Allocation of the suppressive activity of normal peripheral blood lymphocytes induced by diffusion chamber culture and con A stimulation to the G2 phase of the cell cycle

1985 ◽  
Vol 94 (1) ◽  
pp. 21-31 ◽  
Author(s):  
K. Pachmann ◽  
U. Pachmann ◽  
R. Penning
Keyword(s):  
Cell Cycle ◽  
Peripheral Blood ◽  
Diffusion Chamber ◽  
Peripheral Blood Lymphocytes ◽  
Suppressive Activity ◽  
Con A ◽  
Normal Peripheral Blood ◽  
Blood Lymphocytes ◽  
Diffusion Chamber Culture ◽  
G2 Phase

scholarly journals Bleomycin sensitivity in patients with familial and sporadic polyposis: a pilot study

1999 ◽  
Vol 22 (1) ◽  
pp. 17-20
Author(s):  
Magaly M. Sales ◽  
Edmundo J. de Lucca ◽  
Seizo Yamashita ◽  
Luis Henrique Cury Saad
Keyword(s):  
Pilot Study ◽  
Familial Adenomatous Polyposis ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Adenomatous Polyposis ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Normal Individuals ◽  
G2 Phase

Human peripheral blood lymphocytes from 10 patients with familial adenomatous polyposis (FAP) showed a significantly higher incidence of chromatid breaks when compared to cells from 10 normal individuals, after exposure to bleomycin (BLM) during the G2 phase. However, no significant increase in bleomycin sensitivity was observed in lymphocytes from 10 patients with sporadic adenomatous polyps (AP) vs. 10 normal individuals (P = 0.67). Individuals that exhibited an average number of chromatid breaks per cell higher than 0.80 were considered sensitive to the drug. No control showed susceptibility to BLM, as compared to 3 out of 20 patients.

Aluminum-induced micronuclei and apoptosis in human peripheral-blood lymphocytes treated during different phases of the cell cycle

2005 ◽  
Vol 20 (4) ◽  
pp. 402-406 ◽  
Author(s):  
Anna Banasik ◽  
Anna Lankoff ◽  
Agnieszka Piskulak ◽  
Katarzyna Adamowska ◽  
Halina Lisowska ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes

scholarly journals Sezary cell morphology induced in peripheral blood lymphocytes: re- evaluation

Blood ◽  
1989 ◽  
Vol 73 (6) ◽  
pp. 1603-1607
Author(s):  
AC Chu ◽  
JF Morris
Keyword(s):  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Lymphocytes ◽  
Epidermal Cells ◽  
Lymphoid Cells ◽  
T Helper ◽  
Normal Peripheral Blood ◽  
Blood Lymphocytes ◽  
Driven System ◽  
Sézary Cells

In this study we examined the effect of mitogens and epidermal cells in inducing a Sezary cell morphology in normal peripheral blood lymphocytes. Peripheral blood mononuclear cells from six healthy volunteers were stimulated with the mitogens phytohemaglutinin and concanavalin A, and also cocultivated with human epidermal cell cultures. Incubation times with mitogens and epidermal cells were four days and stimulation of the lymphocytes by mitogens was confirmed by standard 3H-thymidine uptake. Standard transmission electron microscopy showed that in the mitogen-driven system 20% to 60% (33 +/- 15%) and in the epidermal cell-driven system 5% to 15% (8 +/- 4%) of the lymphoid cells exhibited mild to moderate indentation of the nuclei with nuclear contour indices (NCI) of 4.6 to 6.5 but no Sezary cells were observed (cells with NCI greater than 6.5 and up to 19.2). In the mitogen- stimulated preparation 2% to 5% (3 +/- 1%) of the lymphoid cells showed nuclear multilobulation resembling the cells seen in adult T cell lymphoma/leukemia. Incubation of mononuclear cells for longer periods of up to 4 weeks with mitogens and exogenous IL-2 resulted in no further morphologic changes. Using an indirect immunogold technique at the electron microscopic level, the cells showing nuclear indentation or lobulation were shown to bear both T helper (CD4) and T suppressor (CD8) cell phenotypes in a similar ratio to the total numbers of T helper and T suppressor cells present. Mitogens and epidermal cells are thus not able to induce a morphologic change to Sezary cells in normal peripheral blood lymphocytes.

scholarly journals The Transfer RNA Methylases of Human Lymphocytes. I. Induction by PHA in Normal Lymphocytes

Blood ◽  
1971 ◽  
Vol 37 (3) ◽  
pp. 282-292 ◽  
Author(s):  
DAVID H. RIDDICK ◽  
ROBERT C. GALLO
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Peripheral Blood Lymphocytes ◽  
Transfer Rna ◽  
Normal Peripheral Blood ◽  
Blood Lymphocytes ◽  
Normal Human ◽  
Total Capacity ◽  
Normal Human Peripheral Blood

Abstract Assays were performed that measured both the rate and extent (or total capacity) of transfer RNA methylases in extracts of lymphocytes cultured in the presence and absence of phytohemagglutinin (PHA). The tRNA methylases of human peripheral blood lymphocytes undergoing blastogenesis in culture with PHA had a five- to sixfold increase in rate and a three- to sevenfold increase in extent of methylation of heterologous tRNA. These data suggest that PHA transformed lymphocytes not only contain elevated levels of tRNA methylases, but that the increase includes qualitatively different enzymes from those found in normal peripheral blood lymphocytes. Experiments in which lymphocytes were incubated for various times with PHA revealed that tRNA methylase induction occurred late in or after DNA synthesis and after morphologic transformation, but prior to mitosis. Rate and extent of tRNA methylation increased simultaneously. PHA induction of tRNA methylase activity was dependent on the synthesis of new RNA in lymphocytes cultured from 40 to 45 hours. The increase was not due to different levels of inhibitors or activators or preferential degradation of reaction components. The data suggest that quantitative and qualitative changes occur in the tRNA methylases of the normal human peripheral blood lymphocyte stimulated by PHA to undergo transition to an undifferentiated cell "in cycle." The possible significance of these findings to control of protein synthesis in PHA transformed lymphocytes is discussed.

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