more comprehensive understanding of the restoration of the endothelial function after DMEK, where thickness often varies throughout different regions of the cornea, and subsequently contribute to a standardized postoperative regime.

Descemet's membrane detachment in horses; case series and literature review

2014 ◽  
Vol 18 (5) ◽  
pp. 357-363 ◽  
Author(s):  
Màrian Matas Riera ◽  
David Donaldson ◽  
Simon Lawrence Priestnall
Keyword(s):  
Literature Review ◽  
Case Series ◽  
Descemet’S Membrane Detachment ◽  
Descemet's Membrane ◽  
Descemet’S Membrane

Histological Studies on Ageing Changes in the Cornea of Indian Buffaloes (Bubalus bubalis)

2021 ◽  
Author(s):  
A. Prasanth Babu ◽  
P. Jagapathi Ramayya ◽  
Y. Nagamalleswari ◽  
Makkena Sreenu ◽  
K. Lakshmi Kavitha
Keyword(s):  
Corneal Epithelium ◽  
Corneal Stroma ◽  
Economic Loss ◽  
Progressive Increase ◽  
Group Iii ◽  
Age Related ◽  
Group I ◽  
Descemet's Membrane ◽  
Descemet’S Membrane ◽  
The Mean

Background: Affections of eye commonly encountered in all the species of animals. If these not treated in time, the vision may be hampered, this may impair the physical ability, utility and productivity of animals leading to economic loss to the animal owners. Corneal dystrophies like basement membrane dystrophy, stromal dystrophy, endothelial dystrophy, corneal erosions and corneal ulcerations etc., seen in the aged buffaloes. The current study was carried out to establish basic data and to provide authentic information to the clinicians on ageing changes in eyes of buffaloes.Methods: The study on age related changes in the cornea was conducted on 63 eye balls of locally available buffaloes of different breeds in and around Proddatur. These buffaloes were categorized into 3 groups based on their age i.e., group I (1-5 yrs), group II (6-10 yrs) and group III (11 yrs and above) buffaloes. The eyeballs were isolated and fixed with Davidson’s fluid. The paraffin sections of cornea were subjected for routine histological study.Result: The mean total thickness (µm) of cornea was increased from group I to III animals and it was 747.22±13.57, 853.33±11.05 and 897.22±8.06. Hence, The mean thickness (µm) of all five layers of cornea increased with advancement of age. Superficial corneal epithelial layer was made up of non keratinized stratified squamous epithelium with 6 to 7 layers at early age and 12-14 layers of cells in adult animals. The mean thickness (µm) of corneal epithelium in group I to III animals was 117.04±3.37, 122.59±2.23 and 133.71±3.55 respectively. Bowman’s membrane of cornea was measured 12.97±0.86, 17.22±0.55 and 18.71±0.68 in group I, II and III animals respectively. The mean thickness (µm) of corneal stroma in group I to III animals was 600.75±10.33, 672.22±10.46 and 690.00±5.32 respectively. This was mainly due to progressive increase in number of collagenous lamellae. The keratocytes became thin in old animals. Thickness of posterior band zone (PBZ) of Descemet’s membrane was increased with advancement of age. The mean thickness (µm) of Descemet’s membrane and endothelium together was in group I, II and III animals was 16.48±0.91, 41.29±2.25 and 54.82±1.44 respectively. The corneal endothelial cells were enlarged and increased in size in old buffaloes. The age related pigmentation was also noticed in epithelium and stroma of cornea in animals of group III. Density of sub basal nerve plexuses between the stroma and the corneal epithelium was not altered with advancement of age.

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