Pool size, synthesis, and turnover of sulfated and nonsulfated cholic acid and chenodeoxycholic acid in patients with cirrhosis of the liver

1. The turnover of [24−14C]cholic acid and [3H]chenodeoxycholic acid and the faecal excretion of neutral steroids were studied in six normolipaemic subjects before and during the ingestion of 1.3–2.6 mmol (0.5–1.0 g) of deoxycholic acid/day. Before the second study the subjects had been fed deoxycholic acid for 2 weeks. 2. The administration of deoxycholic acid did not appear to influence cholesterol metabolism as judged by the absence of change in the serum concentrations and the overall transformation into primary bile acids and neutral faecal steroids. 3. During the deoxycholic acid feeding period the mean total synthesis of bile acids was reduced by about 30%, corresponding to approximately 0.25 mmol (100 mg)/day. In one subject the pool size and in another the synthesis of cholic acid remained unchanged; otherwise the cholic acid pool size and its rate of formation decreased in all subjects. No consistent effects were observed with regard to the turnover of chenodeoxycholic acid. 4. Assuming that the bile acid turnover is equivalent to bile acid excretion then the total amount of cholesterol eliminated as bile acids and neutral faecal steroids averaged between 1.6 and 1.8 mmol/day before and during the administration of deoxycholic acid.

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The Formation of Deoxycholic Acid and Chenodeoxycholic Acid in Man

Clinical Science ◽

10.1042/cs0460183 ◽

1974 ◽

Vol 46 (2) ◽

pp. 183-190

Author(s):

Kurt Einarsson ◽

Kjell Hellström

Keyword(s):

Oral Administration ◽

Cholic Acid ◽

Chenodeoxycholic Acid ◽

Half Life ◽

Deoxycholic Acid ◽

Pool Size ◽

Acid Fraction ◽

Turnover Rates ◽

Mean Values ◽

The Mean

1. The turnover of deoxycholic acid and chenodeoxycholic acid was studied in six normolipaemic patients after oral administration of trace amounts of isotopically labelled compounds. 2. The mean values for half-life, pool size and turnover of deoxycholic acid were 3·0 days, 663 mg and 171 mg/day respectively. The corresponding values recorded for chenodeoxycholic acid were 2·8 days, 781 mg and 207 mg/day. 3. A comparison of the turnover rates of deoxycholic acid and cholic acid in three subjects indicated that 25–61% of the cholic acid was converted into deoxycholic acid. 4. Only trace amounts of radioactivity were recovered in the trihydroxycholanic acid fraction of duodenal bile after the administration of [14C]deoxycholic acid or [3H]chenodeoxycholic acid.

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7alpha-Dehydroxylation of cholic acid and chenodeoxycholic acid by Clostridium leptum.

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)40615-7 ◽

1979 ◽

Vol 20 (3) ◽

pp. 325-333

Author(s):

E J Stellwag ◽

P B Hylemon

Keyword(s):

Cholic Acid ◽

Chenodeoxycholic Acid

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Medical treatment of gallstones

Drug and Therapeutics Bulletin ◽

10.1136/dtb.16.18.69 ◽

1978 ◽

Vol 16 (18) ◽

pp. 69-71

Keyword(s):

Medical Treatment ◽

Bile Acids ◽

Cholic Acid ◽

Chenodeoxycholic Acid ◽

The Other ◽

Naturally Occurring ◽

Made In

Chenodeoxycholic acid (CDCA) (Chendol - Weddel) is one of two naturally occurring ‘primary’ bile acids (the other being cholic acid) made in the liver from cholesterol. CDCA is synthesised commercially from cholic acid and prescribed as gelatin-coated capsules containing 125 mg CDCA.

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Influence of Deoxycholic Acid on Biliary Lipids in Man

Clinical Science ◽

10.1042/cs0530249 ◽

1977 ◽

Vol 53 (3) ◽

pp. 249-256 ◽

Cited By ~ 5

Author(s):

J. Ahlberg ◽

B. Angelin ◽

K. Einarsson ◽

K. Hellström ◽

B. Leijd

Keyword(s):

Cholic Acid ◽

Chenodeoxycholic Acid ◽

Deoxycholic Acid ◽

Gall Bladder Disease ◽

Serum Triglyceride ◽

Significant Negative Correlation ◽

Control Subjects ◽

Cholesterol Saturation ◽

Biliary Lipid Composition ◽

Acid Administration

1. The duodenal bile acid composition was analysed in 24 control subjects and 107 patients with various types of hyperlipoproteinaemia. A highly significant negative correlation was observed between the proportions of deoxycholic acid and cholic acid as well as between deoxycholic acid and chenodeoxycholic acid. Patients with gall-bladder disease had an increased proportion of deoxycholic acid in their bile. 2. Eight control subjects were studied before and during the ingestion of 1·9 mmol (0·75 g) of deoxycholic acid daily. In these subjects a rise in the proportion of deoxycholic acid was also accompanied by a fall in the proportion of both cholic acid and chenodeoxycholic acid in duodenal bile. 3. The biliary lipid composition and cholesterol saturation was determined before and during the administration of 1·9 mmol (0·75 g) of chenodeoxycholic acid (n = 12) or deoxycholic acid (n = 8) daily for 3–4 weeks. The cholesterol saturation was decreased during the chenodeoxycholic acid ingestion whereas no change occurred in bile saturation during deoxycholic acid administration. 4. Ingestion of chenodeoxycholic acid lowered serum triglyceride and deoxycholic acid lowered the serum cholesterol.

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Radioimmunoassay of conjugated cholic acid, chenodeoxycholic acid, and deoxycholic acid from human serum, with use of 125I-labeled ligands.

Clinical Chemistry ◽

10.1093/clinchem/25.2.264 ◽

1979 ◽

Vol 25 (2) ◽

pp. 264-268 ◽

Cited By ~ 37

Author(s):

O Mäentausta ◽

O Jänne

Keyword(s):

Bile Acid ◽

Bile Acids ◽

Cholic Acid ◽

Chenodeoxycholic Acid ◽

Deoxycholic Acid ◽

Serum Samples ◽

Analytical Recovery ◽

Hepatobiliary Diseases ◽

Polyethylene Glycol Precipitation ◽

Free Serum

Abstract We describe a method for radioimmunoassay of conjugated cholic acid, chenodeoxycholic acid, and deoxycholic acid in serum. In the method, 125I-labeled bile acid conjugates are used as the tracers along with antibodies raised against individual bile acid-bovine serum albumin conjugates. Antibody-bound and free bile acids were separated by polyethylene glycol precipitation (final concentration, 125 g/L). Before radioimmunoassay, 0.1-mL serum samples were precipitated with nine volumes of ethanol, and portions from the supernate were used in the assays. The lowest measurable amounts of the bile acids, expressed as pmol/tube, were: cholic acid conjugates, 2; chenodeoxycholic acid conjugates, 0.5; and deoxycholic acid conjugates. 2. Analytical recovery of bile acids added to bile acid-free serum ranged from 85 to 110%; intra-assay and inter-assay CVs ranged from 3.2 to 5.3% and from 5.3 to 12.2%, respectively. Concentrations (mean +/- SD) of the bile acid conjugates in serum from apparently healthy women and men (in mumol/L) were: cholic acid conjugates, 0.43 +/- 0.17 (n = 126); chenodeoxycholic acid conjugates, 0.47 +/- 0.23 (n = 111); and deoxycholic acid conjugates, 0.33 +/- 0.11 (n = 96). The values for primary bile acids were greatly increased in patients with various hepatobiliary diseases.

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