Glucose, amino acids and fatty acids directly regulate ghrelin and NUCB2/nesfatin-1 in the intestine and hepatopancreas of goldfish (Carassius auratus) in vitro

2017 ◽  
Vol 206 ◽  
pp. 24-35 ◽  
Author(s):  
Juan Ignacio Bertucci ◽  
Ayelén Melisa Blanco ◽  
Luis Fabián Canosa ◽  
Suraj Unniappan
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Carassius Auratus ◽  
Goldfish Carassius Auratus

Oxoanions stimulate in vitro ovulation and signal transduction pathways in goldfish (Carassius auratus) follicles

1992 ◽  
Vol 263 (5) ◽  
pp. E943-E949 ◽  
Author(s):  
S. Y. Hsu ◽  
F. W. Goetz
Keyword(s):  
Signal Transduction ◽  
Carassius Auratus ◽  
Sodium Tungstate ◽  
Sodium Molybdate ◽  
Vanadyl Sulfate ◽  
Signal Transduction Pathways ◽  
Sodium Selenate ◽  
Sodium Chromate ◽  
Goldfish Carassius Auratus

The present study investigated the effects of a number of oxoanion compounds on in vitro ovulation of goldfish follicles and ovarian second messenger activities. Significant levels of ovulation were induced by 0.1 mM sodium chromate, 0.1 mM sodium metavanadate, 10 mM sodium molybdate, 0.1 mM sodium orthovanadate, 5 mM sodium selenate, 0.5 mM sodium tungstate, and 0.1 mM vanadyl sulfate. At levels that significantly stimulated ovulation, metavanadate, molybdate, orthovanadate, tungstate, and vanadyl sulfate also stimulated follicular phosphatidylinositol cycling and inhibited ovarian alkaline phosphatase activity. Moreover, the ovulation induced by these oxoanions was not inhibited by indomethacin (10 micrograms/ml), while ovulation induced by selenate and chromate was. In contrast, only vanadium-containing compounds significantly stimulated prostaglandin (PG) synthesis, and, in fact, selenate significantly inhibited PG production. Finally, only sodium molybdate- and vanadium-containing compounds appeared to increase follicular adenosine 3',5'-cyclic monophosphate content. While all oxoanions stimulated in vitro ovulation, they had differential effects on certain signal transduction pathways when tested at concentrations that stimulated in vitro ovulation. From the results, two basic groups could be delineated, one containing tungstate-, molybdate-, and vanadium-containing compounds and the other selenate and chromate. Thus the mechanism by which ovulation is induced by chromate and selenate may be different from that of vanadium-containing compounds, molybdate, and tungstate.(ABSTRACT TRUNCATED AT 250 WORDS)

Metabolic fate of 14C-labelled chlorinated and non-chlorinated fatty acids in goldfish (Carassius auratus)

2004 ◽  
Vol 30 (1) ◽  
pp. 13-20 ◽  
Author(s):  
H. Björn ◽  
G. Ewald ◽  
P. Sundin ◽  
C. Wesén ◽  
J. Skramstad ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Carassius Auratus ◽  
Metabolic Fate ◽  
Goldfish Carassius Auratus ◽  
Chlorinated Fatty Acids

The metabolism of acetate by rumen microorganisms

1974 ◽  
Vol 20 (2) ◽  
pp. 183-185 ◽  
Author(s):  
B. Emmanuel ◽  
L. P. Milligan ◽  
B. V. Turner
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Nucleic Acids ◽  
Glutamic Acid ◽  
Acetate Metabolism ◽  
Rumen Microorganisms ◽  
Wide Range

Rumen contents were incubated in vitro with acetate-1-14C. Significant amounts of 14C were incorporated into rumen microbial proteins, nucleic acids, and lipids. Serine, glutamic acid, methionine, and cystine were highly labeled, whereas less, or insignificant radioactivity was found in other amino acids. Acetate was incorporated into a wide range of microbial fatty acids. The quantitative significance of acetate metabolism is discussed.

scholarly journals Quantification of Phytochemicals from CommercialSpirulinaProducts and Their Antioxidant Activities

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Naif Abdullah Al-Dhabi ◽  
Mariadhas Valan Arasu
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
High Performance ◽  
Antioxidant Activities ◽  
Analytical Techniques ◽  
Essential Amino Acids

The present study aimed to profile the polyunsaturated fatty acids, sugars, free amino acids, and polyphenols in 37 varieties ofSpirulinacommonly available in the market using gas chromatography and high performance liquid chromatography. In addition, the biological potentials of theSpirulinasamples were evaluated by analysing thein vitroantioxidant activities using various analytical techniques. The analyses revealed the presence of 13 polyunsaturated fatty acids, 18 amino acids, 7 sugars, and polyphenols. The polyunsaturated fatty acids contents were varied betweenSpirulinasamples. The total polyunsaturated fatty acids amount was 4.25 mg/100 g, and the average among of sapienic acid detected was 2.25 mg/100 g, which was followed by linoleic acid (16.7%) andγ-linolenic acid (14%). Among the 7 sugars, the hexose levels were the highest (73.85%). The total amino acids contents ranged from 11.49 to 56.14 mg/100 g, and the individual essential amino acids accounted for 17% to 39.18%. The “natural” tablets exhibited the highest polyphenols levels (24 mg/g). All of theSpirulinasamples expressed dose-dependent antioxidant activities. The polyunsaturated fatty acids, sugars, free amino acids, and polyphenols contents varied widely, and the variations in these compounds between theSpirulinasamples were significant.

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