Synaptosomes and synaptosome membrane vesicles from the brain of Mamestra configurata: Application to voltage-dependent and ATP-dependent Ca2+ ion transport studies

1987 ◽  
Vol 17 (6) ◽  
pp. 911-918 ◽  
Author(s):  
Ma Luo ◽  
Robert P. Bodnaryk
Keyword(s):  
Ion Transport ◽  
Membrane Vesicles ◽  
Mamestra Configurata ◽  
Transport Studies ◽  
Voltage Dependent ◽  
The Brain ◽  
Synaptosome Membrane

Transport Studies in Bacterial Membrane Vesicles

Science ◽  
1974 ◽  
Vol 186 (4167) ◽  
pp. 882-892 ◽  
Author(s):  
H. R. Kaback
Keyword(s):  
Membrane Vesicles ◽  
Bacterial Membrane ◽  
Transport Studies

Transport of L-proline by luminal membrane vesicles from pars recta of rabbit proximal tubule

1988 ◽  
Vol 254 (5) ◽  
pp. F628-F633
Author(s):  
H. Roigaard-Petersen ◽  
C. Jacobsen ◽  
M. I. Sheikh
Keyword(s):  
Active Site ◽  
Membrane Vesicles ◽  
Rabbit Kidney ◽  
Renal Transport ◽  
High Affinity ◽  
Luminal Membrane ◽  
Transport Studies ◽  
Straight Tubules ◽  
Pars Recta ◽  
Maximal Capacity

The mechanism of renal transport of L-proline by luminal membrane vesicles prepared from proximal straight tubules (pars recta) of rabbit kidney was investigated. The following picture emerges from transport studies: an electrogenic and Na+-requiring system confined to this region of nephron exists for transport of L-proline with a high affinity (Km = 0.16 mM) and low capacity (Vmax = 3.5 nmol.mg protein-1.15 S-1). Lowering the pH from 7.5 to 5.5 increased the affinity (Km lowered from 0.16 mM at pH 7.5 to 0.08 mM at pH 5.5) without changing the maximal capacity of this system. Modification of histidyl residues of the intact luminal membrane vesicles by diethyl-pyrocarbonate (DEP) completely abolished the transient renal accumulation of L-proline. Simultaneous presence of Na+ and L-proline (10 mM) protects against DEP inactivation of renal transport of radioactive L-proline. We propose that a histidyl residue may be at or close to the active site of L-proline transporter in vesicles from the pars recta.

Use of Isolated Membrane Vesicles in Transport Studies

Transport ◽  
1975 ◽  
pp. 117-162 ◽  
Author(s):  
Joy Hochstadt ◽  
Dennis C. Quinlan ◽  
Richard L. Rader ◽  
Chien-Chung Li ◽  
Diana Dowd
Keyword(s):  
Membrane Vesicles ◽  
Transport Studies ◽  
Isolated Membrane Vesicles
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