Isolation and identification of hemin as an endogenous Na+K+-ATPase inhibitor from porcine blood cells

1991 ◽  
Vol 178 (1) ◽  
pp. 95-103 ◽  
Author(s):  
Tadashi Yasuhara ◽  
Masaaki Mori ◽  
Kaori Wakamatsu ◽  
Kazuki Kubo
Keyword(s):  
Blood Cells ◽  
Atpase Inhibitor ◽  
Isolation And Identification ◽  
Porcine Blood

THE USE OF INHIBITORY MEDIA TO SCREEN SOUTH DAKOTA BULK TANK MILK FOR STREPTOCOCCUS AGALACTIAE

1972 ◽  
Vol 35 (11) ◽  
pp. 629-631 ◽  
Author(s):  
C. A. Kirkbride ◽  
Hazel Shave ◽  
James Blades
Keyword(s):  
South Dakota ◽  
Red Blood Cells ◽  
Streptococcus Agalactiae ◽  
Blood Cells ◽  
Ferric Citrate ◽  
Isolation And Identification ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank ◽  
Beta Toxin

Edward's medium with red blood cells, staphylococcus beta toxin, and ferric citrate (TKT medium) was used to culture 4189 bulk tank milk samples from herds supplying seven dairy plants in South Dakota. Identity of organisms with characteristics of Streptococcus agalactiae on TKT was tested by biochemical means. The incidence of S. agalactiae infected herds supplying the seven milk plants ranged from 0% to 66.6% and averaged 4.03%. Difficulty was encountered in identifying S. agalactiae by its characteristics on TKT alone so that only about one out of ten colonies picked as S. agalactiae from TKT had all the characteristics of this organism. Some limitation of the method for use in S. agalactiae eradication is thus indicated. Modified KF Agar was found unsuitable for the direct isolation and identification of S. agalactiae from bulk tank milk.

Heparin and EDTA as anticoagulant differentially affect cytokine mRNA level of cultured porcine blood cells

2007 ◽  
Vol 324 (1-2) ◽  
pp. 38-47 ◽  
Author(s):  
J.C. Duvigneau ◽  
W. Sipos ◽  
R.T. Hartl ◽  
M. Bayer ◽  
R. Moldzio ◽  
...  
Keyword(s):  
Blood Cells ◽  
Mrna Level ◽  
Cytokine Mrna ◽  
Porcine Blood ◽  
Cytokine Mrna Level

Development of livestock feed additives using porcine blood cells

2021 ◽  
Author(s):  
Jae‐Hwan Choi ◽  
Tae‐Hwan Jung ◽  
Hyeon‐Ju Seo ◽  
Kyoung‐Sik Han
Keyword(s):  
Blood Cells ◽  
Feed Additives ◽  
Livestock Feed ◽  
Porcine Blood

Preparation of Glycosylated Nitrosohemoglobin by Maillard Reaction and its Stability under Fluorescent Light at 20°C

2012 ◽  
Vol 550-553 ◽  
pp. 1094-1098
Author(s):  
Pei Jun Li ◽  
Bao Hua Kong ◽  
Hong Tao Zhang ◽  
Ning Li ◽  
Dong Mei Zheng ◽  
...  
Keyword(s):  
Maillard Reaction ◽  
Blood Cells ◽  
Fluorescent Light ◽  
Light Radiation ◽  
Optimum Ratio ◽  
Preparation Conditions ◽  
Porcine Blood ◽  
Pink Color ◽  
The Stability ◽  
Nitrosyl Hemoglobin

One of the roles nitrite played in meat curing is to produce the characteristic pink color, but the use is limited due to its potential carcinogenicity. In this study, porcine blood was used for synthesizing glycosylated nitrosyl-hemoglobin (G-NO-Hb) through maillard reaction and the preparation conditions were optimized. Also, the stability of G-NO-Hb was assessed by UV-Vis spectra. The results showed that: the optimum ratio of blood cells to water was 1: 2, nitrite of 2 mg/kg reaction mixture was chosen. The synthesized G-NO-Hb solution was rather stable under light radiation at 20°C for 20d. It revealed that G-NO-Hb was a potential stable pigment used for meat curing as a nitrite substitute.

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

Ultrastructural changes in murine lymphoma cells exposed to ultraviolet-A radiation

1991 ◽  
Vol 49 ◽  
pp. 104-105
Author(s):  
Delma P. Thomas ◽  
Dianne E. Godar
Keyword(s):  
Medical Devices ◽  
Blood Cells ◽  
White Blood Cells ◽  
Consumer Products ◽  
Ultrastructural Changes ◽  
Ultraviolet A ◽  
Uv Spectrum ◽  
Lymphoma Cells ◽  
Murine Lymphoma ◽  
Transmission Electron

Ultraviolet radiation (UVR) from all three waveband regions of the UV spectrum, UVA (320-400 nm), UVB (290-320 nm), and UVC (200-290 nm), can be emitted by some medical devices and consumer products. Sunlamps can expose the blood to a considerable amount of UVR, particularly UVA and/or UVB. The percent transmission of each waveband through the epidermis to the dermis, which contains blood, increases in the order of increasing wavelength: UVC (10%) < UVB (20%) < UVA (30%). To investigate the effects of UVR on white blood cells, we chose transmission electron microscopy to examine the ultrastructure changes in L5178Y-R murine lymphoma cells.

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

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