Vitellogenin gene expression in male rainbow trout (Salmo gairdneri

1988 ◽  
Vol 71 (3) ◽  
pp. 359-371 ◽  
Author(s):  
Katherine Le Guellec ◽  
Kenneth Lawless ◽  
Yves Valotaire ◽  
Michel Kress ◽  
Martin Tenniswood
Keyword(s):  
Gene Expression ◽  
Rainbow Trout ◽  
Salmo Gairdneri ◽  
Vitellogenin Gene

Estrogen-Controlled Gene Expression: Induction of Two Estrogen-Responsive Genes in the Liver of Rainbow Trout (Salmo ǵairdneri)

1989 ◽  
Vol 62 (1) ◽  
pp. 25-37 ◽  
Author(s):  
Thomas T. Chen ◽  
Duane A. Howard ◽  
Luis B. Agellon ◽  
C. M. Lin ◽  
Sherry L. Davies
Keyword(s):  
Gene Expression ◽  
Rainbow Trout ◽  
Salmo Gairdneri

Use of phenylhydrazine in the detection of responsive changes in haemoglobin isomorph abundances

1988 ◽  
Vol 66 (3) ◽  
pp. 758-762 ◽  
Author(s):  
Annette P. Byrne ◽  
A. H. Houston
Keyword(s):  
Gene Expression ◽  
Rainbow Trout ◽  
Environmental Change ◽  
Globin Gene ◽  
Salmo Gairdneri ◽  
Respiratory Response ◽  
Phenylhydrazine Hydrochloride ◽  
Total Hemoglobin ◽  
Response To Environmental Change ◽  
Globin Gene Expression

The possible masking of responsive adjustments in hemoglobin isomorph abundances was examined in rainbow trout, Salmo gairdneri. Comparison of untreated animals with specimens recovering from anemia induced by phenylhydrazine hydrochloride at 14 °C under normoxic circumstances revealed significant differences in the relative abundances of 8 of 12 isomorphs representing over half of the total hemoglobin complement. Comparisons with trout similarly treated after normoxic acclimation to 20 °C led to very similar findings. Observed differences do not appear to be attributable to direct phenylhydrazine effects on globin gene expression or to acute anemic hypoxia. It is suggested that prior induction of anemia may provide a means for improving detection of changes in hemoglobin isomorph complement during respiratory response to environmental change.

Influence of xenobiotics on rainbow trout liver estrogen receptor and vitellogenin gene expression

1995 ◽  
Vol 15 (2) ◽  
pp. 143-151 ◽  
Author(s):  
G Flouriot ◽  
F Pakdel ◽  
B Ducouret ◽  
Y Valotaire
Keyword(s):  
Gene Expression ◽  
Estrogen Receptor ◽  
Rainbow Trout ◽  
Expression Vector ◽  
Mrna Accumulation ◽  
Trout Hepatocyte ◽  
Rainbow Trout Hepatocyte ◽  
Vitellogenin Gene ◽  
Rainbow Trout Liver ◽  
Hepatocyte Primary Culture

ABSTRACT Rainbow trout hepatocyte primary culture was used to test the influence of some xenobiotics on the expression of two genes implicated in reproduction, those for the estrogen receptor (ER) and vitellogenin (Vg). We showed that chlordecone, nonylphenol, a polychlorobiphenol (PCB) mixture (Aroclor 1245) and lindane were able to induce ER and Vg mRNA accumulation. Antiestrogens, 4-hydroxytamoxifen and ICI 164,384, prevented the effects of the xenobiotics, indicating that the induction of gene expression is mediated by the ER. Among these four xenobiotics, only chlordecone and nonylphenol were able to displace the binding of [3H]estradiol to ER-enriched COS-1 extracts, and to activate an estrogen-dependent reporter gene (ERE-TK-CAT) cotransfected with an expression vector containing ER cDNA. The results suggest that chlordecone and nonylphenol are direct inducers of rainbow trout ER and Vg gene expression, whereas PCBs and lindane act through their hepatic metabolites. Moreover, pentachlorophenol acts as an antagonist of the induction by estradiol of rainbow trout ER and Vg gene expression.

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