Comparative Study of Local and Imported Enzymes in Improving the Nutritional Values of Agricultural Waste on Performance and Economic Efficiency of Iraqi Lambs

In the current study, we use 25 local Iraqi lambs. With initial body weight (24) kg. The lambs were randomly divided into five treated groups, the first group without treatment as control, the second and third treated with 20%and 40% Agricultural Waste treated with trade enzymes, fourth and fifth treatment 20%and 40% Agricultural Waste treated with local enzymes. The result demonstrated a significant (P<0.01) increase in the quantity of roughages, total feed intake, Neutral Detergent Fiber (NDF) and Acid Detergent Fiber( ADF) and Lignin (ADL) in T5 followed by T4 compared with others treated groups and showed a significant (P<0.01) increase in the digestibility of NDF ,ADF in T4, T5 compare with other treated groups. While T5and T4 recorded Superiority increase in weight gain, and significant improvement in economic cost efficiency with ratio improvement of 5.90, 5.97 % respectively

Replacement of Astaxanthin With Lutein in Diets of Juvenile Litopenaeus vannamei: Effects on Growth Performance, Antioxidant Capacity, and Immune Response

An 8-week feeding experiment was conducted to investigate whether diet supplementation of lutein could result in similar growth performance, antioxidant capacity, and immunity of Litopenaeus vannamei when compared to dietary astaxanthin. Juvenile L. vannamei (initial body weight: 0.64 ± 0.04 g) were fed with one of five isonitrogen and isolipids diets with/without lutein or astaxanthin [control group (C); the lutein (L) groups contained 0, 62.5, 75, 87.5 ppm lutein, respectively, the astaxanthin (A) group contained 50 ppm astaxanthin]. Results showed that dietary supplementation of lutein ranging from 62.5 to 75 ppm resulted in similar growth performance (WGR, SGR, FCR, and SR) of L. vannamei compared with the A group (P &gt; 0.05). Apart from that, no statistical difference was observed in antioxidant parameters (hemolymph T-AOC, hemolymph MDA, and RNA expression level of GSH-PX, CAT), anti-inflammatory ability (Relish, Rho, and HSP70) and apoptosis-related gene expression (Caspase3) among lutein treatments ranging from 62.5 to 87.5 ppm and the A group (P &gt; 0.05). These results indicate that a dose of 62.5–75 ppm of lutein was suitable in the diet of L. vannamei for substituting dietary astaxanthin.

Efficacy of Clinically Used Anthelmintics Against Toxocariasis of Buffalo Calves in Bangladesh

Toxocariasis is a paramount parasitic disease having over 50% prevalence among newborn buffalo calves in Bangladesh. The objective of this study was to compare the efficacy of clinically used anthelmintics and their subsequent effects on the hematological parameters and body weight in buffalo calves in commercial buffalo farms in coastal region. Thirty-two buffalo calves below 3 months of age with clinical Toxocara infection had been selected and treated with albendazole (ABZ), levamisole (LVM) and ivermectin (IVM). The EPG counts, hematological parameters and initial body weight of buffalo calves were recorded on the day of anthelmintic treatment (day 0). Fecal samples were collected on day 14 and 28 including hematological parameters and body weight were recorded on day 28 post-treatment. The efficacy (%) of anthelmintics were estimated by fecal egg count reduction test (FECRT). The parameters like Hb, PCV, ESR, TEC, TLC were analyzed from the blood samples. The FECRT revealed 96.83% efficacy for IVM followed by 94.23% and 85.84% for LVM and ABZ, respectively. Anthelmintic treated calves showed significant (p<0.01) increase in Hb, PCV, TEC and body weight, and decrease in ESR and TLC as a result of worm expulsion from buffalo calves after 28 days of post-treatment. Among the tested anthelmintics, IVM was found to be more effective against toxocariasis in buffalo calves. This is a novel new information on anthelmintics efficacy in buffalo calves in Bangladesh. Further studies are imperative to explore the factors responsible for high prevalence of this deadly nematode in buffalo calves.

