Electrochemical polymerization of dicarboxylic acids—III. Quantitative results in the electrolysis of adipic acid

1987 ◽  
Vol 23 (2) ◽  
pp. 133-141 ◽  
Author(s):  
A. Gozlan ◽  
A. Zilkha
Keyword(s):  
Adipic Acid ◽  
Electrochemical Polymerization ◽  
Dicarboxylic Acids ◽  
Quantitative Results

Electrochemical polymerization of dicarboxylic acids—I. Polymerization of adipic acid

1984 ◽  
Vol 20 (8) ◽  
pp. 759-763 ◽  
Author(s):  
A. Gozlan ◽  
G. Agam ◽  
S. Vardi ◽  
A. Zilkha
Keyword(s):  
Adipic Acid ◽  
Electrochemical Polymerization ◽  
Dicarboxylic Acids

scholarly journals REACTIVE EXTRACTION OF DICARBOXYLIC ACIDS I. MECHANISM, LIMITING STEPS AND KINETICS

1997 ◽  
Vol 5 (5) ◽  
pp. 97-110
Author(s):  
Dan Cascaval ◽  
Radu Tudose ◽  
Comeliu Oniscu
Keyword(s):  
Mass Transfer ◽  
Oxalic Acid ◽  
Succinic Acid ◽  
Transfer Process ◽  
Adipic Acid ◽  
Glutaric Acid ◽  
Butyl Acetate ◽  
Dicarboxylic Acids ◽  
Mass Transfer Process ◽  
Reactive Extraction

In this paper the reactive extraction of some dicarboxylic acids (oxalic acid, malonic acid, succinic acid, glutaric acid, and adipic acid) have been studied. These acids have been extracted by Amberlite LA-2 in butyl acetate using a modified extraction cell of the Lewis type. Mechanism, limiting steps, and kinetic of the mass transfer process have been settled.

scholarly journals Genetic Analysis of a Gene Cluster for Cyclohexanol Oxidation in Acinetobacter sp. Strain SE19 by In Vitro Transposition

2000 ◽  
Vol 182 (17) ◽  
pp. 4744-4751 ◽  
Author(s):  
Qiong Cheng ◽  
Stuart M. Thomas ◽  
Kristy Kostichka ◽  
James R. Valentine ◽  
Vasantha Nagarajan
Keyword(s):  
Adipic Acid ◽  
Dicarboxylic Acids ◽  
Central Carbon Metabolism ◽  
Transposon Mutagenesis ◽  
Open Reading Frames ◽  
Cosmid Library ◽  
E Coli ◽  
Transposon Mutants ◽  
In Vitro Transposon Mutagenesis

ABSTRACT Biological oxidation of cyclic alcohols normally results in formation of the corresponding dicarboxylic acids, which are further metabolized and enter the central carbon metabolism in the cell. We isolated an Acinetobacter sp. from an industrial wastewater bioreactor that utilized cyclohexanol as a sole carbon source. A cosmid library was constructed from Acinetobacter sp. strain SE19, and oxidation of cyclohexanol to adipic acid was demonstrated in recombinant Escherichia coli carrying a SE19 DNA segment. A region that was essential for cyclohexanol oxidation was localized to a 14-kb fragment on the cosmid DNA. Several putative open reading frames (ORFs) that were expected to encode enzymes catalyzing the conversion of cyclohexanol to adipic acid were identified. Whereas one ORF showed high homology to cyclohexanone monooxygenase fromAcinetobacter sp. strain NCIB 9871, most of the ORFs showed only moderate homology to proteins in GenBank. In order to assign functions of the various ORFs, in vitro transposon mutagenesis was performed using the cosmid DNA as a target. A set of transposon mutants with a single insertion in each of the ORFs was screened for cyclohexanol oxidation in E. coli. Several of the transposon mutants accumulated a variety of cyclohexanol oxidation intermediates. The in vitro transposon mutagenesis technique was shown to be a powerful tool for rapidly assigning gene functions to all ORFs in the pathway.

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