Micromethods for quantitative lipid analysis of human liver needle biopsy specimens

1983 ◽  
Vol 127 (3) ◽  
pp. 373-382 ◽  
Author(s):  
Stuart R. Cairns ◽  
Timothy J. Peters
Keyword(s):  
Needle Biopsy ◽  
Human Liver ◽  
Lipid Analysis ◽  
Biopsy Specimens ◽  
Liver Needle Biopsy

QUANTITATIVE LIPID ANALYSIS OF HUMAN LIVER NEEDLE BIOPSY SPECIMENS

2009 ◽  
Vol 186 (1-6) ◽  
pp. 149-150 ◽  
Author(s):  
Antti Reunanen ◽  
Tatu A. Miettinen ◽  
Esko A. Nikkilä
Keyword(s):  
Needle Biopsy ◽  
Human Liver ◽  
Lipid Analysis ◽  
Biopsy Specimens ◽  
Liver Needle Biopsy

scholarly journals Analytical subcellular fractionation of needle-biopsy specimens from human liver

1978 ◽  
Vol 174 (2) ◽  
pp. 435-446 ◽  
Author(s):  
T J Peters ◽  
C A Seymour
Keyword(s):  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Density Gradient ◽  
Needle Biopsy ◽  
Human Liver ◽  
Resolving Power ◽  
Equilibrium Density ◽  
Marker Enzyme ◽  
Acid Hydrolases ◽  
Biopsy Specimens

1. Fragments (2-20 mg wet wt.) of closed needle-biopsy specimens from human liver were disrupted in iso-osmotic sucrose and subjected to low-speed centrifugation. The supernatant was layered on a linear sucrose-density gradient in the Beaufay small-volume automatic zonal rotor. The following organelles, with equilibrium densities (g/ml) and principal marker enzyme shown in parentheses, were resolved: plasma membrane (1.12-1.14; 5′-nucleotidase); lysosomes (1.15-1.20; N-acetyl-beta-glucosaminidase); mitochondria (1.20; malate dehydrogenase); endoplasmic reticulum (1.17-1.21; neutral alpha-glucosidase); peroxisomes (1.22-1.24; catalase). 2. The distribution of particulate alkaline phosphatase and, to a lesser degree, leucine 2-naphthylamidase followed that of 5′-nucleotidase. gamma-Glutamyltransferase was associated with membranes of significantly higher equilibrium density than was 5′-nucleotidase. 3. The distribution of 12 acid hydrolases was determined in the density-gradient fractions. beta-Glucosidase had a predominantly cytosolic localization, but the other enzymes showed a broad distribution of activity throughout the gradient. Evidence was presented for two populations of lysosomes with equilibrium densities of 1.15 and 1.20 g/ml, but containing differing amounts of each enzyme. Further evidence of lysosomal heterogeneity was demonstrated by studying the distribution of isoenzymes of hexosaminidase and of acid phosphatase. 4. The resolving power of the centrifugation procedure can be further enhanced with membrane perturbants. Digitonin (0.12 mM) selectively disrupted lysosomes, markedly increased the equilibrium density of plasma-membrane components and lowered the density of the endoplasmic reticulum, but did not affect the mitochondria or peroxisomes. Pyrophosphate (15 mM) selectively lowered the equilibrium density of the endoplasmic reticulum.

Determination of bromsulphthalein-glutathione conjugating enzyme activity in needle biopsy specimens of human liver

1973 ◽  
Vol 47 (2) ◽  
pp. 133-137 ◽  
Author(s):  
D.V. Datta ◽  
Sarban Singh ◽  
A.K.S. Samanta ◽  
S. Saha ◽  
M. Mukherjee ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Needle Biopsy ◽  
Human Liver ◽  
Biopsy Specimens ◽  
Conjugating Enzyme

Androgen receptor concentrations in needle biopsy specimens of human liver

2008 ◽  
Vol 8 (1) ◽  
pp. 28-31 ◽  
Author(s):  
P. Bannister ◽  
C. M. Meystre ◽  
M. S. Losowsky
Keyword(s):  
Androgen Receptor ◽  
Needle Biopsy ◽  
Human Liver ◽  
Biopsy Specimens

Lipid Analysis of Needle Biopsy Specimens from Skeletal Muscle of Control Subjects and Chronic Alcoholics

1982 ◽  
Vol 63 (3) ◽  
pp. 26P-26P ◽  
Author(s):  
D. Sunnasy ◽  
S.R. Cairns ◽  
F.C. Martin ◽  
G. Slavin ◽  
T.J. Peters
Keyword(s):  
Skeletal Muscle ◽  
Needle Biopsy ◽  
Lipid Analysis ◽  
Control Subjects ◽  
Biopsy Specimens
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