Two distinct S-adenosyl-l-methionine:3,4-Dihydric phenol 3-O-methyltransferases of phenylpropanoid metabolism in soybean cell suspension cultures

1976 ◽  
Vol 173 (1) ◽  
pp. 301-305 ◽  
Author(s):  
Jonathan Poulton ◽  
Hans Grisebach ◽  
Jürgen Ebel ◽  
Bärbel Schaller-Hekeler ◽  
Klaus Hahlbrock
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Phenylpropanoid Metabolism ◽  
Dihydric Phenol ◽  
Soybean Cell

Coordinated changes in enzyme activities of phenylpropanoid metabolism during the growth of soybean cell suspension cultures

1974 ◽  
Vol 362 (3) ◽  
pp. 417-424 ◽  
Author(s):  
Jürgen Ebel ◽  
Bärbel Schaller-Hekeler ◽  
Karl-Heinz Kobloch ◽  
Eckard Wellman ◽  
Hans Grisebach ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Enzyme Activities ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Phenylpropanoid Metabolism ◽  
Soybean Cell

Metabolism of the Herbicide Isoproturon in Wheat and Soybean Cell Suspension Cultures

1999 ◽  
Vol 63 (2) ◽  
pp. 97-113 ◽  
Author(s):  
Werner Edgar Gläβgen ◽  
Dieter Komoβa ◽  
Olaf Bohnenkämper ◽  
Matthias Haas ◽  
Norbert Hertkorn ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Soybean Cell

Plant Metabolism Of Xenobiotics. Comparison Of The Metabolism Of 3.4-Dichloroaniline In Soybean Excised Leaves And Soybean Cell Suspension Cultures

1992 ◽  
Vol 47 (11-12) ◽  
pp. 823-829 ◽  
Author(s):  
Christine Gareis ◽  
Christiane Rivero ◽  
Ingolf Schuphan ◽  
Burkhard Schmidt
Keyword(s):  
Cell Suspension ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Wall Structures ◽  
End Products ◽  
Glycine Max L ◽  
Metabolism Of Xenobiotics ◽  
Soybean Cell ◽  
Detoxification Pathways

Excised leaves and cell suspension cultures of soybean (Glycine max L.) were incubated with [UL-14C]-3,4-dichloroaniline. The compound was almost completely metabolized after 48 h in both systems; it was apparent that the major detoxification pathways present in the excised leaves were also present in the cultured cells. Besides considerable amounts of insoluble residues, the N-glucosyl and the N-malonyl conjugates of 3,4-dichloroaniline, and a yet unknown metabolite was formed in the excised leaves; tentatively the latter was identified with the 6′-O-malonylester of N-glucosyl-3,4-dichloroaniline. The cell suspension cultures produced predominantly the N-malonyl conjugate, besides negligible amounts of the N-glucosyl conjugate, and insoluble residues; the majority of the N-malonyl compound was excreted into the medium. It was shown, that the metabolism of 3,4-dichloroaniline in excised leaves and cell suspension cultures of soybean was directed towards different end products: the excised leaves were able to make extensive use of cell wall structures as a deposit for xenobiotic bound residues, resembling plants in this respect; lack of these structures in the cultured soybean cells resulted in a soluble end product, namely the N-malonyl conjugate

Purification and Properties of Chalcone Synthase from Cell Suspension Cultures of Soybean

1987 ◽  
Vol 42 (11-12) ◽  
pp. 1200-1206 ◽  
Author(s):  
Roland Welle ◽  
Hans Grisebach
Keyword(s):  
Cell Suspension ◽  
Chalcone Synthase ◽  
Specific Activity ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Major Band ◽  
Apparent Homogeneity ◽  
Step Procedure ◽  
Purification And Properties ◽  
Soybean Cell

Incubation of soybean cell suspension cultures in B-5 medium containing 0.4 ᴍ sucrose caused induction of chalcone synthase (CHS) up to a specific activity of 8 µkat/kg. CHS from these cultures was purified to apparent homogeneity by a 5-step procedure. Isoelectric focussing of pure CHS gave a major band at pH 5.45 and two weaker bands at pH 5.35 and 5.5. At least the bands at pH 5.35 and 5.45 had CHS activity. Analysis of pure CHS by two-dimensional electrophoresis gave a set ot six proteins with a M, of 40 kDa and pIs between 6.0 and 6.6. One-dimensional PAGE of CHS under non-denaturing conditions gave three closely spaced protein bands. A specific antibody was raised against soybean CHS which cross-reacted with parsley CHS. Attempts to find synthesis of deoxychalconc or of the corresponding 7.4′-dihydroxyflavanonc with CHS of different purification stages and with various cofactors failed.

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