In vitro HeLa cell DNA synthesis II. Partial characterization of soluble factors stimulating nuclear DNA synthesis

1978 ◽  
Vol 520 (2) ◽  
pp. 271-284 ◽  
Author(s):  
Janis M.K. Fraser ◽  
Joel A. Huberman
Keyword(s):  
Hela Cell ◽  
Dna Synthesis ◽  
Nuclear Dna ◽  
Partial Characterization ◽  
Soluble Factors

scholarly journals In vitro conversion of proapoprotein A-I to apoprotein A-I. Partial characterization of an extracellular enzyme activity.

1983 ◽  
Vol 258 (19) ◽  
pp. 11430-11433 ◽  
Author(s):  
C Edelstein ◽  
J I Gordon ◽  
K Toscas ◽  
H F Sims ◽  
A W Strauss ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Extracellular Enzyme ◽  
Extracellular Enzyme Activity ◽  
Partial Characterization

scholarly journals Involvement of deoxyribonucleic acid polymerase β in nuclear deoxyribonucleic acid synthesis

1978 ◽  
Vol 173 (1) ◽  
pp. 309-314 ◽  
Author(s):  
T R Butt ◽  
W M Wood ◽  
E L McKay ◽  
R L P Adams
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Deoxyribonucleic Acid ◽  
Nuclear Dna ◽  
Dna Polymerase Beta ◽  
Cell Nuclei ◽  
High Molecular Weight Dna ◽  
Dna Polymerase Alpha ◽  
Polymerase Beta

The effects on DNA synthesis in vitro in mouse L929-cell nuclei of differential extraction of DNA polymerases alpha and beta were studied. Removal of all measurable DNA polymerase alpha and 20% of DNA polymerase beta leads to a 40% fall in the replicative DNA synthesis. Removal of 70% of DNA polymerase beta inhibits replicative synthesis by 80%. In all cases the nuclear DNA synthesis is sensitive to N-ethylmaleimide and aCTP (arabinosylcytosine triphosphate), though less so than DNA polymerase alpha. Addition of deoxyribonuclease I to the nuclear incubation leads to synthesis of high-molecular-weight DNA in a repair reaction. This occurs equally in nuclei from non-growing or S-phase cells. The former nuclei lack DNA polymerase alpha and the reaction reflects the sensitivity of DNA polymerase beta to inhibiton by N-ethylmaleimide and aCTP.

scholarly journals Isolation and partial characterization of a pair of prolactins released in vitro by the pituitary of a cichlid fish, Oreochromis mossambicus.

1985 ◽  
Vol 82 (22) ◽  
pp. 7490-7494 ◽  
Author(s):  
J. L. Specker ◽  
D. S. King ◽  
R. S. Nishioka ◽  
K. Shirahata ◽  
K. Yamaguchi ◽  
...  
Keyword(s):  
Cichlid Fish ◽  
Oreochromis Mossambicus ◽  
Partial Characterization

Isolation and partial characterization of myogenic cells from mussel larvae in vitro

2000 ◽  
Vol 32 (5) ◽  
pp. 417-424 ◽  
Author(s):  
N.A. Odintsova ◽  
S.V. Plotnikov ◽  
A.A. Karpenko
Keyword(s):  
Partial Characterization ◽  
Myogenic Cells
Sign in / Sign up

Export Citation Format

Share Document