On the existence of lipid peroxides in rat tissue

1. The colorimetric micro-adaption of the iodometric method and the colorimetric thiocyanate method for the determination of lipoperoxides were compared. Similar results were obtained when methyl linoleate hydroperoxide was tested, but when lipid from rat liver, muscle, kindney and testes was examined, substantial amounts were found by the iodometric, but almost nothing by the thiocyanate method.2. The main reason for the discrepancy between the methods seems to be that the iodometric micromethod also estimates substances other than true lipoperoxides. The presence of ubiquinone and vitamin A in the organ extracts was shown to interfere in this way in the method.3. The yellow colour which develops when retinol and its esters are tested by the iodometric micromethod is due not to liberated iodine but to conversion products of retinol.4. It is concluded that the occurrence of substantial amounts of lipoperoxides in vivo has so far been demonstarted only in the adipose tissue, and not in the parenchymatous organs of the rat.

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Determination of the amount of small messenger ribonucleic acid-containing particles free in liver cytoplasm

Biochemical Journal ◽

10.1042/bj1520051 ◽

1975 ◽

Vol 152 (1) ◽

pp. 51-56 ◽

Cited By ~ 2

Author(s):

B M Mullock ◽

R H Hinton

Keyword(s):

Rat Liver ◽

Ribonucleic Acid ◽

Messenger Ribonucleic Acid ◽

Ribonucleoprotein Particles

To assess the contribution made by mRNA-containing particles to the heterogeneity previously observed among rat liver 40S ribonucleoprotein particles, the amount of poly(A)-containing RNA in subribosomal particles was determined. RNA was labelled with orotate in vivo for 24h and then for 50min. Poly(A)-containing RNA was trapped on filters impregnated with poly(U). Very little poly(A)-containing RNA was found in conventionally prepared ribonucleoprotein particles after fractionation in sucrose. However, after preparation of ribonucleoprotein particles by sedimentation through 1 M-sucrose in the presence of 0.15M-KCl or by precipitation with Mg2+ as described by Leitin & Lerman [(1969) Biokhimiya 34, 839-849], amounts of poly(A)-containing RNA were similar to amounts of mRNA found by other workers in total ribonucleoprotein particles. Even in such preparations, less than 5% of the total rapidly labelled RNA in native subribosomal-particle fractions was mRNA. It seems that mRNA-containing particles make up only a very small part of the population of subribosomal particles in liver.

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Comparison of in vivo effects of insulin on SREBP-1c activation and INSIG-1/2 in rat liver and human and rat adipose tissue

Obesity ◽

10.1002/oby.20134 ◽

2013 ◽

Vol 21 (6) ◽

pp. 1208-1214 ◽

Cited By ~ 18

Author(s):

Guenther Boden ◽

Sajad Salehi ◽

Peter Cheung ◽

Carol Homko ◽

Weiwei Song ◽

...

Keyword(s):

Adipose Tissue ◽

Rat Liver ◽

Rat Adipose Tissue ◽

In Vivo Effects

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Vitamin A Supplementation During Suckling and Post-Weaning Periods Improves Systemic and Adipose Tissue Redox Status in Offspring of Rats Consuming a High-Fat Diet

Current Developments in Nutrition ◽

10.1093/cdn/nzaa041_039 ◽

2020 ◽

Vol 4 (Supplement_2) ◽

pp. 135-135

Author(s):

Yanqi Zhang ◽

Katelyn Senkus ◽

Kristi Crowe-White ◽

Libo Tan

Keyword(s):