Can Weight Watchers (WW) Help Address Maternal Obesity? An Audit of Weight Change in Women of Childbearing Age and Mothers-To-Be, Referred into a Commercial Slimming Programme

Objectives The scale of overweight and obesity amongst women of childbearing age or mothers to be, living in Wales, places a considerable burden on the NHS and public health. High BMI (over 30) during pregnancy increases the health risks for mother and baby. Policy advice recommends weight management services are available to help women lose weight before and whilst planning pregnancy. In parts of Wales, NHS partnerships with commercial companies provide weight management services for women considering or planning pregnancy. This study evaluates whether an established referral Weight Watchers (WW) programme, known to be effective in adults in England, can help mothers-to-be living in North Wales lose weight. Methods Analysis used routine data from 82 referrals to WW between June 2013 and January 2015. Participants received a referral letter inviting them to attend face-to-face group workshops combined with a digital experience. The programme encompassed healthy eating, physical activity and positive mind-set. Trained WW staff measured bodyweight before, during and at 12 weeks. On entry to the course, participants had a median age of 31.4 years (interquartile range (IQR) 28–34) with a median BMI of 36.8 kg/m2 (IQR 33.3–43.7). Results Women completing the course (n = 34) had a median weight loss of 5.65 kg (IQR 0.45–10.85), equating to 5.7% (SD 3.46) of initial body weight. Intention-to-treat analysis (last observation carried forward), which included lapsed courses n = 66, showed a median weight loss of 3.6 kg (IQR − 2.53 to 9.73), equating to 3.7% (SD 3.62) of initial body weight. Overall, there was significant weight loss during the WW programme (Wilcoxon signed rank test Z = − 6.16; p < 0.001). Weight loss was significantly correlated with the number of workshops attended (Spearman correlation coefficient 0.61 p < 0.001). The proportion of all 82 participants (intention to treat, baseline observation carried forward) that achieved a weight loss of ≥ 5% initial weight was 30.5%. Conclusions for Practice Referral of obese mothers-to-be into WW can successfully achieve short-term weight loss, at or above 5%, in approximately one third of participants. The dose–response effect supports a causal inference. Successful weight loss at this critical life stage may provide women with the necessary motivation to initiate weight loss for healthy pregnancy, however further research is required.

The effect of probiotic and prebiotic-MOS on the survival, growth and immune competence on spiny lobster Panulirus homarus culture

The use of probiotics and prebiotic to improve growth and health status of spiny lobster has been evaluated. The purpose of this study was to obtain the effect of probiotics and prebiotics for the culture of spiny lobster Panulirus homarus. The method was initiated with culturing of probiotics (4 strains) and supplemented it in a moist diets. Spiny lobster was collected from Jembrana-Bali waters with an initial body weight of 70.34 ± 4.5 g and cultured in concrete tanks with volume of 4 m3. Initial stock density for each treatment was 15 pcs / m3. The treatments tested were supplemented on moist diets with (A) probiotics, (B) probiotics and prebiotics-MOS (Mannan Oligo Sacharida) and (C) Controle (without probiotics and prebiotic) and each treatment with three replication. The results obtained that the survival rate of spiny lobster was not significantly different (P> 0.05), i.e. (A) 92.70%, (B) 93.33 (%) and (C) 93.33% respectively. However, the results of probiotics supplementation as well as a combination of probiotics and prebiotics showed growth differences compared to control (P<0.05), namely (A) 156.97 ± 6.17 g, (B) 153.75 ± 9.17 g, while (C) control 131.47 ± 7.91 g. The probiotics supplementation on moist diets could increased health status of spiny lobsters, this was expressed by target genes related to immunity (ALFHa-1, ALFHa-4, SAA, ProPO, Tgase and CP). Spiny lobster immunity increased by 2.60 to 42.7 times after challenging with MHD (Milky Haemolymph Disease). The supplementation of probiotics and prebiotics (MOS) could increase immune response by 2.10 to 25.75 times, respectively after challenging with MHD.

PSVI-12 The effects of road transportation with or without homeopathic remedy supplementation on growth performance, nutrient digestibility, microbiota, serum cortisol and superoxide dismutase in growing pigs

This study investigated the effects of road transportation with or without homeopathic remedy (Convermax) supplementation on growth performance, nutrient digestibility, fecal microbiota, and serum cortisol and superoxide dismutase (SOD) concentrations in growing pigs. A total of 180 49-day old growing pigs [(Yorkshire × Landrace) × Duroc] with an initial body weight of 13.17 ± 0.02 kg were randomly allotted to 2 groups based on the initial body weight, containing 18 replicates with 5 pigs (mixed sex) in each. The pigs were fed dietary supplementation of a homeopathic remedy (Convermax) (0 or 200 mg/kg of feed, as-fed) for 35 days. On day 21, forty-five pigs (70-day old; 25.25 ± 0.37 kg) were randomly selected from each group and assigned to either 2 h of road transportation or no road transportation, resulting in a 2×2 factorial design. Results showed that road transportation led to an increase in the fecal coliform bacteria counts (P = 0.023) and serum cortisol concentration (P = 0.039) and a decrease in the serum superoxide dismutase (SOD) concentration (P &lt; 0.001). However, supplementing homeopathic remedy (Convermax) to the diet of growing pigs increased gain to feed ratio (P = 0.042), apparent nitrogen digestibility (P = 0.019), and serum SOD concentration (P = 0.007), whereas decreased serum cortisol concentration (P = 0.022). In brief, road transportation induced stress response for growing pigs and increased harmful bacteria counts in their intestines. Dietary supplementation of homeopathic remedy (Convermax) alleviated stress response, improved apparent nitrogen digestibility, and increased gain to feed ratio. However, no significant interactive effects between road transportation with or without dietary homeopathic remedy (Convermax) levels were observed on the detected parameters in growing pigs.

