Micro-determination of human plasma renin activity with the addition of homologous substrate

1968 ◽  
Vol 22 (3) ◽  
pp. 309-315 ◽  
Author(s):  
Kikuo Arawaka ◽  
Atsushi Minohara ◽  
Junko Yamada ◽  
Nobuhiro Uemura ◽  
Motoomi Nakamura
Keyword(s):  
Plasma Renin Activity ◽  
Human Plasma ◽  
Plasma Renin ◽  
Renin Activity ◽  
Homologous Substrate

A method for determination of renin activity in dog's plasma and renal tissue

1970 ◽  
Vol 48 (7) ◽  
pp. 457-462 ◽  
Author(s):  
P. Granger ◽  
R. Boucher ◽  
J. Genest
Keyword(s):  
Plasma Renin Activity ◽  
Plasma Renin ◽  
Renal Tissue ◽  
Renin Activity ◽  
Dog Plasma ◽  
Healthy Dogs ◽  
The Mean ◽  
Homologous Substrate

A procedure based on that of Boucher et al. (Can. J. Physiol. Pharmacol. 45, 881 (1967)) for the microdetermination of plasma renin activity in the rat is described for the measurement of the same parameter in 1 ml of dog plasma. This procedure permits serial determinations and uses homologous substrate with incubation with Dowex 50W-X2 (NH4+) at pH 5.5 for 12 h. The results are expressed as nanograms of angiotensin formed per milliliter of plasma after 12 h of incubation. The mean plasma renin activity in healthy dogs was 1.04 ng/ml per h, ± 1.03 S.D., with a range of 0–3.3 ng. This procedure was applied to the measurement of renin activity in renal tissue.

scholarly journals Estimation of the efficacy of the measurement of plasma renin activity and direct renin in diagnostics of primary aldosteronism

2012 ◽  
Vol 58 (5) ◽  
pp. 21-27
Author(s):  
N P Goncharov ◽  
G S Kolesnikova ◽  
G V Katsiia ◽  
E Iu Rogal'
Keyword(s):  
Plasma Renin Activity ◽  
Primary Aldosteronism ◽  
Plasma Renin ◽  
Pressure Control ◽  
Renin Activity ◽  
Differential Diagnostics ◽  
Control Groups ◽  
Aldosterone Level ◽  
Renin Level

The objective of the present study was to estimate the informative value of the measurements of aldosterone level, direct renin, and plasma renin activity as well as the relationships between these characteristics for differential diagnostics of various forms of hypertension and, first and foremost, of primary aldosteronism. We have examined a total of 162 patients. The results of differential tests were used to allocate them to a few groups including 41 patients presenting with primary aldosteronism, 52 ones with incidentalomas, 26 with essential hypertension, and 43 with various endocrine diseases and normal arterial pressure (control groups). The aldosterone levels, direct renin, and plasma renin activity were measured in blood samples taken in morning hours from the patients in the supine position. The aldosterone to plasma renin activity (A/PRA) and aldosterone to direct renin (A/DR) ratios were calculated. The elevated blood aldosterone level is currently believed to be the principal criterion for primary aldosteronism in the patients suffering arterial hypertension. The RIA technology is the method of choice for the measurement of aldosterone levels. The determination of the A/PR ratio significantly improves the detectability of the disease. The use of direct renin level instead of kinetic renin ensures the high efficacy of screening for primary aldosteronism and its early diagnostics. The cut-off point for the calculation of the A/PRA ratio to differentiate between primary aldosteronism and incidentalomas is 2160 pmol/mcg/hr (sensitivity 100%, specificity 97.8%) in comparison with the analogous cut-off point for the discrimination between primary aldosteronism and endocrine pathology without hypertension is 49 pmol/mU (sensitivity 100%, specificity 95%). The cut-off point for the calculation of the A/PR ratio to differentiate between primary aldosteronism and incidentalomas is 2160 pmol/mcg/hr (sensitivity 89.5%, specificity 99%) in comparison with the analogous cut-off point for the discrimination between primary aldosteronism and endocrine pathology without hypertension is 1432 pmol/mcg/hr (sensitivity 89.5%, specificity 100%). It is concluded that the results of determination of direct renin level in the blood plasma are independent of the endogenous angiotensinogen level, less variable and more reproducible than than the results of the measurement of plasma renin activity. The aldosterone to direct renin ratio may be used for the screening of primary aldosteronism.

Dissociation in the Excretion of Different Aldosterone Metabolites and Unmetabolized (‘Free’) Aldosterone in Hypertension

1979 ◽  
Vol 57 (5) ◽  
pp. 409-414 ◽  
Author(s):  
S. Abdelhamid ◽  
P. Vecsei ◽  
D. Haack ◽  
K.-H. Gless ◽  
D. Walb ◽  
...  
Keyword(s):  
Essential Hypertension ◽  
Plasma Renin Activity ◽  
Plasma Renin ◽  
Renin Activity ◽  
Primary Hyperaldosteronism ◽  
Morphological Abnormality

1. The determination of aldosterone-18-glucuronide (pH 1-labile aldosterone) was complemented by concomitant measurements of free urinary aldosterone and tetrahydroaldosterone in 307 patients, most of whom were hypertensive. In 38 cases (12·3%) the normal, aldosterone-18-glucuronide concentration was clinically misleading, but increased free aldosterone and/or tetrahydroaldosterone values suggested the presence of hyperaldosteronism, which in many of these cases was confirmed by elevated excretion of the possible major aldosterone precursor 18-hydroxycorticosterone (18-OH-B). 2. Of 224 patients with essential hypertension and normal or low plasma renin activity 18 had an elevated free aldosterone and/or tetrahydroaldosterone excretion without increased aldosterone-18-glucuronide. These cases may represent early or pre-symptomatic forms of primary hyperaldosteronism. In other cases, particularly when tetrahydroaldosterone was increased alone, abnormalities of aldosterone metabolism were suspected. 3. In two out of 15 patients with primary hyperaldosteronism, aldosterone-18-glucuronide values were frequently found to be normal, although elevations were noted in other variables. However, no relation to the morphological abnormality (adenoma versus hyperplasia) was seen.

