Microglial cell reaction in the gray and white matter in spinal cords from jimpy mice. An enzyme histochemical study at the light and electron microscope level

1995 ◽  
Vol 694 (1-2) ◽  
pp. 287-298 ◽  
Author(s):  
JoséM. Vela ◽  
Ishar Dalmau ◽  
Laia Acarín ◽  
Berta González ◽  
Bernardo Castellano
Keyword(s):  
Electron Microscope ◽  
White Matter ◽  
Microglial Cell ◽  
Histochemical Study ◽  
Microscope Level ◽  
Cell Reaction ◽  
Electron Microscope Level ◽  
Jimpy Mice

Sequential Changes Associated with Cell Wall Formation and Fusion in the Vascular Cambium

IAWA Journal ◽  
1981 ◽  
Vol 2 (4) ◽  
pp. 151-162 ◽  
Author(s):  
A.M. Catesson ◽  
J.C. Roland
Keyword(s):  
Cell Wall ◽  
Electron Microscope ◽  
Cell Walls ◽  
Microscope Level ◽  
Cell Wall Formation ◽  
Characteristic Sequence ◽  
Electron Microscope Level ◽  
Starting Point ◽  
Cell Wall Development ◽  
Cambial Zone

Cytochemical techniques and mild extractions were used at the electron microscope level for the study of the cambial zone of several hardwoods and one softwood. The maturation processes of the primary radial and tangential cell walls involve a progressive disappearance of their initial heterogeneity. The buttress-like zone joining these walls appears to be the starting point for a characteristic sequence of changes and intra-wall rearrangement. Topochemical results have suggested an alternative to the 'emboxing concept' of cell wall development.

scholarly journals CYTOCHEMISTRY OF 5-HYDROXYTRYPTAMINE AT THE ELECTRON MICROSCOPE LEVEL I. STUDY OF THE SPECIFICITY OF THE REACTION IN ISOLATED BLOOD PLATELETS

1968 ◽  
Vol 16 (3) ◽  
pp. 162-171 ◽  
Author(s):  
GUILLERMO JAIM ETCHEVERRY ◽  
LUIS MARIA ZIEHER
Keyword(s):  
Electron Microscope ◽  
Blood Platelets ◽  
Microscope Level ◽  
Storage Site ◽  
Electron Microscope Level ◽  
Dense Granules ◽  
Amine Content ◽  
Level I ◽  
Previous Assumption

Blood platelets obtained from normal rabbits and those isolated from reserpine-treated animals and subsequently incubated in vitro with 5-hydroxytryptamine, norepinephrine and histamine were assayed for amine content or processed for examination under the electron microscope. With the glutaraldehyde-dichromate reaction for unsubstituted catechol- and indoleamines, reactive granules were observed in normal platelets. Formaldehyde fixation prior to the glutaraldehyde-dichromate reaction resulted in a similar image under the electron microscope. In platelets obtained from animals treated with reserpine a decrease of the amine content with a corresponding reduction in the number of dense granules was observed. Following incubation with 5-hydroxytryptamine the concentration of the amine increased markedly and the number of dense granules that reacted with both techniques became practically normal. In norepinephrine-incubated platelets dense granules were demonstrated with the glutaraldehyde-dichromate reaction, but no reactive products were observed using prefixation with formaldehyde. Histamine was also incorporated into depleted platelets but gave no reaction. It is concluded that prefixation with formaldehyde renders negative the reaction with catecholamines, leaving unaffected indoleamine-reactive sites. The previous assumption that the dense granules contain 5-hydroxytryptamine has been confirmed by such a cytochemical approach. The possibility that these organelles constitute a common storage site for different amines is discussed.

Infundibulomyces: a new genus of coelomycetes from Thailand

2003 ◽  
Vol 81 (7) ◽  
pp. 732-737 ◽  
Author(s):  
Narumol Plaingam ◽  
Sayanh Somrithipol ◽  
E B. Gareth Jones
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscope ◽  
Type Species ◽  
New Genus ◽  
Anamorphic Fungi ◽  
Microscope Level ◽  
Electron Microscope Level ◽  
Fallen Leaves ◽  
Scanning Electron

The genus Infundibulomyces is proposed for coelomycetous fungi with nidulariaceous-like conidiomata and bipolar appendaged conidia. Infundibulomyces cupulata sp. nov., collected from fallen leaves of Lagerstroemia sp. from Thailand, is described as the type species of the genus. This fungus is illustrated at the light microscope and scanning electron microscope level and compared with other morphologically related taxa. Cupulate conidiomata are uncommon and Infundibulomyces is compared with these.Key words: anamorphic fungi, coelomycete, Infundibulomyces, Thailand, tropical.

scholarly journals alpha 2-macroglobulin adsorbed to colloidal gold: a new probe in the study of receptor-mediated endocytosis.

1981 ◽  
Vol 89 (1) ◽  
pp. 29-34 ◽  
Author(s):  
R B Dickson ◽  
M C Willingham ◽  
I Pastan
Keyword(s):  
Electron Microscope ◽  
Cell Surface ◽  
Vesicular Stomatitis Virus ◽  
Golgi Complex ◽  
Colloidal Gold ◽  
Microscope Level ◽  
Coated Pits ◽  
Electron Microscope Level ◽  
Receptor Mediated Endocytosis ◽  
Vesicular Stomatitis

alpha 2-Macroglobulin (alpha 2 M) was adsorbed to colloidal gold and used as a new tool in the study of receptor-mediated endocytosis. alpha 2 M-gold is easy to prepare and is clearly visualized at the electron microscope level. When cells were incubated with alpha 2 M-gold at 0 degrees C, gold was visualized both diffusely over the cell surface and concentrated in coated pits. After cells to which alpha 2 M-gold had been bound at 0 degrees C were warmed, the gold was rapidly internalized into uncoated vesicles, previously termed receptosomes. After 30 min of incubation or longer, gold was found in small lysosomes and, later, in large lysosomes and very small vesicles in the region of the Golgi complex. This pattern of localization is similar to that previously described, using peroxidase-labeled anti-alpha 2 M antibodies. By incubating cells with both alpha 2 M-gold and vesicular stomatitis virus (VSV), we studied the internalization of these two markers simultaneously. VSV and alpha 2 M-gold rapidly clustered in the same coated pits and were internalized in the same receptosomes. Proteins and hormones adsorbed to gold may be useful in the study of receptor-mediated endocytosis.

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