Adipose Tissue ◽

Vitamin A ◽

High Fat Diet ◽

Lipid Peroxides ◽

Redox Status ◽

Sprague Dawley ◽

High Fat ◽

Redox Biology ◽

Metabolic Conditions ◽

Group A

Abstract Objectives Dietary fat can contribute to oxidative stress and adipose tissue (AT) accrual which exacerbates redox imbalance. Vitamin A (VA) has been shown to regulate AT development and associated metabolic conditions in adult models; however, such effects of VA in early life stages, especially on redox status, has barely been studied. The aim of this study was to assess the effects of VA supplementation during suckling and post-weaning periods on systemic and AT redox status in rats reared by mothers consuming a high-fat diet (HFD). Methods Sprague-Dawley rats arrived on their second day of gestation and were randomized to either a normal-fat diet (NFD = 25% fat) or a HFD (50% fat) both with adequate VA at 2.6 mg/kg. Upon delivery of pups, half of rat mothers from the NFD cohort or HFD cohort were switched to a NFD or a HFD both with supplemented VA at 129 mg/kg (NFD + VA and HFD + VA group), respectively. The other half of mothers remained on their diets with adequate VA (NFD and HFD group). At postnatal day 14 (P14), P25, and P35, pups (n = 4 or 3/group/time point) were euthanized. Concentrations of VA, lipid peroxides, and antioxidant capacity (AC) of serum, visceral white AT (WAT) and brown AT (BAT) were measured. Results At all three time points, the lipid peroxides concentration in WAT was significantly lower in VA-supplemented groups as compared to their respective control (P < 0.0001 for NFD + VA vs. NFD; P < 0.05 for HFD + VA vs. HFD); lipid peroxides were significantly (P < 0.01) higher in the HFD + VA group than in the NFD + VA group. A significantly (P < 0.05) positive correlation between the amount of VA and the lipophilic AC of the WAT was noted. For BAT, the NFD + VA group showed a significantly (P < 0.001) lower lipid peroxides concentration as compared to the NFD group. However, such effect was not observed in the HFD cohort. Serum lipid peroxides in the HFD + VA group were significantly (P < 0.05) lower than in the HFD group. A significant (P < 0.0001) negative correlation was found between the serum concentration of VA and lipid peroxides. Conclusions VA supplementation during suckling and post-weaning period improved both the systemic and WAT redox status of rats reared by mothers consuming a HFD, as well as the status within both WAT and BAT of pups nursed by mothers consuming a NFD. The differing results in WAT and BAT may suggest their different roles in redox biology. Funding Sources NIH

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Determination of Antioxidant Components and Activity of Tamarix ramosissima Comparative with Vaccinium myrtillus on Streptozotocin-diabetic Mice

Revista de Chimie ◽

10.37358/rc.18.7.6432 ◽

2018 ◽

Vol 69 (7) ◽

pp. 1860-1865

Author(s):

Luciana Teodora Rotaru ◽

Renata Maria Varut ◽

Mihai Banicioiu Covei ◽

Irina Iuliana Costache ◽

Marius Novac ◽

...

Keyword(s):

Lipid Peroxides ◽

Thiobarbituric Acid ◽

Vaccinium Myrtillus ◽

Tamarix Ramosissima ◽

Diabetic Mice ◽

Flavonoid Content ◽

Thiobarbituric Acid Reactive Substances ◽

Total Polyphenol

Tamarix ramosissima (Tamaricaceae) is a small tree that grows spontaneously in Europe and Asia, being considered an invasive species in geographical areas with warm climates. The chemical composition is partially elucidated, being empirically used for antiinflammatory, analgesic, antibacterial and antioxidant effect. Our study aimed to evaluate the total polyphenol and flavonoid content of vegetal extracts and to test in vivo antioxidant therapeutic effect of it, comparative with Vaccinium myrtillus, using streptozotocin-induced diabetic mice. After five weeks the animals were sacrificed and we determined erythrocyte activities of superoxide dismutase, glutathione peroxidase, glutathione reductase and level of lipid peroxides as thiobarbituric acid reactive substances. Antioxidant enzymes had highest activities in mice treated with T. ramosissima extract and the level of lipid peroxides was the lowest. The tested extract had higher content of polyphenols comparative with V. myrtillus. Our results sustain the efficiency of T. ramosissima extracts on normalizing the effects of oxidative stress in diabetes.

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