Black lipid membranes in aqueous media: interfacial free energy measurements and effect of surfactants on film formation and stability

1967 ◽  
Vol 71 (11) ◽  
pp. 3395-3401 ◽  
Author(s):  
H. Ti Tien
Keyword(s):  
Free Energy ◽  
Lipid Membranes ◽  
Film Formation ◽  
Aqueous Media ◽  
Interfacial Free Energy ◽  
Black Lipid Membranes

scholarly journals Black Lipid Membranes at Bifaces

1968 ◽  
Vol 52 (1) ◽  
pp. 125-144 ◽  
Author(s):  
H. Ti Tien
Keyword(s):  
Free Energy ◽  
Optical Properties ◽  
Lipid Membranes ◽  
Biological Membrane ◽  
Spontaneous Reduction ◽  
Black Lipid Membranes ◽  
Realistic Approach ◽  
Oil Water ◽  
Membrane Models

Black lipid membranes (BLM) less than 90 A thick have been shown to be the most realistic approach to biological membrane models. This paper describes the formation characteristics, optical properties, and thermodynamics of BLM at water/oil/water bifaces. In particular, the nature of the Plateau-Gibbs border which supports the black membrane is analyzed in some detail. The formation of BLM at the biface involves a spontaneous reduction of the free energy of the system. As long as the integrity of the membrane is maintained, the limiting structure of the BLM represents the lowest free energy configuration.

Observation of Concanavalin-A receptors on hydrated planar erythrocyte membrane film by in situ electron microscopy

1983 ◽  
Vol 41 ◽  
pp. 608-609
Author(s):  
Neng-Bo He ◽  
S.W. Hui
Keyword(s):  
Lipid Bilayers ◽  
Erythrocyte Membrane ◽  
Lipid Membranes ◽  
Surface Film ◽  
Biological Membranes ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Black Lipid Membranes ◽  
Fine Mesh ◽  
Planar Films

Monolayers and planar "black" lipid membranes have been widely used as models for studying the structure and properties of biological membranes. Because of the lack of a suitable method to prepare these membranes for electron microscopic observation, their ultrastructure is so far not well understood. A method of forming molecular bilayers over the holes of fine mesh grids was developed by Hui et al. to study hydrated and unsupported lipid bilayers by electron diffraction, and to image phase separated domains by diffraction contrast. We now adapted the method of Pattus et al. of spreading biological membranes vesicles on the air-water interfaces to reconstitute biological membranes into unsupported planar films for electron microscopic study. hemoglobin-free human erythrocyte membrane stroma was prepared by hemolysis. The membranes were spreaded at 20°C on balanced salt solution in a Langmuir trough until a surface pressure of 20 dyne/cm was reached. The surface film was repeatedly washed by passing to adjacent troughs over shallow partitions (fig. 1).

scholarly journals Curcuminoid-Tailored Interfacial Free Energy of Hydrophobic Fibers for Enhanced Biological Properties

2021 ◽  
Author(s):  
Wevernilson F. de Deus ◽  
Bruna M. de França ◽  
Josué Sebastian B. Forero ◽  
Alessandro E. C. Granato ◽  
Henning Ulrich ◽  
...  
Keyword(s):  
Free Energy ◽  
Interfacial Free Energy ◽  
Biological Properties

scholarly journals The Use of Tethered Bilayer Lipid Membranes to Identify the Mechanisms of Antimicrobial Peptide Interactions with Lipid Bilayers

Antibiotics ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 12 ◽  
Author(s):  
Amani Alghalayini ◽  
Alvaro Garcia ◽  
Thomas Berry ◽  
Charles Cranfield
Keyword(s):  
New Zealand ◽  
Patch Clamp ◽  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Antimicrobial Agents ◽  
Bilayer Lipid Membranes ◽  
Bilayer Lipid ◽  
Black Lipid Membranes ◽  
Peptide Interactions ◽  
New Research

This review identifies the ways in which tethered bilayer lipid membranes (tBLMs) can be used for the identification of the actions of antimicrobials against lipid bilayers. Much of the new research in this area has originated, or included researchers from, the southern hemisphere, Australia and New Zealand in particular. More and more, tBLMs are replacing liposome release assays, black lipid membranes and patch-clamp electrophysiological techniques because they use fewer reagents, are able to obtain results far more quickly and can provide a uniformity of responses with fewer artefacts. In this work, we describe how tBLM technology can and has been used to identify the actions of numerous antimicrobial agents.

Pump and displacement currents of reconstituted ATP synthase on black lipid membranes

1988 ◽  
Vol 104 (2) ◽  
pp. 179-191 ◽  
Author(s):  
B. Christensen ◽  
M. Gutweiler ◽  
E. Grell ◽  
N. Wagner ◽  
R. Pabst ◽  
...  
Keyword(s):  
Atp Synthase ◽  
Lipid Membranes ◽  
Black Lipid Membranes ◽  
Displacement Currents

Black Lipid Membranes from Polymerizable Lipids

1982 ◽  
Vol 21 (S5) ◽  
pp. 869-880 ◽  
Author(s):  
Roland Benz ◽  
Werner Pra� ◽  
Helmut Ringsdorf
Keyword(s):  
Lipid Membranes ◽  
Black Lipid Membranes ◽  
Polymerizable Lipids
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