Activation of tyrosine hydroxylase in the central nervous system by anaerobiosis

1985 ◽  
Vol 34 (16) ◽  
pp. 2975-2982 ◽  
Author(s):  
Anna Pastuszko ◽  
David F. Wilson ◽  
Maria EreciŃska
Author(s):  
Klockner Géssica De Mattos Diosti ◽  
Lovato Fernanda Christo ◽  
Rebouças Rebeca Loureiro ◽  
Langer Laura Ingrid Volkweis ◽  
Hoegen Ingrid Oliveira ◽  
...  

1983 ◽  
Vol 31 (1) ◽  
pp. 62-68 ◽  
Author(s):  
J C King ◽  
R M Lechan ◽  
G Kugel ◽  
E L Anthony

Acrolein was examined as an alternative fixative to formaldehyde for immunocytochemical localization of neuropeptides in the rat brain. A brief (5 min) vascular perfusion with a 5% acrolein solution allowed the identification of thyrotropin-releasing hormone (TRH), vasoactive intestinal peptide (VIP), somatostatin (SRIF), neurotensin (NT), methionine enkephalin (Menk), adrenocorticotropic hormone (ACTH), tyrosine hydroxylase (TH), and luteinizing hormone-releasing hormone (LHRH) in fibers and perikarya within the central nervous system of the rat using the peroxidase-antiperoxidase (PAP) technique. Acrolein appears to be particularly valuable for immunocytochemistry, as it 1) stabilizes heterogeneous peptides and proteins rapidly and effectively, 2) retains antigenicity, and 3) preserves morphological detail.


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