Characterization of a temperature-sensitive membrane alteration in chick embryo fibroblasts infected with a temperature-sensitive mutant of Rous sarcoma virus

Aggregation capacity of chicken embryo chondrocytes decreases when transformed by Rous sarcoma viruses. Cell-to-cell aggregation capacity of chondrocytes infected with a T class temperature-sensitive mutant (tsNY68) (with the temperature-sensitive lesion at the src gene) of Rous sarcoma virus is dependent upon the temperature at which these cells are grown. When grown at the permissive temperature (36 degrees C), where the transforming gene is expressed, aggregation capacity was lower than normal while infected cells grown at the non-permissive temperature (41.5 degrees C) had similar capacity to aggregate to that of normal chondrocytes. However, after a prolonged period of culture (10 days), chondrocytes transformed by wild type SR-RSV regained the normal level of aggregation capacity. Cells transformed by tsNY68 and incubated at the permissive temperature for 10 days also regained the normal level of aggregation capacity. It appears therefore that RSV-transformed chondrocytes first become less adhesive but during long-term cultivation they regain their property to aggregate. The decrease of aggregation capacity due to T class mutants of RSV at 36 degrees C is dependent on constant maintenance of protein synthesis because addition of cycloheximide restored the aggregation capacity even at the permissive temperature.

Download Full-text

Association of microtubules and intermediate filaments in normal fibroblasts and its disruption upon transformation by a temperature-sensitive mutant of Rous sarcoma virus.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.78.11.6986 ◽

1981 ◽

Vol 78 (11) ◽

pp. 6986-6990 ◽

Cited By ~ 45

Author(s):

E. H. Ball ◽

S. J. Singer

Keyword(s):

Intermediate Filaments ◽

Rous Sarcoma Virus ◽

Temperature Sensitive ◽

Sensitive Mutant ◽

Temperature Sensitive Mutant ◽

Rous Sarcoma ◽

Sarcoma Virus

Download Full-text

Expression of the v-src or v-fps oncogene increases fructose 2,6-bisphosphate in chick-embryo fibroblasts. Novel mechanism for the stimulation of glycolysis by retroviruses

Biochemical Journal ◽

10.1042/bj2360595 ◽

1986 ◽

Vol 236 (2) ◽

pp. 595-599 ◽

Cited By ~ 34

Author(s):

L Bosca ◽

M Mojena ◽

J Ghysdael ◽

G G Rousseau ◽

L Hue

Keyword(s):

Chick Embryo ◽

Rous Sarcoma Virus ◽

Temperature Sensitive ◽

Permissive Temperature ◽

Kinase Activation ◽

Rous Sarcoma ◽

Sarcoma Virus ◽

Embryo Fibroblasts ◽

Rate Limiting ◽

Stimulation Of

The concentration of fructose 2,6-bisphosphate and the activity of 6-phosphofructo-2-kinase are increased after infection of chick-embryo fibroblasts with the Rous sarcoma virus, or with a temperature-sensitive mutant of this virus at the permissive, but not at the non-permissive, temperature. This is observed after transformation by retroviruses carrying either the v-src or v-fps, but not the v-mil and/or v-myc, oncogenes. Comparison of the effects of the Rous sarcoma virus with those of phorbol myristate acetate on fructose 2,6-bisphosphate suggests that both result from the stimulation of a step which is rate-limiting for 6-phosphofructo-2-kinase activation and which is also controlled by protein kinase C.

Download Full-text