STUDY OF HELMINTH FAUNA OF THE EUROPEAN BISON IN THE KALUGA REGION

This article describes the study of the parasitic fauna of the European bison in the national Park "Ugra", the Kaluga Region. In the Russian Federation, this species has an official status of endangered species, therefore it is listed in the Red Book. In the Kaluga Region, there are several areas where the bison lives; in this work, the study area was the Dzerzhinsky District. The aim of the study was to detect and differentiate helminths, and provide therapeutic and preventive recommendations. The experiments were carried out using methods of flotation and sequential washings. Six species were selected as study helminths from three main classes of parasites: 1 species of helminths Dicrocoelium lanceatum from the class of trematodes (Trematoda). This parasite is localized in the liver and gall bladder. Three species of tapeworms from the class of cestodes (Cestoda): Moniezia expansa, which affects the small intestine, Cysticercus taenuicollis (Taenia hydatigena) and Cysticercus bovis (Taeniarhynchus saginatus), which can affect the musculature of animals. Two species of the parasite from the class of nematodes (Nematoda): Oesophagostomum radiatum and Nematodirus helvetianus, which are mainly localized in the small intestine. The topic of helminth fauna of the bison requires further study, since these animals can be the main sources of invasion. They live in the same biotopes as other ruminants, therefore they can cause infection of cattle and small ruminants that are in nearby territories for grazing.

Cysticercus bovis in cattle slaughtered in North Egypt: Overestimation by the visual inspection method

Background and Aim: The World Health Organization and the Food and Agriculture Organization list Taenia saginata, a foodborne cestode, as the most widely distributed human tapeworm worldwide. The larval stage of T. saginata, Cysticercus bovis, causes cysticercosis in bovines and infects humans who eat raw or undercooked beef. The existing detection methods of C. bovis in cattle depend on the visual inspection of meat. This study aimed to confirm the identification of C. bovis through visual inspection at the slaughterhouses in North Egypt with a molecular diagnosis. Materials and Methods: A total of 687 locally bred cattle (Baladi), including 428 cows and 259 buffaloes, slaughtered in four slaughterhouses in North Egypt from April 2018 to February 2019 were inspected for C. bovis using the traditional meat inspection method. Positive samples were verified through polymerase chain reaction (PCR) amplification and HDP2 gene sequencing. Results: Through visual inspection, C. bovis was detected in 4.2% and 12.4% of the slaughtered cows and buffaloes, respectively. Molecular analysis confirmed that 1.9% of the animals, all of which were cows, had C. bovis infection. DNA sequencing verified the identity of the PCR-amplified product. Conclusion: The rate of C. bovis infection in slaughterhouses detected through meat inspection is overestimated compared with that through PCR. Although meat inspection can be used as a primary screening tool for C. bovis, a more specific molecular method is required to achieve an accurate diagnosis.

Prevalence and geographical distribution of bovine cysticercosis in the mesoregion of Northern Minas Gerais

ABSTRACT The knowledge of the prevalence of bovine cysticercosis is important for the implementation of control programs. The objective was to evaluate the prevalence and geographical distribution of bovine cysticercosis in Northern Minas Gerais. For that, 14,556 cattle were evaluated during the federal post-mortem inspection in 27 municipalities of five microregions: Janaúba, Montes Claros, Januária, Pirapora and Bocaiúva. The prevalence of Cysticercus bovis was 1.03% with distribution in all microregions evaluated of the northern Minas Gerais. All municipalities with a sampling number of cattle slaughtered above 543 showed positive animals for cysticercosis. The microregions of Bocaiúva, Montes Claros, Janaúba, Januária and Pirapora presented a prevalence of cysticercosis at 2.11%, 1.17%, 1.01%, 0.90% and 0.56%, respectively. The microregions of Montes Claros and Januária presented a greater chance of finding positive cattle for cysticercosis. As there are positive cases of cysticercosis in all microregions evaluated, there is a risk to human health.

Antibody immunoglobulin G1 and immunoglobulin G2a responses against some cystic fluid proteins of Cysticercus bovis in Balb/c mice

