The stimulation of the hexosemonophosphate shunt and glutathione reduction by chlorpromazine in the presence of a subcellular, particulate fraction of guinea pig brain

We have used the adenosine-stimulated adenylate cyclase of guinea-pig brain to examine the potency of diazepam as an adenosine uptake inhibitor. Diazepam at concentrations in the range 10–500 μM stimulates the production of cAMP in incubated slices of guinea-pig cerebral cortex, with maximal fivefold stimulations over basal levels by 200 μM diazepam. The increases can be largely (but not completely) blocked by the adenosine antagonist theophylline or by addition of excess adenosine deaminase to the system. It appears that the stimulation of cAMP production is due to a blockade of adenosine uptake which results in an increase in extracellular adenosine and concomitant activation of the adenosine receptor coupled to adenylate cyclase. Since the cAMP response to standard adenosine uptake blockers (dipyridamole, dilazep) can be completely blocked by theophylline or adenosine deaminase, a small component of the diazepam response cannot be explained by an adenosine effect. The concentration of diazepam at which the first significant cAMP increase occurs is 10 μM or slightly lower. This is significantly higher than the concentration of diazepam needed to saturate the pharmacologically characterized central nervous system receptors for benzodiazepines.

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Stimulation of the production of unesterified fatty acids in nerve endings of guinea-pig brain in vitro by noradrenaline and 5-hydroxytryptamine

Biochemical Journal ◽

10.1042/bj1260575 ◽

1972 ◽

Vol 126 (3) ◽

pp. 575-585 ◽

Cited By ~ 17

Author(s):

C. J. Price ◽

C. E. Rowe

Keyword(s):

Fatty Acids ◽

Cerebral Cortex ◽

Guinea Pig ◽

Synaptic Vesicles ◽

Nerve Endings ◽

Synaptic Membranes ◽

Pig Brain ◽

Guinea Pig Brain ◽

Stimulation Of

1. Noradrenaline (1mm) and 5-hydroxytryptamine (1mm) stimulated the production of unesterified palmitate, oleate, stearate and arachidonate in nerve endings (synaptosomes) isolated from combined guinea-pig cerebral cortex and cerebellum. 2. Iproniazid phosphate (0.36mm) increased the concentrations of the same acids in osmotically ruptured synaptosomes. Further addition of 1mm-noradrenaline or 1mm-5-hydroxytryptamine reversed this increase. 3. Noradrenaline (0.01mm) stimulated the production of unesterified fatty acids in isolated synaptic membranes. 5-Hydroxytryptamine (0.01mm) stimulated the production of unesterified fatty acids in synaptic membranes and synaptic vesicles.

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Entorhinal cortex long-term potentiation evoked by theta-patterned stimulation of associative fibers in the isolated in vitro guinea pig brain

Brain Research ◽

10.1016/0006-8993(93)91391-5 ◽

1993 ◽

Vol 600 (2) ◽

pp. 327-330 ◽

Cited By ~ 27

Author(s):

Marco de Curtis ◽

Rodolfo R. Llinas

Keyword(s):

Guinea Pig ◽

Entorhinal Cortex ◽

Long Term Potentiation ◽

Pig Brain ◽

Term Potentiation ◽

Guinea Pig Brain ◽

Stimulation Of

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Stimulation of guinea-pig brain adenylate cyclase by adenosine analogues with potent pharmacological activity

Life Sciences ◽

10.1016/0024-3205(80)90254-4 ◽

1980 ◽

Vol 26 (13) ◽

pp. 1079-1088 ◽

Cited By ~ 33

Author(s):

Les P. Davies ◽

Kenneth M. Taylor ◽

Richard P. Gregson ◽

Ronald J. Quinn

Keyword(s):

Adenylate Cyclase ◽

Guinea Pig ◽

Pharmacological Activity ◽

Adenosine Analogues ◽

Pig Brain ◽

Guinea Pig Brain ◽

Stimulation Of

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Communication calls produced by electrical stimulation of four structures in the guinea pig brain

PLoS ONE ◽

10.1371/journal.pone.0194091 ◽

2018 ◽

Vol 13 (3) ◽

pp. e0194091 ◽

Cited By ~ 4

Author(s):

David B. Green ◽

Trevor M. Shackleton ◽

Jasmine M. S. Grimsley ◽

Oliver Zobay ◽

Alan R. Palmer ◽

...

Keyword(s):

Electrical Stimulation ◽

Guinea Pig ◽

Pig Brain ◽

Communication Calls ◽

Guinea Pig Brain ◽

Stimulation Of

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Stimulation of Phospholipase A2 of Synaptosomes of Guinea-Pig Brain by Noradrenaline and other Amines

Biochemical Society Transactions ◽

10.1042/bst0010849 ◽

1973 ◽

Vol 1 (4) ◽

pp. 849-849 ◽

Cited By ~ 3

Author(s):

R. J. GULLIS ◽

C. E. ROWE

Keyword(s):

Guinea Pig ◽

Phospholipase A2 ◽

Pig Brain ◽

Guinea Pig Brain ◽

Stimulation Of

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Distribution of the Olfactory Fiber Input Into the Olfactory Tubercle of the In Vitro Isolated Guinea Pig Brain

Journal of Neurophysiology ◽

10.1152/jn.90792.2008 ◽

2009 ◽

Vol 101 (3) ◽

pp. 1613-1619 ◽

Cited By ~ 19

Author(s):

Giovanni Carriero ◽

Laura Uva ◽

Vadym Gnatkovsky ◽

Marco de Curtis

Keyword(s):

Guinea Pig ◽

Piriform Cortex ◽

Field Potential ◽

Lateral Part ◽

Olfactory Tubercle ◽

Olfactory Tract ◽

Pig Brain ◽

Guinea Pig Brain ◽

Stimulation Of

The olfactory tubercle (OT) is a cortical component of the olfactory system involved in reward mechanisms of drug abuse. This region covers an extensive part of the rostral ventral cerebrum and is relatively poorly studied. The intrinsic network interactions evoked by olfactory input are analyzed in the OT of the in vitro isolated guinea pig brain by means of field potential analysis and optical imaging of voltage-sensitive signals. Stimulation of the lateral olfactory tract induces a monosynaptic response that progressively decreases in amplitude from lateral to medial. The monosynaptic input induces a disynaptic response that is proportionally larger in the medial portion of the OT. Direct stimulation of the piriform cortex and subsequent lesion of this pathway showed the existence of an associative disynaptic projection from the anterior part of the piriform cortex to the lateral part of the OT that integrates with the component mediated by the local intra-OT collaterals. Optical and electrophysiological recordings of the signals evoked by stimulation of the olfactory tract during arterial perfusion with the voltage-sensitive dye di-2-ANEPEQ confirmed the pattern of distribution of the mono and disynaptic responses in the OT. Finally, current source density analysis of laminar profiles recorded with 16-channel silicon probes confirmed that the monosynaptic and disynaptic potentials localize in the most superficial and the deep portions of the plexiform layer I, as suggested by previous reports. This study sets the standard for further analysis of the modulation of network properties in this largely unexplored brain region.

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