Quantitative determination of in vivo endocytosis by rat liver Kupffer and endothelial cells facilitated by an improved cell isolation method (1982) FEBS Letters 141, 229-232.

To assess the contribution made by mRNA-containing particles to the heterogeneity previously observed among rat liver 40S ribonucleoprotein particles, the amount of poly(A)-containing RNA in subribosomal particles was determined. RNA was labelled with orotate in vivo for 24h and then for 50min. Poly(A)-containing RNA was trapped on filters impregnated with poly(U). Very little poly(A)-containing RNA was found in conventionally prepared ribonucleoprotein particles after fractionation in sucrose. However, after preparation of ribonucleoprotein particles by sedimentation through 1 M-sucrose in the presence of 0.15M-KCl or by precipitation with Mg2+ as described by Leitin & Lerman [(1969) Biokhimiya 34, 839-849], amounts of poly(A)-containing RNA were similar to amounts of mRNA found by other workers in total ribonucleoprotein particles. Even in such preparations, less than 5% of the total rapidly labelled RNA in native subribosomal-particle fractions was mRNA. It seems that mRNA-containing particles make up only a very small part of the population of subribosomal particles in liver.

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Quantitative determination of cyanobacteria in mixed phytoplankton assemblages by an in vivo fluorimetric method

Analytica Chimica Acta ◽

10.1016/0003-2670(94)00425-l ◽

1995 ◽

Vol 302 (1) ◽

pp. 81-87 ◽

Cited By ~ 42

Author(s):

Tsai-yun Lee ◽

Mikio Tsuzuki ◽

Toshifumi Takeuchi ◽

Kenji Yokoyama ◽

Isao Karube

Keyword(s):

Quantitative Determination ◽

Phytoplankton Assemblages ◽

Fluorimetric Method

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Quantitative determination of iodine in vivo

Acta Radiologica ◽

10.3109/00016925909171152 ◽

1959 ◽

Vol 52 (5) ◽

pp. 337-346 ◽

Cited By ~ 26

Author(s):

Paul Edholm ◽

Bertil Jacobson

Keyword(s):

Quantitative Determination

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On the existence of lipid peroxides in rat tissue

British Journal Of Nutrition ◽

10.1079/bjn19720065 ◽

1972 ◽

Vol 27 (1) ◽

pp. 19-26 ◽

Cited By ~ 12

Author(s):

J. Glavind

Keyword(s):

Adipose Tissue ◽

Methyl Linoleate ◽

Vitamin A ◽

Rat Liver ◽

Lipid Peroxides ◽

Yellow Colour ◽

Methyl Linoleate Hydroperoxide ◽

Rat Tissue

1. The colorimetric micro-adaption of the iodometric method and the colorimetric thiocyanate method for the determination of lipoperoxides were compared. Similar results were obtained when methyl linoleate hydroperoxide was tested, but when lipid from rat liver, muscle, kindney and testes was examined, substantial amounts were found by the iodometric, but almost nothing by the thiocyanate method.2. The main reason for the discrepancy between the methods seems to be that the iodometric micromethod also estimates substances other than true lipoperoxides. The presence of ubiquinone and vitamin A in the organ extracts was shown to interfere in this way in the method.3. The yellow colour which develops when retinol and its esters are tested by the iodometric micromethod is due not to liberated iodine but to conversion products of retinol.4. It is concluded that the occurrence of substantial amounts of lipoperoxides in vivo has so far been demonstarted only in the adipose tissue, and not in the parenchymatous organs of the rat.

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