The use of Coomassie Brilliant Blue G250 perchloric acid solution for staining in electrophoresis and isoelectric focusing on polyacrylamide gels

1975 ◽  
Vol 64 (2) ◽  
pp. 509-516 ◽  
Author(s):  
A.H. Reisner ◽  
Patty Nemes ◽  
Carolyn Bucholtz
Keyword(s):  
Acid Solution ◽  
Isoelectric Focusing ◽  
Perchloric Acid ◽  
Brilliant Blue ◽  
Polyacrylamide Gels ◽  
Perchloric Acid Solution ◽  
Coomassie Brilliant Blue ◽  
Coomassie Brilliant

scholarly journals Elution of proteins from polyacrylamide eels: a simple and economic procedure

1989 ◽  
Vol 31 (1) ◽  
pp. 44-47
Author(s):  
Vera Bongertz
Keyword(s):  
Gel Electrophoresis ◽  
Brilliant Blue ◽  
Polyacrylamide Gels ◽  
Coomassie Brilliant Blue ◽  
Coomassie Brilliant

A simplified methodology for the quantitative electroelution of proteins from polyacrylamide gels is described. After staining with Coomassie Brilliant Blue R 250, the identified bands are excised from the gel and the proteins eluted using a procedure developed for use in conventional tube gel electrophoresis equipment.

MEASUREMENT OF CANINE PITUITARY PROLACTIN

1973 ◽  
Vol 56 (2) ◽  
pp. 245-NP ◽  
Author(s):  
P. G. SALUJA ◽  
M. GRONOW ◽  
J. M. HAMILTON
Keyword(s):  
Isoelectric Focusing ◽  
Isoelectric Point ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Brilliant Blue ◽  
Coomassie Brilliant Blue ◽  
Ovine Prolactin ◽  
Pituitary Prolactin ◽  
Coomassie Brilliant

SUMMARY Isoelectric focusing in polyacrylamide gel followed by staining with Coomassie brilliant blue was used for the densitometric estimation of ovine prolactin standard and canine pituitary prolactin. The results were compared with those obtained by conventional polyacrylamide gel electrophoretic procedures and isoelectric focusing was found to be a valid technique for the estimation of prolactin and to offer greater technical convenience. The mobility of ovine and canine prolactin was similar in isoelectric focusing and gel electrophoresis. The isoelectric point of ovine and canine prolactin was found to be 6·17 and 6·61 respectively. Constant levels of prolactin were found in the pituitaries of bitches at oestrus.

Coomassie-Brilliant Blue Staining of Polyacrylamide Gels

2012 ◽  
pp. 465-469 ◽  
Author(s):  
Claudia Arndt ◽  
Stefanie Koristka ◽  
Anja Feldmann ◽  
Holger Bartsch ◽  
Michael Bachmann
Keyword(s):  
Brilliant Blue ◽  
Blue Staining ◽  
Polyacrylamide Gels ◽  
Coomassie Brilliant Blue ◽  
Coomassie Brilliant Blue Staining ◽  
Coomassie Brilliant

scholarly journals Effect of pre-stain methanol washing on the sensitivity of CBBR-250 staining in SDS-polyacrylamide gels

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Rashid Saleem ◽  
Absar-ul Hasnain ◽  
Riaz Ahmad
Keyword(s):  
Molecular Weight ◽  
Acetic Acid ◽  
Staining Method ◽  
Muscle Protein ◽  
Brilliant Blue ◽  
Low Molecular Weight ◽  
Polyacrylamide Gels ◽  
Coomassie Brilliant Blue ◽  
Protein Research ◽  
Coomassie Brilliant

AbstractCoomassie Brilliant Blue R-250 (CBBR-250) is the most frequently used dye stain in protein research. However, relatively poor sensitivity and long application durations are the major limitations of this stain. In the present study, we have investigated the effect of pre-stain methanol washing on the sensitivity of the conventional CBB staining method. Concentrations of methanol ranging from 5 to 30% were prepared in 5% acetic acid. Pre-stain washing of SDS-gels using acetic acid (5%) and methanol were assessed at various timings and temperatures. Our results demonstrate that pre-stain washing by methanol greatly improves the sensitivity of the CBB stain and facilitates the visualization of low molecular weight components present in a complex muscle protein, actomyosin. The outcome of this study also revealed duration of pre-stain washing and temperature as other factors that determine the sensitivity of CBB staining method. Therefore, it is suggested that pre-stain washing with methanol may improve the sensitivity of CBB stain in SDS-PA gels.

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