Abstract
The study aimed at the determination of dynamic relationship between mineralisation processes and antioxidative/oxidative status during the development of osteopenia. One hundred and two healthy female Wistar rats at the age of 2 months and initial body weight of 200 g were used in the experiment. The rats were divided into control (CON, n=6), sham operated (SHO, n=48), and ovariectomised (OVX, n=48) groups. Animals from SHO (n=6) and OVX (n=6) groups were sacrificed every week during 8 weeks of the experiment in order to detect dynamic changes in examined parameters. The samples were collected weekly from day 7 to day 56. The femora were examined with the use of DXA (bone mineral density) and pQCT (area, mineral content, volumetric density of trabecular and cortical part of distal femora). The pQCT scans were performed 5 mm from distal end of the tibia. The determination of activity of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in haemolysates of erythrocytes were performed spectrophotometrically. Obtained data showed wave like changes in both enzyme activities and bone parameters and indicated the importance of the 2nd-3rd and 5th-6th week after surgery as a key moment for bone metabolism and activity of enzymatic antioxidative defence during the development of osteopenia induced by bilateral ovariectomy. The obtained results proved that alterations in activity of GSH-Px and SOD, and pQCT ahead the changes registered by DXA by 7 d.
An Improved Spectrophotometric Procedure for the Determination of Urinary Metanephrines