Determination of Plasma Renin Activity by Radioimmunoassay of Angiotensin I

1973 ◽  
Vol 44 (1) ◽  
pp. 43-54 ◽  
Author(s):  
S. Fukuchi ◽  
T. Takeuchi ◽  
T. Torikai
Keyword(s):  
Plasma Renin Activity ◽  
Simple Procedure ◽  
Plasma Renin ◽  
Inhibitory Effect ◽  
Renin Activity ◽  
Rabbit Serum ◽  
Chronic Glomerulonephritis ◽  
Angiotensin I ◽  
Standard Curve

1. A simple, rapid radioimmunoassay of angiotensin I has been applied to the measurement of plasma renin activity. 2. Antibody to angiotensin I was raised in rabbits by injecting angiotensin I conjugated with rabbit serum albumin. 3. Angiotensin I was generated in plasma by 3 h incubation at 37°C and pH 5.5 after adding EDTA and di-isopropylfluorophosphate (DFP). 4. The simple procedure of boiling for 10 min was performed to eliminate the inhibitory effect of plasma protein on immunoassay. After centrifugation, the supernatant was incubated for 18 h with 131I-labelled angiotensin I and antiserum. Free fractions of 131I-labelled angiotensin I were separated using dextran-coated charcoal, and compared with the standard curve. 5. Mean recovery of renin through the method was 91.8%; mean recovery of angiotensin I was 87.0%. 6. Normal values for plasma renin activity (estimated as the rate of generation of angiotensin I) was 1.17±0.90 ng ml−1 h−1; n = 21. Plasma renin activity was normal in essential hypertension; high in chronic glomerulonephritis with oedema; often high in renovascular hypertension; and low in primary aldosteronism.

Micro determination of plasma renin activity in normal infants and children on capillary and venous blood

1979 ◽  
Vol 99 (1) ◽  
pp. 93-95 ◽  
Author(s):  
Michéle Dechaux ◽  
Jean-Marie Limal ◽  
Michel Broyer ◽  
Charles Sachs
Keyword(s):  
Plasma Renin Activity ◽  
Venous Blood ◽  
Plasma Renin ◽  
Infants And Children ◽  
Renin Activity

Factor XII and Prekallikrein—Kallikrein—Kinin in the Cryoactivation of Human Plasma Prorenin

1984 ◽  
Vol 66 (5) ◽  
pp. 533-539 ◽  
Author(s):  
C. F. Brown ◽  
D. H. Osmond
Keyword(s):  
Plasma Renin Activity ◽  
Human Plasma ◽  
Plasma Renin ◽  
Determine Factor ◽  
Renin Activity ◽  
Factor Xii ◽  
Dextran Sulphate ◽  
Rat Uterus

1. Cryoactivation of human plasma ‘prorenin’ was followed for 24 h at −4°C. Chromogenic assays were used to determine factor XII (FXII), FXIIa, prekallikrein and kallikrein in relation to the observed cold-induced increase in plasma renin activity (PRA). Bradykinin activity was also determined using the rat uterus bioassay. 2. PRA increased rapidly and became significantly higher after just 6 h of cryoactivation, by which time prekallikrein had almost disappeared, while kallikrein and kinin levels increased. In contrast, FXII did not change notably, but some FXIIa was indeed formed. 3. The bacteriostat neomycin sulphate did not affect the course of cryoactivation, but did block the dextran sulphate- and kaolin-induced activation of prekallikrein and FXII respectively, and was therefore omitted. 4. Thus cryoactivation of prorenin is accompanied by, and may depend upon, the activation of FXII and prekallikrein, supporting other evidence in favour of this hypothesis.

Radioimmunoassay of plasma renin activity.

1976 ◽  
Vol 22 (2) ◽  
pp. 250-256 ◽  
Author(s):  
F Fyhrquist ◽  
P Soveri ◽  
L Puutula ◽  
U H Stenman
Keyword(s):  
Plasma Renin Activity ◽  
Enzyme Inhibitor ◽  
Proteolytic Enzymes ◽  
Plasma Renin ◽  
Renin Activity ◽  
Human Renin ◽  
Activity Measurements ◽  
Single Addition ◽  
Radioimmunoassay Method

Abstract We describe a sensitive, simplified radioimmunoassay method for determination of plasma renin activity. Plasma was acidified to the optimal pH (6.0) of angiotensin l generation with the least possible dilution, by using a single addition of hydrochloric acid and the enzyme inhibitor hydroxyquinoline. Recovery of unlabeled angiotensin l added to plasma was 92-97%; that of monoiodinated angiotensin l exceeded 90%, indicating satisfactory protection from proteolytic enzymes. Plasma constituents interfered little with the radioimmunoassay. Bland values for plasma kept at 0 degrees C were 10.7 +/- 2.3 (mean +/- SD) percent of the activity values for samples kept at +37 degrees C (n equals 63). In the routine setting, 6.25 pg of angiotensin l or 10(-6) Goldblatt units of Standard Human Renin was detected. We report results of plasma renin activity measurements and a comparison with seven renin kits, and with bioassay for plasma renin activity.

Sign in / Sign up

Export Citation Format

Share Document