Age-related changes in mRNA, protein and catalytic activity of hepatic neutral cholesterol ester hydrolase in male rats: evidence for transcriptional regulation

1996 ◽  
Vol 1302 (2) ◽  
pp. 153-158 ◽  
Author(s):  
Ramesh Natarajan ◽  
Shobha Ghosh ◽  
W. McLean Grogan
Keyword(s):  
Transcriptional Regulation ◽  
Catalytic Activity ◽  
Cholesterol Ester ◽  
Male Rats ◽  
Cholesterol Ester Hydrolase ◽  
Ester Hydrolase ◽  
Age Related ◽  
Age Related Changes

CHOLESTEROL ESTER HYDROLYSIS BY HOMOGENATES OF WHOLE TESTIS, SEMINIFEROUS TUBULES AND INTERSTITIAL CELLS OF MATURE RATS

1977 ◽  
Vol 75 (2) ◽  
pp. 235-243 ◽  
Author(s):  
J. P. RENSTON ◽  
T. J. IHRIG ◽  
R. H. RENSTON ◽  
B. GONDOS ◽  
R. J. MORIN
Keyword(s):  
Cholesterol Ester ◽  
Incubation Temperature ◽  
Hydrolytic Activity ◽  
Interstitial Cells ◽  
Seminiferous Tubules ◽  
Cholesterol Ester Hydrolase ◽  
Ester Hydrolase ◽  
Optimum Ph ◽  
Hormone Biosynthesis ◽  
Hydrolysis Of

The characteristics and localization of a cholesterol ester hydrolase enzyme in homogenates of whole testis and in isolated seminiferous tubules and interstitial cells of mature rats have been investigated. Hydrolysis of cholesteryl [1-14C]oleate occurred at an optimum pH of 7·0 was linearly related to time up to 5–6 h of incubation and increased linearly up to 0·25 mg protein/incubation. Hydrolytic activity was inhibited by increasing the incubation temperature from 29 to 41 °C and by sonication. Cholesterol ester hydrolase activity/mg protein was three times greater in homogenates of seminiferous tubules than in interstitial cells. Cholesterol ester hydrolase may function to provide precursors for use in seminiferous tubular steroid hormone biosynthesis or germ cell maturation.

scholarly journals Regulation of cholesterol ester hydrolase by cyclic AMP-dependent protein kinase

FEBS Letters ◽  
1986 ◽  
Vol 201 (2) ◽  
pp. 257-261 ◽  
Author(s):  
Roger J. Colbran ◽  
Andrew J. Garton ◽  
Susan R. Cordle ◽  
Stephen J. Yeaman
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Cholesterol Ester ◽  
Dependent Protein Kinase ◽  
Cholesterol Ester Hydrolase ◽  
Ester Hydrolase ◽  
Dependent Protein
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