Background and Aim: Immunoglobulin (Ig) G1 and IgG2a are the surrogate markers respectively for humoral and cellular immune responses of hosts against antigens including cystic fluid proteins of Cysticercus bovis. A study was conducted to investigate the IgG1 and IgG2a responses of Balb/c mice against some individual cystic fluid proteins of C. bovis in an effort to determine the roles of each protein in inducing the humoral and cellular immune responses in host. Materials and Methods: Individual p71, p31, and p14 proteins of C. bovis were purified by separation of the proteins using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and elution of individual proteins from the gel. Six female Balb/c mice were immunized 4 times at 10-day intervals with the crude cystic fluid proteins, and sera were collected for the measurement of IgG1 and IgG2a levels against the individual proteins. Sera samples collected before the first immunization were used as negative antibody control, sera samples collected after the fourth immunization were used as positive antibody control, and crude cystic fluid protein was used as positive antigen control. Results: All immunized mice were immune to p71, p31, p14, and crude cystic fluid proteins of C. bovis. The crude cystic fluid proteins of C. bovis induced a higher IgG2a than IgG1 level following the first and the second immunizations but switched into a higher IgG1 than IgG2a level following the fourth immunization. Protein 71 kDa (p71) induced a higher IgG2a than IgG1 level following the fourth immunization. In contrast, p14 induced a higher IgG1 than IgG2a level following the fourth immunization. Low and balance IgG1 and IgG2a levels against p31 were observed following the first to the fourth immunizations. Conclusion: Using IgG1 and IgG2a levels as the surrogate markers, it appears that cystic fluid antigens of C. bovis induce both humoral and cellular immune responses in Balb/c mice. The p71 appears to be a better inducer of cellular immune response, whereas p14 is a better inducer of humoral immune response of mice.

Epizootology (epidemiology) of helminthozoonoses in the south-west of Republic of Kazakstan

The purpose of the research: to educe regional specificity of epizootology (epidemiology) of helminthozoonoses (taeniarinhosis - cysticercosis, echinococcosis, trichinellosis) in Kyzylorda region Republic of Kazakstan. Materials and methods. In 2010-2016 according to postmortem examination methods cattle is investigated on cysticercosis and echinococcosis, sheep camels and horses on echinococcosis, pigs on taeniasis saginata at the premises of Kyzylorda meat cutting plant and at Kyzylorda markets. Physiological state of cysticercuses and caseworm larvocyst have been studied depending on the animal age. Invasion extensiveness (IE) of dogs were studied by the method of control worming by hydrobromide arecoline. As well as veterinarian and medical audit in region for the last 10 years was analyzed. Results and discussion. It has been established that cysticercosis bovine is widespread endemic and irregular. Invasion extensiveness (IE) of cattle was higher on the south-east of region (3.3%), and on the west this measure greatly declined (2.4%). This zoonosis extensiveness is greatly progressed during last 7 years (from 1.7% in 2010 till 3.3% in 2016). In general, at the south-west part of Kazakstan cattle is infected by Cysticercus bovis on 2.6-2.9% while the invasion strength (IS) is average 2-4 cysticercuses onto one corpse. During 2010-2016 it was educed two persons who are ill on taeniasis saginata. Sheep, cattle and camels were average taken by caseworms on 21.1%, 8.1%, and 42.7% respectively. IE parameters depended on the age and were high at the adult animals: Lambs are wormy on 2.5%, sheep older then 5 years on 29.5%, young camels at the age under 2 years are wormy on 27.9%, and animals older then 7 years on 50.0%, calves under 2 years are wormy on 2.4%, and cattle older then 5 years on 10.7%. IS of all kinds of animals correlated outright to EI and also depended on the animal age. In the process of internal veterinarian sanitary examination of 1680 killing horses of different age caseworm larvocyst were not founded. According to the data from health statistics, 463 patients (including 47.7% of men and 52.6% of women) have been operated concerning caseworm in the clinics in Kyzylorda region during 12 years (2005-2016 years). In 2010-2016 it was tested 688 samples of muscles of imported pork for trichinellosis by compressor method. All samples were negative. Over the last 10 years caseworm of animal and human was not registered in the region except one outburst in 2016 (February-March), during which 20 accidents of human caseworm had been educed in the region. The source of invasion was wild boars meat.

Značajne vrste parazita preživača na području Travnika

Na osnovi izvršenih parazitoloških pretraga unutrašnjih organa i poprečno-prugaste muskulature 1.939 preživača (1.006 teladi, 182 junadi, 538 goveda i 213 ovaca) iz individualnog uzgoja, podrijetlom s područja općine Travnik, kod 39,65 % životinja utvrđeno je prisustvo parazitskih vrsta iz klase Trematoda i larvalnih oblika parazita klase Eucestoda. Utvrđene su četiri vrste parazita, od čega dvije vrste trematoda Fasciola hepatica i Dicrocoelium dendriticum i dvije vrste larvalnih oblika eucestoda, Echinococcus polymorphus (vrste Echinococcus granulosus) i Cysticercus bovis (vrste Taenia saginata). Najveći ukupni postotak infestacije pretraženih preživača od 29,55 % opažen je za ciste Echinococcus polymorphus s najčešćim nalazom u jetri i plućima, a sporadično su nađeni na srcu, slezeni i bubregu. Dvostruko manji nalaz utvrđen je za Dicrocoelium dendriticum, 15,57 %, zatim Fasciola hepatica 8,45 %, a najmanji je utvrđen za bobice Cysticercus bovis, samo 0,41 %.