Primary immune response of guinea pig spleen cells in vitro

1978 ◽  
Vol 20 ◽  
pp. 131-141 ◽  
Author(s):  
J. Kettman ◽  
S. Ben-Sasson ◽  
J.U. Rudin
Keyword(s):  
Immune Response ◽  
Guinea Pig ◽  
Primary Immune Response ◽  
Spleen Cells

scholarly journals CELL INTERACTIONS IN THE PRIMARY IMMUNE RESPONSE IN VITRO: A REQUIREMENT FOR SPECIFIC CELL CLUSTERS

1969 ◽  
Vol 129 (2) ◽  
pp. 351-362 ◽  
Author(s):  
Donald E. Mosier
Keyword(s):  
Immune Response ◽  
Cell Cultures ◽  
Primary Immune Response ◽  
Antigenic Determinants ◽  
Specific Cell ◽  
Spleen Cells ◽  
Sheep Erythrocytes ◽  
Cell Clusters ◽  
Immune Response In Vitro

Mouse spleen cells were found to associate in cell clusters during the primary immune response to sheep erythrocytes in vitro. About 10% of the cell clusters had the following unique properties; (a) they contained most, if not all, antibody-forming cells, (b) they contained only cells forming antibody to one antigen when cell cultures were immunized with two antigens, (c) the cells in clusters reaggregated specifically after dispersion, and (d) the specific reaggregation of clusters appeared to be blocked by antibody to the antigen. The integrity of cell clusters was required for the proliferation of antibody-forming cells, and prevention of clustering by mechanical means or by excess antibody blocked the immune response. Antibody and antigenic determinants on the surfaces of cells probably provide the basis for interaction. The unique microenvironment of cell clusters was essential for the primary immune response in vitro.

scholarly journals EFFECTS OF HYDROCORTISONE ON THE IN VITRO PRIMARY IMMUNE RESPONSE OF MOUSE SPLEEN CELLS TO SHEEP ERYTHROCYTES

1972 ◽  
Vol 25 (5) ◽  
pp. 345-353 ◽  
Author(s):  
MASANOBU SUGIMOTO ◽  
SHIN-ICHI TAMURA ◽  
TAKESHI KURATA ◽  
YASUYUKI EGASHIRA
Keyword(s):  
Immune Response ◽  
Primary Immune Response ◽  
Mouse Spleen ◽  
Spleen Cells ◽  
Sheep Erythrocytes

scholarly journals The role of fetal calf serum in the primary immune response in vitro.

1977 ◽  
Vol 145 (4) ◽  
pp. 1029-1038 ◽  
Author(s):  
H G Opitz ◽  
U Opitz ◽  
H Lemke ◽  
G Hewlett ◽  
W Schreml ◽  
...  
Keyword(s):  
Immune Response ◽  
Culture Medium ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Primary Immune Response ◽  
Spleen Cells ◽  
Humoral Immune ◽  
Serum Component ◽  
Immune Response In Vitro

The mode of action of 2-mercaptoethanol (2-ME) on the primary immune response in vitro was investigated. Fetal calf serum (FCS) was preincubated with 2-ME and lyophilized to remove free 2-ME. This 2-ME-treated FCS was able to substitute the function of adherent cells in the primary immune response against sheep red blood cells (SRBC) in vitro; Fractionation of 2-tme-treated FCS on a Sephadex G-100 column showed that 2-ME acted on a high molecular serum component which after activation, could substitute for macrophages. In order to obtain a humoral immune response against SRBC in vitro, spleen cells require selected FCS. These "good" sera could be distinguished from "deficient" sera by their higher content of this 2-ME-activated factor. The height of the in vitro immune response to SRBC was dependent on the amount of activated factor added to the culture medium. FCS normally required in the culture medium could be completely replaced by the factor-containing fraction without deleterious effect on the culture medium. The factor should be added to the spleen cells during the first 24 h of culture and remain there for 72 h in order to obtain an optimal immune response. The factor could be partially absorbed by spleen cells but not by SRBC. The relationship between macrophage, 2-ME, and FCS in eliciting an in vitro primary immune response is discussed.

scholarly journals GENETIC CONTROL OF IMMUNE RESPONSES IN VITRO

1974 ◽  
Vol 140 (3) ◽  
pp. 648-659 ◽  
Author(s):  
Judith A. Kapp ◽  
Carl W. Pierce ◽  
Stuart Schlossman ◽  
Baruj Benacerraf
Keyword(s):  
Immune Response ◽  
T Cells ◽  
Immune Responses ◽  
Cell Function ◽  
Suppressor Cells ◽  
Specific Response ◽  
Spleen Cells ◽  
X Irradiation

In recent studies we have found that GAT not only fails to elicit a GAT-specific response in nonresponder mice but also specifically decreases the ability of nonresponder mice to develop a GAT-specific PFC response to a subsequent challenge with GAT bound to the immunogenic carrier, MBSA. Studies presented in this paper demonstrate that B cells from nonresponder, DBA/1 mice rendered unresponsive by GAT in vivo can respond in vitro to GAT-MBSA if exogenous, carrier-primed T cells are added to the cultures. The unresponsiveness was shown to be the result of impaired carrier-specific helper T-cell function in the spleen cells of GAT-primed mice. Spleen cells from GAT-primed mice specifically suppressed the GAT-specific PFC response of spleen cells from normal DBA/1 mice incubated with GAT-MBSA. This suppression was prevented by pretreatment of GAT-primed spleen cells with anti-θ serum plus C or X irradiation. Identification of the suppressor cells as T cells was confirmed by the demonstration that suppressor cells were confined to the fraction of the column-purified lymphocytes which contained θ-positive cells and a few non-Ig-bearing cells. The significance of these data to our understanding of Ir-gene regulation of the immune response is discussed.

scholarly journals Immunologic stimulation of early murine hematopoiesis and its abrogation by cyclosporin A

Blood ◽  
1982 ◽  
Vol 59 (4) ◽  
pp. 851-856 ◽  
Author(s):  
SA Burstein ◽  
SK Erb ◽  
JW Adamson ◽  
LA Harker
Keyword(s):  
Immune Response ◽  
Cyclosporin A ◽  
Progenitor Cells ◽  
Pokeweed Mitogen ◽  
Normal Rabbit Serum ◽  
Lymphocyte Function ◽  
Spleen Cells ◽  
Stimulation Of

Abstract Mice injected chronically with antiplatelet serum develop an increase in the number of megakaryocytic progenitor cells compared to animals given normal rabbit serum. To examine the specificity of this response, progenitor cells giving rise to megakaryocyte, granulocyte-macrophage, erythroid, and mixed-cell colonies were assayed after injection of various heterosera or saline. All four colony types increased in the serum-treated groups. Since the in vitro proliferation of hematopoietic progenitor cells is promoted by supernatants of mitogen-stimulated spleen cells, we hypothesized that the immune response following antiserum administration resulted in the in vivo activation of T lymphocytes which produced or led to the production of colony stimulating activities. To test this hypothesis, cyclosporin A, a preferential inhibitor of T lymphocyte function, was given to mice concurrently with antiserum and also added to spleen cell cultures in the presence of pokeweed mitogen. Cyclosporin A abrogated the antiserum- related increases in progenitor cell numbers in vivo and the production of colony stimulating activity in vitro. The results suggest that the immune response related to antiserum administration results in the in vivo production of hematopoietic colony stimulating activities that may be identical to those produced in vitro by mitogen-stimulation of spleen cells.

Sign in / Sign up

Export Citation Format

Share Document