Black lipid membranes in aqueous media: The effect of salts on electrical properties

Abstract A pore from guinea pig heart mitochondria has been incorporated into BLM's (Black lipid membranes) in a highly oriented manner and its electrical properties studied. The pore shows multistate behaviour, the distribution of the pore between different conducting states being very sensitive to voltage. This has been proven by computation of single-pore experiments. Highest single pore conductance was 4.5 nSi in 1 ᴍ KCl, independent of voltage and with no detectable preference for cations or anions. The pore from guinea pig heart mitochondria reacts more sensitivly to voltage than pores of mitochondria from other tissues so far incorporated into BLM's.

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Black lipid membranes (BLM) in aqueous media. Photoelectric spectroscopy

The Journal of Physical Chemistry ◽

10.1021/j100714a002 ◽

1970 ◽

Vol 74 (20) ◽

pp. 3559-3568 ◽

Cited By ~ 34

Author(s):

Nguyen Thuong Van ◽

H. Ti Tien

Keyword(s):

Lipid Membranes ◽

Aqueous Media ◽

Black Lipid Membranes

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Observation of Concanavalin-A receptors on hydrated planar erythrocyte membrane film by in situ electron microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100076640 ◽

1983 ◽

Vol 41 ◽

pp. 608-609

Author(s):

Neng-Bo He ◽

S.W. Hui

Keyword(s):

Lipid Bilayers ◽

Erythrocyte Membrane ◽

Lipid Membranes ◽

Surface Film ◽

Biological Membranes ◽

Electron Microscopic Observation ◽

Electron Microscopic ◽

Black Lipid Membranes ◽

Fine Mesh ◽

Planar Films

Monolayers and planar "black" lipid membranes have been widely used as models for studying the structure and properties of biological membranes. Because of the lack of a suitable method to prepare these membranes for electron microscopic observation, their ultrastructure is so far not well understood. A method of forming molecular bilayers over the holes of fine mesh grids was developed by Hui et al. to study hydrated and unsupported lipid bilayers by electron diffraction, and to image phase separated domains by diffraction contrast. We now adapted the method of Pattus et al. of spreading biological membranes vesicles on the air-water interfaces to reconstitute biological membranes into unsupported planar films for electron microscopic study. hemoglobin-free human erythrocyte membrane stroma was prepared by hemolysis. The membranes were spreaded at 20°C on balanced salt solution in a Langmuir trough until a surface pressure of 20 dyne/cm was reached. The surface film was repeatedly washed by passing to adjacent troughs over shallow partitions (fig. 1).

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Ca2+-induced phase separation in black lipid membranes and its effect on the transport of a hydrophobic ion

Biochimica et Biophysica Acta (BBA) - Biomembranes ◽

10.1016/0005-2736(85)90237-8 ◽

1985 ◽

Vol 813 (2) ◽

pp. 221-229 ◽

Cited By ~ 10

Author(s):

Alfred Miller ◽

Günther Schmidt ◽

Hansjörg Eibl ◽

Wolfgang Knoll

Keyword(s):

Phase Separation ◽

Lipid Membranes ◽

Black Lipid Membranes

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The Use of Tethered Bilayer Lipid Membranes to Identify the Mechanisms of Antimicrobial Peptide Interactions with Lipid Bilayers

Antibiotics ◽

10.3390/antibiotics8010012 ◽

2019 ◽

Vol 8 (1) ◽

pp. 12 ◽

Cited By ~ 13

Author(s):

Amani Alghalayini ◽

Alvaro Garcia ◽

Thomas Berry ◽

Charles Cranfield

Keyword(s):

New Zealand ◽

Patch Clamp ◽

Lipid Bilayers ◽

Lipid Membranes ◽

Antimicrobial Agents ◽

Bilayer Lipid Membranes ◽

Bilayer Lipid ◽

Black Lipid Membranes ◽

Peptide Interactions ◽

New Research

This review identifies the ways in which tethered bilayer lipid membranes (tBLMs) can be used for the identification of the actions of antimicrobials against lipid bilayers. Much of the new research in this area has originated, or included researchers from, the southern hemisphere, Australia and New Zealand in particular. More and more, tBLMs are replacing liposome release assays, black lipid membranes and patch-clamp electrophysiological techniques because they use fewer reagents, are able to obtain results far more quickly and can provide a uniformity of responses with fewer artefacts. In this work, we describe how tBLM technology can and has been used to identify the actions of numerous antimicrobial agents.

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