Lipid peroxidation as the cause of the ascorbic acid induced decrease of adenosine triphosphatase activities of rat brain microsomes and its inhibition by biogenic amines and psychotropic drugs

1. The adenosine-triphosphatase activity of rat-brain microsomes was measured between 0 degrees and 37 degrees . The stimulatory effect of Na(+) plus K(+) on the Mg(2+)-dependent adenosine-triphosphatase activity decreased sharply with decreasing temperature and became negligible at 0 degrees . An Arrhenius plot drawn from the experimental data showed two discontinuities: one at about 6 degrees and the other at about 20 degrees . 2. The increment in activity induced by Na(+) plus K(+) was more sensitive to oligomycin at lower than at higher temperatures, but the opposite was observed for ouabain. The action of oligomycin showed a biphasic character, since below a certain concentration it caused slight activation of Na(+)-plus-K(+)-activated adenosine triphosphatase. 3. Where oligomycin increased the activity of the enzyme, it also enhanced the accumulation of an acid-precipitable phosphorylated compound formed through the transfer of the gamma-phosphate group of [(32)P]ATP to the enzyme system. Stimulatory concentrations of oligomycin did not interfere with K(+)-mediated dephosphorylation of the intermediate, though high concentrations of oligomycin counteracted the effect of K(+). 4. The temperature profile of K(+)-stimulated microsomal phosphatase qualitatively resembled that of microsomal adenosine triphosphatase.

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RELATIONSHIP BETWEEN LIPID PEROXIDATION AND ENDOGENOUS PROSTAGLANDIN FORMATION IN RAT BRAIN HOMOGENATES: THE ROLE OF ASCORBIC ACID

Abstracts ◽

10.1016/b978-0-08-023768-8.51808-9 ◽

1978 ◽

pp. 689

Author(s):

A. Seregi ◽

M. Komlós ◽

A. Schaefer

Keyword(s):

Lipid Peroxidation ◽

Ascorbic Acid ◽

Rat Brain ◽

Endogenous Prostaglandin

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Inhibition by ascorbic acid of the sodium-plus-potassium ion-stimulated adenosine triphosphatase in rat brain

Biochemical Journal ◽

10.1042/bj1280139pa ◽

1972 ◽

Vol 128 (4) ◽

pp. 139P-139P ◽

Cited By ~ 1

Author(s):

R P Boxall ◽

P J Phizackerley

Keyword(s):

Ascorbic Acid ◽

Rat Brain ◽

Adenosine Triphosphatase ◽

Potassium Ion

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Effects of biogenic amines and psychotropic drugs on endogenous prostaglandin biosynthesis in the rat brain homogenates

Biochemical Pharmacology ◽

10.1016/0006-2952(78)90303-9 ◽

1978 ◽

Vol 27 (2) ◽

pp. 213-218 ◽

Cited By ~ 35

Author(s):

András Schaefer ◽

Martá Komlós ◽

András Seregi

Keyword(s):

Rat Brain ◽

Biogenic Amines ◽

Psychotropic Drugs ◽

Prostaglandin Biosynthesis ◽

Endogenous Prostaglandin

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Effect of selenium on vanadium toxicity in different regions of rat brain

Human & Experimental Toxicology ◽

10.1177/096032719801700104 ◽

1998 ◽

Vol 17 (1) ◽

pp. 23-28 ◽

Cited By ~ 8

Author(s):

Syed Saleem Haider ◽

A A Abdel-Gayoum ◽

Mustafa El-Fakhri ◽

Kilani M Ghwarsha

Keyword(s):

Lipid Peroxidation ◽

Ascorbic Acid ◽

Protective Effect ◽

Rat Brain ◽

Sulfhydryl Group ◽

Brain Regions ◽

Neurotoxic Effects ◽

Selenium Treatment ◽

Vanadium Toxicity ◽

The Brain

The protective effect of selenium on the neurotoxicity of vanadium in different brain regions of rats was investigated. The lipid peroxidation was significantly accentuated after intraperitoneal (i.p.) administration of vanadium (1.5 mg kg71 b.wt) for a period of 12 consecutive days to rats. The increase in lipid peroxidation was inhibited by selenium treatment (0.02 mg kg71 b.wt., i.p.) for 12 consecutive days. Vanadium exposure produced a decrease in nonprotein sulfhydryl group. Selenium treatment prevented the depression in nonprotein sulfhydryl group in all the brain regions of the vanadium exposed rats. The concentration of ascorbic acid was decreased after co-administration of selenium and vanadium. These results suggest that selenium protects neuronal cells against neurotoxic effects of vanadium by maintaining the availability of antioxidant nonprotein sulfhydryl groups. The decrease in ascorbic acid levels may have been due to its consumption in forming complexes with vanadium.

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