Enzymatic synthesis in biphasic aqueous-organic systems. II. Shift of ionic equilibria

1981 ◽  
Vol 658 (1) ◽  
pp. 90-101 ◽  
Author(s):  
Karel Martinek ◽  
Anatole N. Semenov
Synlett ◽  
1991 ◽  
Vol 1991 (04) ◽  
pp. 310-312
Author(s):  
Patrizia Ferraboschi ◽  
Daria Brembilla ◽  
Paride Grisenti ◽  
Enzo Santaniello

2018 ◽  
Author(s):  
Andrea Pérez-Villa ◽  
Thomas Georgelin ◽  
Jean-François Lambert ◽  
Marie-Christine Maurel ◽  
François Guyot ◽  
...  

Understanding the mechanism of spontaneous formation of ribonucleotides under realistic prebiotic conditions is a key open issue of origins-of-life research. In cells, <i>de novo</i> and salvage nucleotide enzymatic synthesis combines 5-phospho-α -D-ribose-1-diphosphate ( α-PRPP) and nucleobases. Interestingly, these reactants are also known as prebiotically plausible compounds. Combining ab initio simulations with mass spectrometry experiments, we compellingly demonstrate that nucleobases and α -PRPP spontaneously combine, through the same facile mechanism, forming both purine and pyrimidine ribonucleotides, under mild hydrothermal conditions. Surprisingly, this mechanism is very similar to the biological one, and yields ribonucleotides with the same anomeric carbon chirality as in biological systems. These results suggest that natural selection might have optimized – through enzymes – a pre-existing ribonucleotide formation mechanism, carrying it forward to modern life forms.


2017 ◽  
Author(s):  
Andrea Pérez-Villa ◽  
Thomas Georgelin ◽  
Jean-François Lambert ◽  
Marie-Christine Maurel ◽  
François Guyot ◽  
...  

Understanding the mechanism of spontaneous formation of ribonucleotides under realistic prebiotic conditions is a key open issue of origins-of-life research. In cells, <i>de novo</i> and salvage nucleotide enzymatic synthesis combines 5-phospho-α -D-ribose-1-diphosphate ( α-PRPP) and nucleobases. Interestingly, these reactants are also known as prebiotically plausible compounds. Combining ab initio simulations with mass spectrometry experiments, we compellingly demonstrate that nucleobases and α -PRPP spontaneously combine, through the same facile mechanism, forming both purine and pyrimidine ribonucleotides, under mild hydrothermal conditions. Surprisingly, this mechanism is very similar to the biological one, and yields ribonucleotides with the same anomeric carbon chirality as in biological systems. These results suggest that natural selection might have optimized – through enzymes – a pre-existing ribonucleotide formation mechanism, carrying it forward to modern life forms.


10.28945/3391 ◽  
2009 ◽  
Author(s):  
Moshe Pelleh

In our world, where most systems become embedded systems, the approach of designing embedded systems is still frequently similar to the approach of designing organic systems (or not embedded systems). An organic system, like a personal computer or a work station, must be able to run any task submitted to it at any time (with certain constrains depending on the machine). Consequently, it must have a sophisticated general purpose Operating System (OS) to schedule, dispatch, maintain and monitor the tasks and assist them in special cases (particularly communication and synchronization between them and with external devices). These OSs require an overhead on the memory, on the cache and on the run time. Moreover, generally they are task oriented rather than machine oriented; therefore the processor's throughput is penalized. On the other hand, an embedded system, like an Anti-lock Braking System (ABS), executes always the same software application. Frequently it is a small or medium size system, or made up of several such systems. Many small or medium size embedded systems, with limited number of tasks, can be scheduled by our proposed hardware architecture, based on the Motorola 500MHz MPC7410 processor, enhancing its throughput and avoiding the software OS overhead, complexity, maintenance and price. Encouraged by our experimental results, we shall develop a compiler to assist our method. In the meantime we will present here our proposal and the experimental results.


2014 ◽  
Vol 32 (8) ◽  
pp. 1405-1410
Author(s):  
Lisheng XU ◽  
Junzhong LIU ◽  
Zhiyuan WANG ◽  
Hongjuan ZHANG ◽  
Wei LIU ◽  
...  

2008 ◽  
Vol 59 (11) ◽  
Author(s):  
Iulia Lupan ◽  
Sergiu Chira ◽  
Maria Chiriac ◽  
Nicolae Palibroda ◽  
Octavian Popescu

Amino acids are obtained by bacterial fermentation, extraction from natural protein or enzymatic synthesis from specific substrates. With the introduction of recombinant DNA technology, it has become possible to apply more rational approaches to enzymatic synthesis of amino acids. Aspartase (L-aspartate ammonia-lyase) catalyzes the reversible deamination of L-aspartic acid to yield fumaric acid and ammonia. It is one of the most important industrial enzymes used to produce L-aspartic acid on a large scale. Here we described a novel method for [15N] L-aspartic synthesis from fumarate and ammonia (15NH4Cl) using a recombinant aspartase.


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