Lack of correlation between membrane lipid composition and thermotolerance in Chinese hamster ovary cells

1982 ◽  
Vol 692 (1) ◽  
pp. 168-170 ◽  
Author(s):  
Ricardo Gonzalez-Mendez ◽  
Kenneth W. Minton ◽  
George M. Hahn
Keyword(s):  
Lipid Composition ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Membrane Lipid ◽  
Ovary Cells ◽  
Membrane Lipid Composition

Freeze fracturing properties of native and heat-adapted Chinese hamster ovary (CHO) cells

1989 ◽  
Vol 67 (8) ◽  
pp. 473-476
Author(s):  
Maria Dinda ◽  
Carol Quirt ◽  
William Mackillop ◽  
Michael Singer
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Freeze Fracture ◽  
Chinese Hamster ◽  
Membrane Lipid ◽  
Fracture Plane ◽  
Ovary Cells ◽  
Freeze Fracturing ◽  
Different Temperatures ◽  
History Of

Chinese hamster ovary cells were either maintained at 37 °C (native cells) or heat adapted by exposure to a temperature of 40 °C for 2 h. Thereafter, native and heat-adapted cells were incubated at different temperatures for various times, harvested, fixed with glutaraldehyde and glycerol, and studied by freeze fracture microscopy. We observed that the fracture plane either passed through the cell, exposing cytoplasm, or stayed within the plasma membrane, and that the location of the fracture plane was strongly determined by the previous thermal history of the cell. We believe that these differences reflect changes in membrane lipid packing arrangements.Key words: freeze fracture, heat adaptation, Chinese hamster ovary cells.

Malignancy-related characteristics of wild type and drug-resistant chinese hamster ovary cells

Pathology ◽  
1993 ◽  
Vol 25 (3) ◽  
pp. 268-276 ◽  
Author(s):  
Wanda B. Mackinnon ◽  
Marlen Dyne ◽  
Rebecca Hancock ◽  
Carolyn E. Mountford ◽  
Adrienne J. Grant ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Drug Resistant ◽  
Wild Type ◽  
Ovary Cells

Review of the current methods of Chinese Hamster Ovary (CHO) cells cultivation for production of therapeutic protein

2020 ◽  
Vol 17 ◽  
Author(s):  
Shazid Md. Sharker ◽  
Md. Atiqur Rahman
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Mass Production ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Therapeutic Protein ◽  
Specific Productivity ◽  
Ovary Cells ◽  
Further Development ◽  
Interesting Area

Most of clinical approved protein-based drugs or under in clinical trial have a profound impact in the treatment of critical diseases. The mammalian eukaryotic cells culture approaches, particularly the CHO (Chinese Hamster Ovary) cells are mainly used in the biopharmaceutical industry for the mass-production of therapeutic protein. Recent advances in CHO cell bioprocessing to yield recombinant proteins and monoclonal antibodies have enabled the expression of quality protein. The developments of cell lines are possible to upgrade specific productivity. As a result, it holds an interesting area for academic as well as industrial researchers around the world. This review will concentrate on the recent progress of the mammalian CHO cells culture technology and the future scope of further development for the mass-production of protein therapeutics.

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