Health concerns of lambs fed cottonseed hulls combined with chitosan by examining the blood metabolic profile and histopathology of the kidney, liver, and rumen

This study analysed the effect of cottonseed hulls and chitosan in diets for lambs by determining the blood metabolite profile, and the histopathology of the kidney, liver, and rumen. Eighty non-castrated Santa Inês lambs, approximately 120 days of age and a mean initial body weight (b.w.) of 22.6 (standard deviation ± 2.2 kg) were assigned to a completely randomised design, with a 2 × 2 factorial arrangement. Two chitosan levels and two cottonseed forms were evaluated. The experimental diets were following: diet containing whole cottonseed hulls (WC) without the addition of chitosan; diet containing WC with 136 mg/kg b.w. chitosan added; diet containing ground cottonseed hulls (GC) without the addition of chitosan; diet containing GC with 136 mg/kg b.w. chitosan added. The blood metabolites and hepatic enzymes ALT, AST, GGT were not significantly influenced in the treatment groups, except for the serum cholesterol concentration which was lower (P &lt; 0.05) when the chitosan was combined with the whole cottonseed hulls. There were histopathological alterations (P &lt; 0.05) in the liver and kidney tissue and moderate changes in the rumen samples in the animals fed cottonseed without chitosan, however, when chitosan was added, the changes were less marked. The combination of chitosan with cottonseed hulls (ground or whole) can be supplied safely to feedlot finishing lambs without compromising their health.

Heat processing increased the digestibility of phosphorus in soybean expeller, canola meal, and canola expeller fed to growing pigs

It is hypothesized that heat processing may increase P digestibility in different protein sources fed to growing pigs. A study was conducted to determine the apparent total tract digestibility (ATTD) and standardized total tract digestibility (STTD) of P in soybean expeller (SBE) produced from oil extraction using dry extrusion and expelling and to investigate the effects of heat treatment on the ATTD and STTD of P in SBE, canola meal (CM), and canola expeller (CE) fed to growing pigs. Thirty-six growing barrows with an initial body weight of 19.0 ± 1.0 kg (mean ± SD) were assigned to 1 of 6 experimental diets in a completely randomized design to give 6 replicates per diet. The experimental design was a 3 × 2 factorial arrangement including three oilseed meals with or without heat treatment. The diets were formulated to contain non-autoclaved or autoclaved (at 121°C for 60 min) SBE, CM, and CE as the sole source of P. Limestone was included in diets to maintain a Ca:total P ratio of 1.3:1 across diets. Pigs were individually housed in metabolism crates for 12 days, including 7 days for adaptation and 5 days for total collection of feces. Pigs were offered their daily ration at 2.8 times their maintenance energy requirement. Data were analyzed using the PROC MIXED of SAS. Heat treatment increased (P &lt; 0.05) the ATTD and STTD of P. Pigs fed the SBE diets had greater (P &lt; 0.05) ATTD and STTD of P than pigs fed CM and CE diets. For the autoclaved ingredients, the values of STTD of P were 49.4, 23.2, and 25.8% for SBE, CM, and CE, respectively, whereas STTD of P in non-autoclaved SBE, CM, and CE were 48.5, 20.2, and 22.5%. Heat treatment increased (P &lt; 0.05) the ATTD of Ca. In conclusion, heat treatment increased ATTD and STTD of P and ATTD of Ca in SBE, CM, and CE fed to growing pigs. The ATTD and STTD of P in SBE determined in the current study were 41.0 and 48.5%, respectively.

