scholarly journals Evidence for the existence of a fraction of rat liver chromatin containing only a few specific nonhistone proteins and reduced amount of histone H1

FEBS Letters ◽  
1977 ◽  
Vol 81 (1) ◽  
pp. 37-38 ◽  
Author(s):  
Boika Anachkova ◽  
George Russev
1986 ◽  
Vol 41 (9-10) ◽  
pp. 910-916 ◽  
Author(s):  
Rüdiger Brust

Abstract Rat liver chromatin has been fractionated by different solubility in solvents of 155 mᴍ ionic strength in soluble S and insoluble I-chromatin. Histone H1 content is lower in S as compared to I-chromatin. The HMG1/2 nonhistone proteins are observed in S-chromatin and in the nuclear pelleted residue from the chromatin isolation procedure, but no amount can be detected in I-chromatin. Thermal denaturation profiles and CD-spectra are different for S and I-chromatin indicating distinct interactions between DNA and proteins in the chromatin molecules. Both effects, differing protein content and distinct DNA-protein interactions, can be correlated with solubility and insolubility being the result of charge-charge interactions between chromatin molecules and ionic components of the solvent.


Biochemistry ◽  
1974 ◽  
Vol 13 (25) ◽  
pp. 5128-5134 ◽  
Author(s):  
Ming Ta Chong ◽  
William T. Garrard ◽  
James Bonner

Biochemistry ◽  
1981 ◽  
Vol 20 (24) ◽  
pp. 6781-6789 ◽  
Author(s):  
Donald B. Jump ◽  
Steven Seelig ◽  
Harold L. Schwartz ◽  
Jack H. Oppenheimer

Sign in / Sign up

Export Citation Format

Share Document