scholarly journals Comparison of Direct Immunofluorescence and Giemsa Staining in Chlamydia trachomatis Follicular Conjunctivitis

2015 ◽  
Vol 17 (10) ◽  
Author(s):  
Fahimeh Asadi-Amoli ◽  
Zohreh Nozarian ◽  
Vahid Mehrtash ◽  
Hooshang Beheshtnejad ◽  
Avishan Shabani
Keyword(s):  
Chlamydia Trachomatis ◽  
Giemsa Staining ◽  
Direct Immunofluorescence ◽  
Follicular Conjunctivitis

Prevalence of Urogenital Chlamydia Trachomatis Infection in El Salvador. II. Gynaecology Outpatients

1992 ◽  
Vol 3 (6) ◽  
pp. 434-436 ◽  
Author(s):  
Ana Berta Cañas Posada ◽  
Jon Jonasson ◽  
Leonor de Linares ◽  
Solgun Bygdeman
Keyword(s):  
Chlamydia Trachomatis ◽  
El Salvador ◽  
Direct Immunofluorescence ◽  
Beta Lactamase ◽  
Gray Zone ◽  
Chlamydia Trachomatis Infection ◽  
San Salvador ◽  
Urogenital Infection ◽  
Healthy Women ◽  
Chlamydial Antigen

The prevalence of urogenital infection caused by Chlamydia trachomatis was examined in 100 non-pregnant women with cervicitis, and 100 healthy women, in San Salvador City, El Salvador. Pharmacia Chlamydia EIA test was used for the detection of chlamydial antigen in urethral and cervical specimens from all the women. Direct immunofluorescence was used for confirmative tests on the EIA positive and the negative gray zone samples. C. trachomatis antigen was detected in 28% of the women with cervicitis compared with 5% in the group of healthy women ( P < 0.001). The cervicitis group were also screened for Neisseria gonorrhoeae which was isolated from 12% of them. One strain out of 12 was beta-lactamase producing (PPNG). Five per cent of the women with cervicitis had simultaneous C. trachomatis and N. gonorrhoeae infections.

scholarly journals Chlamydia Trachomatis detection in different urogenital samples

2002 ◽  
Vol 51 (1) ◽  
pp. 95-100
Author(s):  
К. V. Shalepo ◽  
E. V. Shinitsyna ◽  
A. N. Savitsheva ◽  
M. Domeika
Keyword(s):  
Cell Culture ◽  
Chlamydia Trachomatis ◽  
Sensitivity And Specificity ◽  
Culture Method ◽  
Direct Immunofluorescence ◽  
Immunofluorescence Test ◽  
Female Urethra ◽  
Chain Reaction ◽  
Vaginal Swabs ◽  
Polymerase Chain

The results of Chlamydia trachomatis detection in different urogenital samples (vagina, cervix, urethra, urine) are presented in this report. The study was carried out for the period of 1999 to 2000. A total of 397 women and 253 men were examined. Cervical, urethral and vaginal swabs from women, and urethral, first voided urine (FVU) specimens from men were tested. For diagnosis of Chlamydia, trachomatis the following methods were used: polymerase chain reaction (PCR), direct immunofluorescence test (DIF) and cell culture (CC). In male samples, more often chlamydiae were detected in the urethra (11,6%), more rarely - in the FVU (6%). When female samples were tested, more often C. trachomatis was found in the vagina (18,4%), and less often - in the cervix (14. 4) and the urethra (8. 8%). The sensitivity and specificity of the methods used to test urogenital samples were determined. The PCR sensitivity and specificity was shown to be 75 and 100% for C. trachomatis detection in the cervix, 75 and 97. 5% - in the female urethra, and 63 and 99% - in the vagina, respectively. The PCR sensitivity and specificity was found to be 78 and 100% in the male urethral specimens and 100 and 99. 6% in the FVU, respectively. The sensitivity of cell culture method used for chlamydiae detection in cervical, female and male urethral samples was low - 33. 9, 47. 1 and 50% respectively. The CC specificity was 100%.

Vaginal Chlamydia trachomatis Prevalence in Sexually Abused Prepubertal Girls

PEDIATRICS ◽  
1987 ◽  
Vol 79 (2) ◽  
pp. 235-238 ◽  
Author(s):  
Carlos Daniel Fuster ◽  
Lawrence S. Neinstein
Keyword(s):  
Chlamydia Trachomatis ◽  
Sexually Abused ◽  
Immunofluorescence Assay ◽  
Culture Technique ◽  
Direct Immunofluorescence ◽  
Abused Children ◽  
Negative Results ◽  
Direct Immunofluorescence Assay ◽  
Sexually Abused Children ◽  
Prepubertal Girls

The prevalence rate of Chlamydia trachomatis in the vagina of prepubertal sexually abused children was examined. Additionally, the culture technique was compared to direct immunofluorescence assay (DFA) for diagnosing chlamydial infections in the vaginas of prepubertal children. The study group included 50 consecutive prepubertal girls examined for sexual abuse in an urban pediatric hospital's emergency room. Vaginal swabs were obtained for culture and direct immunofluorescence assay of C trachomatis and for gonorrhea culture. of these, 8/47 (17%) of the Chlamydia cultures, 4/43 (9.3%) of the direct immunofluorescence assay specimens, and 0/49 gonorrhea cultures were positive for Chlamydia. All patients with positive findings by direct immunofluorescence assay also had positive chlamydial cultures. Of the four other patients with positive cultures for Chlamydia, two had negative findings by direct immunofluorescence assay and two had inconclusive results reported. Twenty-nine patients had a syphilis serologic study performed, and all had negative results. There was no significant difference between the Chlamydia-positive and -negative groups with regards to age, race, nature of the abuse, frequency of abuse, and symptoms or findings on physical examination. Although not statistically significant, children with cultures positive for C trachomatis reported rectal penetration five times and vaginal penetration twice as often as those children with C trachomatis-negative results. This study demonstrates a significant prevalence of vaginal chlamydial infections in sexually abused children. Vaginal cultures for C trachomatis should be included in the medical evaluation of sexually abused girls. The culture technique was superior to the direct immunofluorescence assay in this group of patients.

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