A reconstitution assay for the guanine nucleotide regulatory protein of the adenylate cyclase system using turkey erythrocyte membranes as the acceptor preparation

1982 ◽  
Vol 217 (1) ◽  
pp. 368-375 ◽  
Author(s):  
Gary L. Stiles ◽  
Robert J. Lefkowitz
Keyword(s):  
Adenylate Cyclase ◽  
Regulatory Protein ◽  
Erythrocyte Membranes ◽  
Adenylate Cyclase System ◽  
Guanine Nucleotide ◽  
Guanine Nucleotide Regulatory Protein

scholarly journals Restoration of guanine nucleotide- and fluoride-stimulated activity to an adenylate cyclase-deficient cell line with affinity-purified guanine nucleotide regulatory protein

1980 ◽  
Vol 190 (2) ◽  
pp. 439-443 ◽  
Author(s):  
T B Nielsen ◽  
R W Downs ◽  
A M Spiegel
Keyword(s):  
Adenylate Cyclase ◽  
Affinity Chromatography ◽  
Cell Line ◽  
Mutant Cell ◽  
Regulatory Protein ◽  
Erythrocyte Membranes ◽  
Guanine Nucleotide ◽  
Guanine Nucleotide Binding Protein ◽  
Guanine Nucleotide Binding ◽  
Guanine Nucleotide Regulatory Protein

A guanine nucleotide-binding protein purified from turkey erythrocytes by affinity chromatography confers both F— and guanine nucleotide-stimulation of adenylate cyclase to membranes from CYC- cells, a mutant cell line deficient in these responses. Interaction of turkey erythrocyte membranes with beta-adrenergic agonists before affinity chromatography, which is essential for binding of the guanine nucleotide regulatory protein to the affinity matrix, was also required for recovery of F—stimulation restoring activity in the affinity eluate.

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