The detection of Chlamydia trachomatis by direct Immunofluorescence in conjunctival smears from patients with trachoma and patients with ophthalmia neonatorum using a conjugated monoclonal antibody Mabey, D. C. W., and Booth-Mason, S. (Med. Res. Council Lab., Fajara, The Gambia). J. Hyg. 96:83, 1986

1986 ◽  
Vol 102 (2) ◽  
pp. 291
Keyword(s):  
Monoclonal Antibody ◽  
Chlamydia Trachomatis ◽  
The Gambia ◽  
Direct Immunofluorescence ◽  
Ophthalmia Neonatorum

scholarly journals The detection ofChlamydia trachomatisby direct immunofluorescence in conjunctival smears from patients with trachoma and patients with ophthalmia neonatorum using a conjugated monoclonal antibody

1986 ◽  
Vol 96 (1) ◽  
pp. 83-87 ◽  
Author(s):  
D. C. W. Mabey ◽  
S. Booth-Mason
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Chlamydia Trachomatis ◽  
Sensitivity And Specificity ◽  
Direct Immunofluorescence ◽  
Conjunctival Epithelium ◽  
Cotton Wool ◽  
Minimum Requirement ◽  
Ophthalmia Neonatorum ◽  
Immunofluorescent Technique

SUMMARYDuplicate specimens were taken with cotton-wool swabs from the upper tarsal conjunctiva of 63 patients living in Gambian villages in which trachoma is endemic and from 34 infants with ophthalmia neonatorum (ON) attending an outpatient clinic in The Gambia. The detection ofChlamydia trachomatisby direct immunofluorescence (IF) using a conjugated monoclonal antibody to its principal outer membrane protein was compared with isolation in cycloheximide-treated McCoy cells. For trachoma, the sensitivity and specificity of the immunofluorescent technique were 62% and 100% respectively if ten elementary bodies (EBs) was taken as the minimum requirement for positivity by IF. If all cases with one or more EB were considered positive, the sensitivity was 81% and the specificity 85%. For ON the sensitivity and specificity were 100% and 95% respectively, regardless of which criterion was used. In view of its simplicity and easy applicability to field conditions it seems likely that direct IF using monoclonal antibodies may be a useful technique for the detection ofC. trachomatisin the conjunctival epithelium of patients with trachoma.

scholarly journals Comparison of Direct Immunofluorescence and Giemsa Staining in Chlamydia trachomatis Follicular Conjunctivitis

2015 ◽  
Vol 17 (10) ◽  
Author(s):  
Fahimeh Asadi-Amoli ◽  
Zohreh Nozarian ◽  
Vahid Mehrtash ◽  
Hooshang Beheshtnejad ◽  
Avishan Shabani
Keyword(s):  
Chlamydia Trachomatis ◽  
Giemsa Staining ◽  
Direct Immunofluorescence ◽  
Follicular Conjunctivitis

Ophthalmia Neonatorum in Nairobi Kenya: The Roles of Neisseria gonorrhoeae and Chlamydia trachomatis

1986 ◽  
Vol 153 (5) ◽  
pp. 862-869 ◽  
Author(s):  
L. Fransen ◽  
H. Nsanze ◽  
V. Klauss ◽  
P. Van der Stuyft ◽  
L. D'Costa ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Ophthalmia Neonatorum

Prevalence of Urogenital Chlamydia Trachomatis Infection in El Salvador. II. Gynaecology Outpatients

1992 ◽  
Vol 3 (6) ◽  
pp. 434-436 ◽  
Author(s):  
Ana Berta Cañas Posada ◽  
Jon Jonasson ◽  
Leonor de Linares ◽  
Solgun Bygdeman
Keyword(s):  
Chlamydia Trachomatis ◽  
El Salvador ◽  
Direct Immunofluorescence ◽  
Beta Lactamase ◽  
Gray Zone ◽  
Chlamydia Trachomatis Infection ◽  
San Salvador ◽  
Urogenital Infection ◽  
Healthy Women ◽  
Chlamydial Antigen

The prevalence of urogenital infection caused by Chlamydia trachomatis was examined in 100 non-pregnant women with cervicitis, and 100 healthy women, in San Salvador City, El Salvador. Pharmacia Chlamydia EIA test was used for the detection of chlamydial antigen in urethral and cervical specimens from all the women. Direct immunofluorescence was used for confirmative tests on the EIA positive and the negative gray zone samples. C. trachomatis antigen was detected in 28% of the women with cervicitis compared with 5% in the group of healthy women ( P < 0.001). The cervicitis group were also screened for Neisseria gonorrhoeae which was isolated from 12% of them. One strain out of 12 was beta-lactamase producing (PPNG). Five per cent of the women with cervicitis had simultaneous C. trachomatis and N. gonorrhoeae infections.

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