Structural state of diaphysis of humeral bone after implantation of hydroxylapatite into tibia and oral administration of calcium medications

BACKGROUND: Osteoporosis and osteopenias are the most common metabolic diseases of skeleton leading to loss of bone mass, which in turn contributes to increase in the incidence of fractures occur against their background. To note, bone fractures often require not only correction of phosphorus-calcium metabolism, but also bone-plastic operations. AIM: To investigate the structure of the middiaphysis of the humeral bone after plastic surgery of the tibia defect with OK-015 material and substantiate the possibilities of correction of its damages by “Biomin MK”. MATERIALS AND METHODS: The experiment involved 210 male laboratory rats with initial body weight from 135 to 145 g: group A — control, in group B perforation of the tibia, in group C filling of the defect with OK-015 material. In the group D after perforation and in the group E after implantation, “Biomin MK” was used intragastrically through a tube at a dose of 90 mg/kg/day. RESULTS: Perforation of the tibia was accompanied by an increase in resorptive processes and inhibition of bone formation in the diaphysis of the humeral bone with maximum deviations by the 30th day after surgery. The implantation of the OK-015 material was accompanied by aggravation of the identified alterations within 15 days, but in the later period, the structure of the middiaphysis recovered faster. With intragastric administration of “Biomin MK” through a tube after the bone plasty, the structure of the middiaphysis of the humeral bones showed more evident changes by the 7th and 15th day, but by the 30th and 60th day after the implantation, the thickness of the layer of the external circumferential lamellae was greater than in group C by the 3.77% and 4.27%, and the thickness of the internal circumferential lamellae and the diameter of osteons by the 60th day — by 3.51%, 6.46% and 18.64%. CONCLUSIONS: The administration of “Biomin MK” during implantation of OK-015 material into the tibia is accompanied by the restoration of the structure of the middiaphysis of the humeral bones, mainly from the 30th to 60th days after the surgery.

Clinical and Pathogenetic Assessment of Relationships Between the Dynamics of Body Weight Changes and Atrial Fibrillation in Patients with Primary Obesity

Aims. To evaluate the impact of body weight dynamics on the clinical course of atrial fibrillation in obese patients.Materials and methods. The study included 101 primary obese patients with paroxysmal or persistent atrial fibrillation. Study design: a retrospective, single-center, comparative study. Retrospectively аccording to the he body weight dynamics, patients were divided into 3 groups: those who increased their body weight by >3 % (Group 1, n=40), maintained their initial body weight by ±2.9 % (Group 2, n=29), and reduced their initial body weight by >3 % (Group 3, n=32). Follow-up examinations by a doctor were carried out at least once every 6 months for minimum 36 months. Change in AF type was determined by disease patterns and 7-day Holter monitoring results. The groups were comparable in gender (p=0,9267), age (p=0,3841), height (p=0,8900), and disease form (Paroxysmal atrial fibrillation /Persistent atrial fibrillation) (p=0,8826), the severity of symptoms on the European Heart Rhythm Association score of atrial fibrillations (p=0,8687) and systolic blood pressure at the beginning of the study (p=0,4500).Results. At the final control examination, the body weight of patients in Group 1 increased by an average of 11,4 [9,3; 13,1] kg (р <0,001*), while weight loss in Group 3 averaged -6,2 [-8,4; -5,3] kg (p <0,001*). The decrease in body weight of Group 2 patients was insignificant (p=0,5377) and amounted to -0,1 [-2,0; 1,3] kg. The progression of the disease from paroxysmal to persistent form was observed among 15 (37 %) patients in Group 1, 9 (31 %) patients — in Group 2, 2 (6 %) patients — in Group 3 (p=0,0079*). The regression of arrhythmia from persistent to paroxysmal form was not registered in group 1 (0 %), in group 2, the reverse development of the disease was noted in 1 patient (3 %) and in group 3 — in 6 patients (19 %) (p=0,0053*). There were no free from AF patients in Group 1 at the final follow-up, while 2 (7 %) patients were free from AF in Group 2 and 7 (22 %) — in Group 3 (р=0,0047*). In patients undergoing ablation, procedural success was determined after a 3-month blind period. The need for interventional procedures to restore the sinus rhythm and their multiplicity when comparing the groups did not differ significantly. However, in a pairwise comparison, the difference between groups 1 and 3 of participants was statistically significant (p=0,0079* and p=0,0374*, respectively). Conclusion. This study demonstrates the relationships between the dynamics of body weight and the clinical course of atrial fibrillation. The progression of obesity leads to the progression of the disease. Weight-loss management reverses the type and natural progression of AF, improves the prognosis and the course of disease, regardless of other significant risk factors, increases the anti-arrhythmic therapy effect and the effect of interventional treatment.