An immunofluorescent study of the distribution of fibronectin and laminin during limb regeneration in the adult newt

1983 ◽  
Vol 96 (2) ◽  
pp. 355-365 ◽  
Author(s):  
Adarsh K. Gulati ◽  
Andrew A. Zalewski ◽  
A.H. Reddi
Keyword(s):  
Limb Regeneration ◽  
Immunofluorescent Study ◽  
Adult Newt

The relationship between the anterior pituitary gland and the pancreas in tail regeneration of the adult newt

Development ◽  
1973 ◽  
Vol 30 (2) ◽  
pp. 415-426
Author(s):  
Swani Vethamany-Globus ◽  
Richard A. Liversage
Keyword(s):  
Pituitary Gland ◽  
Histological Examination ◽  
Anterior Pituitary ◽  
Limb Regeneration ◽  
Normal Function ◽  
Anterior Pituitary Gland ◽  
Tail Regeneration ◽  
Adult Newt ◽  
Total Inhibition ◽  
The Relationship

Histological examination of the amputated tails of 17 hypophysectomized newts revealed abnormal and extremely retarded regenerates; four of them exhibited total inhibition of regeneration. Thus, under the conditions of hypophysectomy, normal tail regeneration does not ensue in the adult Diemictylus viridescens. Also, hypophysectomy adversely affects the normal histology of both the endocrine and exocrine parts of the pancreas, as observed by the atrophy of the gland in all hypophysectomized cases. Presumably, the normal function of the gland was altered. This relationship between hypophysectomy and the atrophic pancreas suggests a possible involvement of the pancreas in tail and limb regeneration in the adult newt.

Limb regeneration in vertebrates: regulatory factors

1987 ◽  
Vol 65 (8) ◽  
pp. 726-729 ◽  
Author(s):  
Richard A. Liversage
Keyword(s):  
Immune System ◽  
Cyclic Nucleotides ◽  
Limb Regeneration ◽  
Epimorphic Regeneration ◽  
Regulatory Factors ◽  
Adult Newt ◽  
Hormonal Milieu ◽  
Newt Limb ◽  
Bioelectric Fields

Various regulatory factors are required in epimorphic regeneration of an adult newt limb. These factors (namely, amputational injury, the wound and apical epithelium, nerves (mitogenic agents), hormones (the hormonal milieu), bioelectric fields, probably the immune system, and possibly cyclic nucleotides and heretofore unknown regulators) act in concert and contribute to the developing microenvironment of the regenerate in support of normal regrowth and differentiation.

scholarly journals Reviewing the Effects of Skin Manipulations on Adult Newt Limb Regeneration: Implications for the Subcutaneous Origin of Axial Pattern Formation

Biomedicines ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1426
Author(s):  
Martin Miguel Casco-Robles ◽  
Kayo Yasuda ◽  
Kensuke Yahata ◽  
Fumiaki Maruo ◽  
Chikafumi Chiba
Keyword(s):  
Pattern Formation ◽  
Limb Regeneration ◽  
Pivotal Role ◽  
Axial Patterning ◽  
Small Areas ◽  
Larval Amphibians ◽  
Adult Newt ◽  
Newt Limb ◽  
Intact Limb

Newts are unique salamanders that can regenerate their limbs as postmetamorphic adults. In order to regenerate human limbs as newts do, it is necessary to determine whether the cells homologous to those contributing to the limb regeneration of adult newts also exist in humans. Previous skin manipulation studies in larval amphibians have suggested that stump skin plays a pivotal role in the axial patterning of regenerating limbs. However, in adult newts such studies are limited, though they are informative. Therefore, in this article we have conducted skin manipulation experiments such as rotating the skin 180° around the proximodistal axis of the limb and replacing half of the skin with that of another location on the limb or body. We found that, contrary to our expectations, adult newts robustly regenerated limbs with a normal axial pattern regardless of skin manipulation, and that the appearance of abnormalities was stochastic. Our results suggest that the tissue under the skin, rather than the skin itself, in the intact limb is of primary importance in ensuring the normal axial pattern formation in adult newt limb regeneration. We propose that the important tissues are located in small areas underlying the ventral anterior and ventral posterior skin.

Effects of radius—ulna removal on forelimb regeneration in Xenopus laevis froglets

Development ◽  
1984 ◽  
Vol 82 (1) ◽  
pp. 9-24
Author(s):  
Robert G. Korneluk ◽  
Richard A. Liversage
Keyword(s):  
Xenopus Laevis ◽  
Limb Regeneration ◽  
Cartilage Regeneration ◽  
Tissue Response ◽  
Amputation Level ◽  
Cartilage Formation ◽  
Adult Newt ◽  
Forearm Bones

Regeneration of boneless amputated forearms of adult newts was found to progress at a rate andto a degree comparable to amputated control limbs in which stump bones were not removed. In contrast, regeneration of boneless amputated Xenopus froglet forearms was significantly delayed and did not occur until two to three weeks following amputation. This is in comparisonwith the initiation of distal cartilage formation observed one week postamputation in control forelimbs of Xenopus froglets. The regeneration of cartilage in boneless forearms of adult newts wasfound to occur distal to the amputation level. In contrast, distal as well as proximal (centripetal) regeneration of cartilage was observed in the amputated boneless forearms of Xenopus. In froglets and newts, unamputated forelimbs in which forearm bones were extirpated did not initiate cartilage regeneration. Our findings support the hypothesis that forelimb regeneration in Xenopus froglets is primarily a tissue response. In comparison, limb regeneration in the adult newt is predominantly an epimorphic response.

N-Acetyl-Glucosaminidase Activity during Limb Regeneration in the Adult Newt

1981 ◽  
Vol 19 (1-3) ◽  
pp. 121-123 ◽  
Author(s):  
M. RIVERA ◽  
J.R. ORTIZ ◽  
E. ORTIZ
Keyword(s):  
Limb Regeneration ◽  
Adult Newt
Sign in / Sign up

Export Citation Format

Share Document